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Identifying biomarkers for embryo biotechnologies through proteomics and lipid fingerprinting of bovine embryos

Abstract

Although currently the national livestock has reached a high technological level, certain factors affect the reproductive performance of animals. Thus, lowering productivity, profitability, and therefore the competitiveness of livestock. The biggest limitation to reproductive efficiency among mammals is embryonic mortality, estimated at 25-60%. The quality and survival of embryos produced in vitro, as well as by nuclear transfer of somatic cells are lower than those of embryos generated in vivo. Little is known of the changes in gene expression and protein and lipid profile found in pre-implantation embryos obtained from bovine in vitro fertilization and nuclear transfer of somatic cells when compared with those obtained by embryo transfer. This project proposes to investigate the role of proteins and lipids in embryo mortality and the effect of different embryo biotechnologies about this process, aimed at: 1) characterize differences in protein profile of cattle Bos taurus and Bos indicus pre-implantation embryos derived from in vitro fertilization, embryo transfer and nuclear transfer of somatic cells and systematize the information obtained in order to choose candidate proteins to carry out deeper analysis; 2) identify the proteins of interest; 3) identify possible variations in protein and lipid profile related to the sex of embryos obtained by nuclear transfer of somatic cells; 4) characterize the fatty acid composition of pre-implantation embryos; 5) identify biomarkers associated with embryonic loss. (AU)

Articles published in Pesquisa FAPESP Magazine about the research grant:
Fertilization markers 
Marcadores de la fertilización 
Articles published in Agência FAPESP Newsletter about the research grant:
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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GUERREIRO, TATIANE MELINA; GONCALVES, ROSELI FERNANDES; RODRIGUES MELO, CARLOS FERNANDO O.; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; VISINTIN, JOSE ANTONIO; DE ACHILLES, MARCOS ANTONIO; CATHARINO, RODRIGO RAMOS. A Metabolomic Overview of Follicular Fluid in Cows. FRONTIERS IN VETERINARY SCIENCE, v. 5, FEB 8 2018. Web of Science Citations: 5.
GONCALVES, ROSELI F.; FERREIRA, MONICA S.; DE OLIVEIRA, DIOGO N.; CANEVAROLO, RAFAEL; ACHILLES, MARCOS A.; D'ERCOLE, DANIELA L.; BOLS, PETER E.; VISINTIN, JOSE A.; KILLIAN, GARY J.; CATHARINO, RODRIGO R. Analysis and characterisation of bovine oocyte and embryo biomarkers by matrix-assisted desorption ionisation mass spectrometry imaging. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 28, n. 3, p. 293-301, 2016. Web of Science Citations: 5.

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