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New challenge for the diagnosis of diarrheagenic Escherichia coli: recombinant antibody obtention against different virulence factors

Grant number: 11/12928-2
Support type:Regular Research Grants
Duration: November 01, 2011 - October 31, 2013
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Roxane Maria Fontes Piazza
Grantee:Roxane Maria Fontes Piazza
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Assoc. researchers:Denise Silvina Piccini Quintas Horton ; Patricia Antonia Estima Abreu de Aniz ; Waldir Pereira Elias Junior

Abstract

Acute diarrheal infections are still identified as a significant cause of morbidity and mortality. Annually is estimated approximately 2 to 2.5 million deaths associated with diarrhea in children under five years. Among the pathogens, diarrheagenic Escherichia coli account for 30 to 40% of the acute diarrhea episodes in developing countries. In this context, the diagnosis is an important tool to minimize and control occurrences. However, while V. cholerae, Shigella spp, and rotavirus can be detected by standard tests, the detection of E. coli is more difficult, and the fact that, mistakenly, this pathogen is not usually regarded as the leading cause of diarrhea in children. Among the diarrheagenic E. coli, the categories of epidemiological relevance and importance are: enterotoxigenic E. coli (ETEC) that has as its main virulence factors the production of heat-stable toxins (ST) and heat-labile (LT), enteropathogenic E. coli (EPEC) has intimin and Shiga toxin producing E. coli (STEC) produces the potent Shiga cytotoxins (Stx1 and / or stx2). Antibodies are important tools in the detection of various pathogens, the recombinant antibodies enter as a new option for rapid detection of pathogens, thus improving their immunodiagnostic through its virulence factors. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LUZ, DANIELA; AMARAL, MARIA MARTA; SACERDOTI, FLAVIA; BERNAL, ALAN MAURO; QUINTILIO, WAGNER; MORO, ANA MARIA; PALERMO, MARINA SANDRA; IBARRA, CRISTINA; FONTES PIAZZA, ROXANE MARIA. Human Recombinant Fab Fragment Neutralizes Shiga Toxin Type 2 Cytotoxic Effects in vitro and in vivo. TOXINS, v. 10, n. 12 DEC 2018. Web of Science Citations: 1.
LUZ, DANIELA; SHIGA, EMERSON A.; CHEN, GANG; QUINTILIO, WAGNER; ANDRADE, FERNANDA B.; MARANHAO, ANDREA Q.; CAETANO, BRUNA A.; MITSUNARI, THAIS; SILVA, MIRIAM A.; ROCHA, LETICIA B.; MORO, ANA M.; SIDHU, SACHDEV S.; PIAZZA, ROXANE M. F. Structural Changes in Stx1 Engineering Monoclonal Antibody Improves Its Functionality as Diagnostic Tool for a Rapid Latex Agglutination Test. ANTIBODIES, v. 7, n. 1 MAR 2018. Web of Science Citations: 0.
YAMAMOTO, BRUNO B.; LUZ, DANIELA; ABREU, PATRICIA A. E.; GOTTI, TATIANA B.; VASCONCELLOS, SILVIO A.; PIAZZA, ROXANE M. P.; HORTON, DENISE S. P. Q. Antibodies to Shiga toxins in Brazilian cattle. Toxicon, v. 133, p. 58-62, JUL 2017. Web of Science Citations: 0.
CAETANO, BRUNA ALVES; ROCHA, LETICIA BARBOZA; CARVALHO, ENEAS; FONTES PIAZZA, ROXANE MARIA; LUZ, DANIELA. Immunogenic Domains and Secondary Structure of Escherichia coli Recombinant Secreted Protein Escherichia coli-Secreted Protein B. FRONTIERS IN IMMUNOLOGY, v. 8, APR 24 2017. Web of Science Citations: 1.
OZAKI, CHRISTIANE Y.; SILVEIRA, CAIO R. F.; ANDRADE, FERNANDA B.; NEPOMUCENO, ROBERTO; SILVA, ANDERSON; MUNHOZ, DANIELLE D.; YAMAMOTO, BRUNO B.; LUZ, DANIELA; ABREU, PATRICIA A. E.; HORTON, DENISE S. P. Q.; ELIAS, WALDIR P.; RAMOS, OSCAR H. P.; PIAZZA, ROXANE M. F. Single Chain Variable Fragments Produced in Escherichia coli against Heat-Labile and Heat-Stable Toxins from Enterotoxigenic E-coli. PLoS One, v. 10, n. 7 JUL 8 2015. Web of Science Citations: 4.
LUZ, DANIELA; CHEN, GANG; MARANHAO, ANDREA Q.; ROCHA, LETICIA B.; SIDHU, SACHDEV; PIAZZA, ROXANE M. F. Development and Characterization of Recombinant Antibody Fragments That Recognize and Neutralize In Vitro Stx2 Toxin from Shiga Toxin-Producing Escherichia coli. PLoS One, v. 10, n. 3 MAR 19 2015. Web of Science Citations: 11.

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