Advanced search
Start date
Betweenand

Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis

Abstract

While it has been clearly demonstrated the role of functional genomics on tumorigenesis, the biological behavior of some oncogenes, tumoral invasiveness, DNA repair kinetics and content along time course oral cancer progression are not fully established. Thus far, the goal of this project will be to investigate the role of some oncogenes, cellular proliferation control, DNA repair kinetics and content during experimental rat oral carcinogenesis. In this regard, a total of 30 male Wistar rats will be exposed to carcinogen 4-nitroquinoline 1-oxide by drinking water at 50 ppm dose and then, they will be sacrificed at four, 12 and 20 weeks post-treatment. A total of 30 rats will be used as negative control. Herein, it will be evaluated mutations in H-ras and K-ras oncogenes, expression of p21RAS, ki-67 e alpha-SMA, through immunohistochemistry and global genome content and DNA repair system performance by single cell gel (comet) assay during rat tongue carcinogenesis. (AU)

Scientific publications (30)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
KAHAN, V.; RIBEIRO, D. A.; EGYDIO, F.; BARROS, L. A.; TOMIMORI, J.; TUFIK, S.; ANDERSEN, M. L. Is Lack of Sleep Capable of Inducing DNA Damage in Aged Skin?. SKIN PHARMACOLOGY AND PHYSIOLOGY, v. 27, n. 3, p. 127-131, 2014. Web of Science Citations: 4.
TENORIO, NEULI M.; RIBEIRO, DANIEL A.; ALVARENGA, TATHIANA A.; FRACALOSSI, ANA CAROLINA C.; CARLIN, VIVIANE; HIROTSU, CAMILA; TUFIK, SERGIO; ANDERSEN, MONICA L. The influence of sleep deprivation and obesity on DNA damage in female Zucker rats. Clinics, v. 68, n. 3, p. 385-389, 2013. Web of Science Citations: 7.
JULIANA SOARES ROTER MARINS; LUCIANA MOURA SASSONE; SANDRA RIVERA FIDEL; DANIEL ARAKI RIBEIRO. In vitro genotoxicity and cytotoxicity in murine fibroblasts exposed to EDTA, NaOCl, MTAD and citric acid. Brazilian Dental Journal, v. 23, n. 5, p. -, Out. 2012.
MINICUCCI, E. M.; RIBEIRO, D. A.; DA SILVA, G. N.; PARDINI, M. I. M. C.; MONTOVANI, J. C.; SALVADORI, D. M. F. The role of the TP53 gene during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide. EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY, v. 63, n. 5, p. 483-489, JUL 2011. Web of Science Citations: 11.
NAGAOKA, MARCIA R.; LE SUEUR-MALUF, LUCIANA; AGUIAR, JR., ODAIR; CASTRO, GLAUCIA M.; SPADARI-BRATFISCH, REGINA C.; RIBEIRO, DANIEL A. Differential response related to genotoxicity in multiple organs of cirrhotic rats. HEPATOLOGY INTERNATIONAL, v. 5, n. 2, p. 740-746, JUN 2011. Web of Science Citations: 5.
RIBEIRO, DANIEL A.; SANNOMIYA, EDUARDO K.; POZZI, RENAN; MIRANDA, SANDRA R.; ANGELIERI, FERNANDA. Cellular death but not genetic damage in oral mucosa cells after exposure to digital lateral radiography. CLINICAL ORAL INVESTIGATIONS, v. 15, n. 3, p. 357-360, JUN 2011. Web of Science Citations: 9.
AGUIAR, JR., ODAIR; BOIAGO GOLLUECKE, ANDREA PITTELLI; DE MORAES, BARBARA BUENO; PASQUINI, GABRIELA; CATHARINO, RODRIGO RAMOS; RICCIO, MARIA FRANCESCA; MIKI IHARA, SILVIA SAIULI; RIBEIRO, DANIEL ARAKI. Grape juice concentrate prevents oxidative DNA damage in peripheral blood cells of rats subjected to a high-cholesterol diet. BRITISH JOURNAL OF NUTRITION, v. 105, n. 5, p. 694-702, MAR 2011. Web of Science Citations: 22.
HIROTSU, CAMILA; TUFIK, SERGIO; RIBEIRO, DANIEL A.; ALVARENGA, TATHIANA A.; ANDERSEN, MONICA L. Genomic damage in the progression of chronic kidney disease in rats. BRAIN BEHAVIOR AND IMMUNITY, v. 25, n. 3, p. 416-422, MAR 2011. Web of Science Citations: 10.
ANGELIERI, FERNANDA; GONCALVES MOLEIRINHO, TATIANA DE CASSIA; CARLIN, VIVIANE; FUJIYAMA OSHIMA, CELINA TIZUKO; RIBEIRO, DANIEL ARAKI. Biomonitoring of oral epithelial cells in smokers and non-smokers submitted to panoramic X-ray: comparison between buccal mucosa and lateral border of the tongue. CLINICAL ORAL INVESTIGATIONS, v. 14, n. 6, p. 669-674, DEC 2010. Web of Science Citations: 21.
MARTINS, RENATO A.; GOMES, GUILHERME A. S.; AGUIAR, JR., ODAIR; MEDALHA, CARLA C.; RIBEIRO, DANIEL A. Chromosome damage and cytotoxicity in oral mucosa cells after 2 months of exposure to anabolic steroids (decadurabolin and winstrol) in weight lifting. Steroids, v. 75, n. 12, p. 952-955, DEC 2010. Web of Science Citations: 7.
POZZI, RENAN; ROSA, JOSE C.; EGUCHI, RICARDO; OLLER DO NASCIMENTO, CLAUDIA M.; OYAMA, LILA M.; AGUIAR, JR., ODAIR; CHAVES, MARCELO D.; RIBEIRO, DANIEL A. Genetic damage in multiple organs of acutely exercised rats. Cell Biochemistry and Function, v. 28, n. 8, p. 632-636, DEC 2010. Web of Science Citations: 10.
ANGELIERI, F.; CARLIN, V.; SAEZ, D. M.; POZZI, R.; RIBEIRO, D. A. Mutagenicity and cytotoxicity assessment in patients undergoing orthodontic radiographs. Dentomaxillofacial Radiology, v. 39, n. 7, p. 437-440, OCT 2010. Web of Science Citations: 17.
ZEFERINO, E. G.; BUENO, C. E. S.; OYAMA, L. M.; RIBEIRO, D. A. Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide. International Endodontic Journal, v. 43, n. 10, p. 843-848, OCT 2010. Web of Science Citations: 19.
MATSUMOTO, MARIZA A.; CASTANHO, JULIANE; KAWAKAMI, ROBERTO Y.; RIBEIRO, DANIEL A. Cytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis. GERODONTOLOGY, v. 27, n. 3, p. 183-188, SEP 2010. Web of Science Citations: 2.
CARLIN, V.; ARTIOLI, A. J.; MATSUMOTO, M. A.; FILHO, H. N.; BORGO, E.; OSHIMA, C. T. F.; RIBEIRO, D. A. Biomonitoring of DNA damage and cytotoxicity in individuals exposed to cone beam computed tomography. Dentomaxillofacial Radiology, v. 39, n. 5, p. 295-299, JUL 2010. Web of Science Citations: 15.
KAHAN, VANESSA; RIBEIRO, DANIEL ARAKI; ANDERSEN, MONICA LEVY; ALVARENGA, TATHIANA APARECIDA; TUFIK, SERGIO. Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains. BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY, v. 107, n. 1, p. 598-602, JUL 2010. Web of Science Citations: 10.
NISHI, ERIKA EMY; CAMPOS, RUY RIBEIRO; BERGAMASCHI, CASSIA TOLEDO; DE ALMEIDA, VITOR ROSSI; RIBEIRO, DANIEL ARAKI. Vitamin C prevents DNA damage induced by renovascular hypertension in multiple organs of Wistar rats. HUMAN & EXPERIMENTAL TOXICOLOGY, v. 29, n. 7, p. 593-599, JUL 2010. Web of Science Citations: 17.
RIBEIRO, DANIEL A.; NASCIMENTO, FABIO D.; FRACALOSSI, ANA CAROLINA C.; GOMES, THIAGO S.; OSHIMA, CELINA T. F.; FRANCO, MARCELLO F. Expression Profiling of Cell Cycle Regulatory Proteins in Oropharyngeal Carcinomas Using Tissue Microarrays. IN VIVO, v. 24, n. 3, p. 315-320, MAY-JUN 2010. Web of Science Citations: 4.
ANDERSEN, MONICA L.; RIBEIRO, DANIEL A.; ALVARENGA, TATHIANA A.; SILVA, ANDRESSA; ARAUJO, PAULA; ZAGER, ADRIANO; TENORIO, NEULI M.; TUFIK, SERGIO. Are endogenous sex hormones related to DNA damage in paradoxically sleep-deprived female rats?. Hormones and Behavior, v. 57, n. 2, p. 216-221, FEB 2010. Web of Science Citations: 9.
CUZZUOL FRACALOSSI, ANA CAROLINA; MIRANDA, SANDRA REGINA; FUJIYAMA OSHIMA, CELINA TIJUKO; FRANCO, MARCELLO; RIBEIRO, DANIEL ARAKI. The role of matrix metalloproteinases 2 and 9 during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide. Journal of Molecular Histology, v. 41, n. 1, p. 19-25, FEB 2010. Web of Science Citations: 17.
CUZZUOL FRACALOSSI, ANA CAROLINA; SILVA, MARCELO DE SOUZA; FUJIYAMA OSHIMA, CELINA TIJUKO; RIBEIRO, DANIEL ARAKI. Wnt/beta-catenin signalling pathway following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide. Experimental and Molecular Pathology, v. 88, n. 1, p. 176-183, FEB 2010. Web of Science Citations: 16.
ALVARENGA, TATHIANA A.; ANDERSEN, MONICA L.; RIBEIRO, DANIEL A.; ARAUJO, PAULA; HIROTSU, CAMILA; COSTA, JOSE L.; BATTISTI, MURILO C.; TUFIK, SERGIO. Single exposure to cocaine or ecstasy induces DNA damage in brain and other organs of mice. ADDICTION BIOLOGY, v. 15, n. 1, p. 96-99, JAN 2010. Web of Science Citations: 29.
RIBEIRO, DANIEL ARAKI; CUZZUOL FRACALOSSI, ANA CAROLINA; UATARI, SILVIA AKEMI; FUJIYAMA OSHIMA, CELINA TIZUKO; FAVERO SALVADORI, DAISY MARIA. Imbalance of Tumor Suppression Genes Expression Following Rat Tongue Carcinogenesis Induced by 4-Nitroquinoline 1-Oxide. IN VIVO, v. 23, n. 6, p. 937-942, NOV-DEC 2009. Web of Science Citations: 4.
MARTINS, RENATO A.; DA SILVA GOMES, GUILHERME A.; AGUIAR, JR., ODAIR; RIBEIRO, DANIEL A. Biomonitoring of oral epithelial cells in petrol station attendants: Comparison between buccal mucosa and lateral border of the tongue. Environment International, v. 35, n. 7, p. 1062-1065, OCT 2009. Web of Science Citations: 22.
ANDERSEN, M. L.; RIBEIRO, D. A.; BERGAMASCHI, C. T.; ALVARENGA, T. A.; SILVA, A.; ZAGER, A.; CAMPOS, R. R.; TUFIK, S. Distinct effects of acute and chronic sleep loss on DNA damage in rats. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY, v. 33, n. 3, p. 562-567, APR 30 2009. Web of Science Citations: 36.
RIBEIRO, D. A.; CAMPOS, R. R.; BERGAMASCHI, C. T. Chronic renal failure induces genetic instability in multiple organs of Wistar rats. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, v. 39, n. 4, p. 289-295, APR 2009. Web of Science Citations: 10.
RIBEIRO, DANIEL A.; BUTTROS, JULIANA B.; OSHIMA, CELINA T. F.; BERGAMASCHI, CASSIA T.; CAMPOS, RUY R. Ascorbic acid prevents acute myocardial infarction induced by isoproterenol in rats: role of inducible nitric oxide synthase production. Journal of Molecular Histology, v. 40, n. 2, p. 99-105, APR 2009. Web of Science Citations: 29.
MINICUCCI, ELIANA MARIA; DA SILVA, GLENDA NICIOLI; RIBEIRO, DANIEL ARAKI; FAVERO SALVADORI, DAISY MARIA. No mutations found in exon 2 of gene p16CDKN2A during rat tongue carcinogenesis induced by 4-nitroquinoline-1-oxide. Journal of Molecular Histology, v. 40, n. 1, p. 71-76, FEB 2009. Web of Science Citations: 6.
RIBEIRO, D. A.; CARLIN, V.; FRACALOSSI, A. C. C.; OYAMA, L. M. Radiopacifiers do not induce genetic damage in murine fibroblasts: an in vitro study. International Endodontic Journal, v. 42, n. 11, p. 987-991, 2009. Web of Science Citations: 10.
RIBEIRO, DANIEL A.; GRILLI, DANIELA G.; SALVADORI, DAISY M. F. Genomic instability in blood cells is able to predict the oral cancer risk: an experimental study in rats. Journal of Molecular Histology, v. 39, n. 5, p. 481-486, OCT 2008. Web of Science Citations: 28.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.