Investigation of the role of oxidative stress ND the CNBP protein in treacle-deficient mesenchymal stem-cells and in zebrafish models

• Grant number: 10/52446-4
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2011
• End date: July 31, 2013
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Agreement: CONICET
• Principal Investigator: Maria Rita dos Santos e Passos Bueno
• Grantee: Maria Rita dos Santos e Passos Bueno
• Principal researcher abroad: Nora Beatriz Calcaterra
• Institution abroad: Universidad Nacional de Rosario (UNR), Argentina
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 98/14254-2 - The Human Genome Research Center, AP.CEPID

Abstract

This Project aims to initiate a collaborative work between Dr. Passos-Bueno's and Dr. Calcaterra's laboratories, which both are interested in understand the molecular basis of developmental pathways and disorders. In this study, the groups will investigate the role of oxidative stress (OS) and the protein CNBP in zebrafish models and in mesenchymal stem cells obtained from patients bearing a congenital craniofacial malformation disorder (Treacher Collins syndrome). In Sao Paulo, we will follow our own established protocols for obtaining surgery tissue and maintaining primary mesenchymal cell cultures, as well as to establish induced pluripotent stem cell lines for functional studies. Dr Calcaterras' students will study the mechanisms of CNPB protein in the human cells obtained and cultivated by our group. Dr. Passos-Bueno's students will validate the in vitro findings with human stem cells in appropriate zebrafish models, a methodology that is already established in the Argentinean group. (AU)