Study of imunnomodulatory and antimicrobial anti-Candida actions of Lactobacillus casei

• Grant number: 11/01122-7
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2011 - June 30, 2013
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Antonio Olavo Cardoso Jorge
• Grantee: Antonio Olavo Cardoso Jorge
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
• Associated researchers: Adeline Lacerda Jorjão ; Juliana Campos Junqueira ; Luciane Dias de Oliveira ; Mariella Vieira Pereira Leão
• Associated scholarship(s): 11/14648-7 - STUDY OF IMUNNOMODULATORY AND ANTIMICROBIAL ANTI-Candida ACTIONS OF Lactobacillus casei, BP.TT

Abstract

Probiotics are dietary supplements constituted of live microorganisms or microbial cells components that are benefic to the health of the host. Since probiotics consumption seems to assist in eliminating of yeasts of Candida genus and in recovering of immune balance, this work aims to study, in vivo and in vitro, the immunomodulatory and antimicrobial anti-Candida actions of Lactobacillus casei, considered a probiotic species. For this, suspensions of Candida will be inoculated in the oral cavity of experimentally immunosuppressed mice for induction of candidosis. The animals shall be divided into four groups: the) with induction of candidosis without consuming probiotic, b) with induction of candidosis, with consumption of probiotic since 14 days before inoculation of Candida, c) with induction of candidosis with probiotic consumption during the inoculation of Candida, d) without inducing candidosis with probiotic consumption throughout the period of experimentation. Development and recovery of disease shall be histologically investigated through collecting and counting of yeasts in the oral mucosa. The presence of cells TCD4 +, TCD8 + and NKT will be investigated by Immunohistochemistry. In addition, serum samples will be collected from animals to analyze of nitric oxide, using Griess reagent, and INF-gamma, TNF-±, ² IL-1, IL-4, IL-6, IL-10, IL-12 and total immunoglobulins, by ELISA technique. Antimicrobial action anti-Candida of L. casei also will be investigated in vitro, using: agar diffusion, planctonic growth inhibition, inhibition of adhesion on epithelial cells and inhibition of the formation of biofilm. The ability of L. casei to induce the release of TNF-±, ² IL-1, IL-4, IL-6, IL-10, IL-12 and nitric oxide by macrophages (RAW 264.7) will be also investigated. (AU)