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Development and use of SSR and DArT genetic markers to study genetic diversity in macadamia (Macadamia integrifolia)

Grant number: 11/06141-0
Support type:Regular Research Grants
Duration: July 01, 2011 - June 30, 2013
Field of knowledge:Biological Sciences - Genetics
Principal Investigator:Antonio Augusto Franco Garcia
Grantee:Antonio Augusto Franco Garcia
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Assoc. researchers:Anete Pereira de Souza ; Graciela da Rocha Sobierajski


The macadamia (Macadamia integrifolia) is a nut crop tree widespread across eastern Australia.Although, it was introduced in Brazil in 1931, just in the past 20 years its cultivated area hasexpanded more signicantly. However, 80% of the orchards consists of exotic varieties, and just 17local cultivars are available to farmers. Therefore, the lack of local varieties makes indispensable the development of new varieties adapted to local conditions. The macadamia tree is a perennial species that takes a long time to reproduce. Thus, traditional breeding programms takes a long time to deliver new varieties. Molecular techniques could provide genetic information useful for breeding programms, such as, the kinship (relatedness) between ndividuals, detection of quantitative trait loci (QTL) and marker assisted selection. The objective of this study is to develop both microsatellites(SSR) and diversity array technology (DArT) primers that will be used to gain insigths intothe genetic architecture of macadamia that ultimately will be applied to our breeding programm. (AU)