Mitochondrial energy metabolism, redox state and functionality in cell death and cardiometabolic and neurodegenerative disorders

• Grant number: 11/50400-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: October 01, 2011
• End date: September 30, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Aníbal Eugênio Vercesi
• Grantee: Aníbal Eugênio Vercesi
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Pesquisadores principais: Helena Coutinho Franco de Oliveira ; Rodrigo Ramos Catharino ; Roger Frigério Castilho
• Associated scholarship(s): 13/17837-0 - Mitochondrial activity in human glioma cells and your relationship with the Warburg effect, BP.IC; 12/18351-1 - Inhibitory effect of local anesthetics on complex I inhibition-stimulated mitochondrial reactive oxygen species production, BP.IC; 11/51800-1 - Muscle damage induced by eccentric exercise: involvement of mitochondria and interactions with statin myotoxicity, BP.PD

Abstract

In addition to be the cell’s powerhouse, mitochondria also contains a cell death machinery. Indeed, after a number of new mitochondrial proteins and modulator metabolites have been discovered, this organelle has emerged as a center of attention in processes of cell signaling, cell injury and cell death. Evidence has been provided that mitochondria comprise one of the main pathways for cell death through the opening of highly regulated membrane pores, increased rates of reactive oxygen species (ROS) production, and mitochondrial DNA mutations. High levels of matrix Ca2+ stimulate ROS production and NADPH oxidation, and lead to opening of pores in the inner mitochondrial membrane (permeability transition pore, PTP). When PTP opens in a large number of mitochondria, cell death may occur due to the lack of ATP (necrosis). In addition, mitochondrial outer membrane permeabilization (MOMP) induces cell death (apoptosis) through the release of molecules that promote both caspase-dependent and caspase-independent cell death. MOMP itself can occur via processes dependent or independent of PTP opening. Mitochondrial dysfunction has been associated with several cardiac, metabolic and neurodegenerative disorders. In these states, many signal transduction molecules, toxins or experimental drugs can act either inducing opening or closing of the mitochondrial membrane pores. Taken together, it seems that mitochondrial cell death control has wide implications in cell physiology, pathology and pharmacology. We have recently demonstrated that mitochondrially-mediated redox imbalance is an important part of the pathogenesis of genetic dyslipidemias, neurodegenerative disorders, and may control the death of tumor cells. In this project we propose to carry on further investigation on the mitochondrial involvement on these conditions, expanding the elucidation of mitochondrial mechanisms that leads to cell death using in vivo and in vitro approaches. We will also investigate the mitochondrial involvement in the pathogenesis of additional neuro and cardiometabolic disorders (secondary hyperlipidemia, diabetes, hypertension, alcoholism, Alzheimer’s disease). In addition, new “omics” approaches (metabolome, lipidome and proteome) will be used to fast disclose metabolic pathways altered in the situations where mitochondrial dysfunctions were already demonstrated by us. (AU)

Scientific publications

(76)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DORIGHELLO, GABRIEL G.; PAIM, BRUNO A.; KIIHL, SAMARA F.; FERREIRA, MONICA S.; CATHARINO, RODRIGO R.; VERCESI, ANIBAL E.; OLIVEIRA, HELENA C. F.. Correlation between Mitochondrial Reactive Oxygen and Severity of Atherosclerosis. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2016. (11/50400-0)

RONCHI, JULIANA A.; HENNING, BARBARA; RAVAGNANI, FELIPE G.; FIGUEIRA, TIAGO R.; CASTILHO, ROGER F.; DOS REIS, SERGIO F.; VERCESI, ANIBAL E.. Increased Susceptibility of Gracilinanus microtarsus Liver Mitochondria to Ca2+-Induced Permeability Transition Is Associated with a More Oxidized State of NAD(P). OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2015. (11/50400-0)

BUSANELLO, ESTELA N. B.; FIGUEIRA, TIAGO R.; MARQUES, ANA C.; NAVARRO, CLAUDIA D. C.; OLIVEIRA, HELENA C. F.; VERCESI, ANIBAL E.. Facilitation of Ca2+-induced opening of the mitochondrial permeability transition pore either by nicotinamide nucleotide transhydrogenase deficiency or statins treatment. Cell Biology International, v. 42, n. 6, SI, p. 742-746, JUN 2018. (06/59786-0, 11/50400-0, 14/11261-2, 13/07607-8)

PAIVA, ADRIENE A.; RAPOSO, HELENA F.; WANSCHEL, AMARYLIS C. B. A.; NARDELLI, TARLLIZA R.; OLIVEIRA, HELENA C. F.. Apolipoprotein CIII Overexpression-Induced Hypertriglyceridemia Increases Nonalcoholic Fatty Liver Disease in Association with Inflammation and Cell Death. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2017. (11/50400-0, 13/07607-8)

DORIGHELLO, GABRIEL G.; PAIM, BRUNO A.; LEITE, ANA CATARINA R.; VERCESI, ANIBAL E.; OLIVEIRA, HELENA C. F.. Spontaneous experimental atherosclerosis in hypercholesterolemic mice advances with ageing and correlates with mitochondrial reactive oxygen species. Experimental Gerontology, v. 109, n. SI, p. 47-50, AUG 2018. (11/50400-0, 13/07607-8)

RONCHI, JULIANA APARECIDA; FRANCISCO, ANNELISE; CORREA PASSOS, LUIZ AUGUSTO; FIGUEIRA, TIAGO REZENDE; CASTILHO, ROGER FRIGERIO. The Contribution of Nicotinamide Nucleotide Transhydrogenase to Peroxide Detoxification Is Dependent on the Respiratory State and Counterbalanced by Other Sources of NADPH in Liver Mitochondria. Journal of Biological Chemistry, v. 291, n. 38, p. 20173-20187, SEP 16 2016. (11/50400-0, 15/22063-0)

FACCHINI, GUSTAVO; IGNARRO, RAFFAELA SILVESTRE; RODRIGUES-SILVA, ERIKA; VIEIRA, ANDRE SCHWAMBACH; LOPES-CENDES, ISCIA; CASTILHO, ROGER FRIGERIO; ROGERIO, FABIO. Toxic effects of phytol and retinol on human glioblastoma cells are associated with modulation of cholesterol and fatty acid biosynthetic pathways. JOURNAL OF NEURO-ONCOLOGY, v. 136, n. 3, p. 435-443, FEB 2018. (11/50400-0, 13/07559-3, 13/02618-1)

MICHELINI, LUIZ G. B.; FIGUEIRA, TIAGO R.; SIQUEIRA-SANTOS, EDILERIE S.; CASTILHO, ROGER F.. Rotenone exerts similar stimulatory effects on H2O2 production by isolated brain mitochondria from young-adult and old rats. Neuroscience Letters, v. 589, p. 25-30, MAR 4 2015. (11/50400-0, 11/51800-1, 11/14229-4)

LUZ, LUCIANA DE ANDRADE; ROSSATO, FRANCO APARECIDO; PEREIRA E COSTA, RUTE ALVES; NAPOLEAO, THIAGO HENRIQUE; GUEDES PAIVA, PATRICIA MARIA; BREITENBACH BARROSO COELHO, LUANA CASSANDRA. Cytotoxicity of the coagulant Moringa oleifera lectin (cMoL) to B16-F10 melanoma cells. TOXICOLOGY IN VITRO, v. 44, p. 94-99, OCT 2017. (11/50400-0)

MELO, CARLOS FERNANDO O. R.; DELAFIORI, JEANY; DE OLIVEIRA, DIOGO N.; GUERREIRO, TATIANE M.; ESTEVES, CIBELE Z.; LIMA, ESTELA DE O.; PANDO-ROBLES, VICTORIA; CATHARINO, RODRIGO R.; NETWORK, ZIKA-UNICAMP. Serum Metabolic Alterations upon Zika Infection. FRONTIERS IN MICROBIOLOGY, v. 8, OCT 10 2017. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

DE OLIVEIRA, DIOGO N.; DE MENEZES, MAICO; CATHARINO, RODRIGO R.. Thermal degradation of sucralose: a combination of analytical methods to determine stability and chlorinated byproducts. SCIENTIFIC REPORTS, v. 5, APR 15 2015. (11/50400-0, 14/00084-2)

DE OLIVEIRA, DIOGO NOIN; DELAFIORI, JEANY; FERREIRA, MONICA SIQUEIRA; CATHARINO, RODRIGO RAMOS. In vitro evaluation of Sun Protection Factor and stability of commercial sunscreens using mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 988, p. 13-19, APR 15 2015. (13/20066-6, 11/50400-0, 14/00084-2)

ODIR RODRIGUES MELO, CARLOS FERNANDO; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; GUERREIRO, TATIANE MELINA; ESTEVES, CIBELE ZANARDI; BECK, RAISSA MARQUES; PADILLA, MARINA AIELLO; MILANEZ, GUILHERME PAIER; ARNS, CLARICE WEIS; PROENCA-MODENA, JOSE LUIZ; et al. A Lipidomics Approach in the Characterization of Zika-Infected Mosquito Cells: Potential Targets for Breaking the Transmission Cycle. PLoS One, v. 11, n. 10, OCT 10 2016. (15/06809-1, 14/00302-0, 11/50400-0, 14/00084-2, 13/11343-6)

MALAGUTI, C.; LA GUARDIA, P. G.; LEITE, A. C. R.; OLIVEIRA, D. N.; DE LIMA ZOLLNER, R. L.; CATHARINO, R. R.; VERCESI, A. E.; OLIVEIRA, H. C. F.. Oxidative stress and susceptibility to mitochondrial permeability transition precedes the onset of diabetes in autoimmune non-obese diabetic mice. Free Radical Research, v. 48, n. 12, p. 1494-1504, DEC 2014. (11/50400-0)

MICHELINI, LUIZ G. B.; BENEVENTO, CARLOS E.; ROSSATO, FRANCO A.; SIQUEIRA-SANTOS, EDILENE S.; CASTILHO, ROGER F.. Effects of Partial Inhibition of Respiratory Complex I on H2O2 Production by Isolated Brain Mitochondria in Different Respiratory States. Neurochemical Research, v. 39, n. 12, p. 2419-2430, DEC 2014. (11/50400-0, 11/14229-4)

HUANG, GUOZHONG; VERCESI, ANIBAL E.; DOCAMPO, ROBERTO. Essential regulation of cell bioenergetics in Trypanosoma brucei by the mitochondrial calcium uniporter. NATURE COMMUNICATIONS, v. 4, DEC 2013. (11/50400-0)

ARANDA-SOUZA, MARY A.; ROSSATO, FRANCO A.; COSTA, RUTE A. P.; FIGUEIRA, TIAGO R.; CASTILHO, ROGER F.; GUARNIERE, MIRIAM C.; NUNES, ERIKA S.; COELHO, LUANA C. B. B.; CORREIA, MARIA T. S.; VERCESI, ANIBAL E.. A lectin from Bothrops leucurus snake venom raises cytosolic calcium levels and promotes B16-F10 melanoma necrotic cell death via mitochondrial permeability transition. Toxicon, v. 82, p. 97-103, MAY 2014. (12/07424-8, 11/51800-1, 11/50400-0)

CECATTO, CRISTIANE; AMARAL, ALEXANDRE UMPIERREZ; LEIPNITZ, GUILHIAN; CASTILHO, ROGER FRIGERIO; WAJNER, MOACIR. Ethylmalonic Acid Induces Permeability Transition in Isolated Brain Mitochondria. NEUROTOXICITY RESEARCH, v. 26, n. 2, p. 168-178, AUG 2014. (11/50400-0)

ROSSATO, FRANCO A.; ZECCHIN, KARINA G.; LA GUARDIA, PAOLO G.; ORTEGA, ROSE M.; ALBERICI, LUCIANE C.; COSTA, RUTE A. P.; CATHARINO, RODRIGO R.; GRANER, EDGARD; CASTILHO, ROGER F.; VERCESI, ANIBAL E.. Fatty Acid Synthase Inhibitors Induce Apoptosis in Non-Tumorigenic Melan-A Cells Associated with Inhibition of Mitochondrial Respiration. PLoS One, v. 9, n. 6, JUN 25 2014. (06/59786-0, 11/50400-0)

ESTEVES, CIBELE ZANARDI; DIAS, LETICIA DE AGUIAR; LIMA, ESTELA DE OLIVEIRA; DE OLIVEIRA, DIOGO NOIN; ODIR RODRIGUES MELO, CARLOS FERNANDO; DELAFIORI, JEANY; SOUZA GOMEZ, CARLA CRISTINA; RIBEIRO, JOSE DIRCEU; RIBEIRO, ANTONIO FERNANDO; LEVY, CARLOS EMILIO; et al. Skin Biomarkers for Cystic Fibrosis: A Potential Non-Invasive Approach for Patient Screening. FRONTIERS IN PEDIATRICS, v. 5, JAN 10 2018. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

GODOY FALCO, LUIS FERNANDO; ODIR RODRIGUES MELO, CARLOS FERNANDO; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; CATHARINO, RODRIGO RAMOS. An LDI-MSI approach for targeted and untargeted differentiation and assessment of pharmaceutical formulations. Talanta, v. 197, p. 92-97, MAY 15 2019. (11/50400-0, 15/06809-1, 16/17066-2)

LORZA-GIL, ESTELA; DE SOUZA, JANE C.; GARCIA-AREVALO, MARTA; VETTORAZZI, JEAN F.; MARQUES, ANA CAROLINA; SALERNO, ALESSANDRO G.; TRIGO, JOSE ROBERTO; OLIVEIRA, HELENA C. F.. Coenzyme Q(10) protects against -cell toxicity induced by pravastatin treatment of hypercholesterolemia. Journal of Cellular Physiology, v. 234, n. 7, p. 11047-11059, JUL 2019. (11/50400-0, 13/07607-8)

LIMA, ESTELA DE O.; GUERREIRO, TATIANE M.; MELO, CARLOS FERNANDO O. R.; DE OLIVEIRA, DIOGO N.; MACHADO, DAISY; LANCELLOTI, MARCELO; CATHARINO, RODRIGO R.. MALDI imaging detects endogenous digoxin in glioblastoma cells infected by Zika virusWould it be the oncolytic key?. Journal of Mass Spectrometry, v. 53, n. 3, p. 257-263, MAR 2018. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

RUAS, JULIANA S.; SIQUEIRA-SANTOS, EDILENE S.; RODRIGUES-SILVA, ERIKA; CASTILHO, ROGER F.. High glycolytic activity of tumor cells leads to underestimation of electron transport system capacity when mitochondrial ATP synthase is inhibited. SCIENTIFIC REPORTS, v. 8, NOV 26 2018. (17/17728-8, 11/50400-0)

DABAJA, MOHAMED ZIAD; LIMA, ESTELA DE OLIVEIRA; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; ODIR RODRIGUES MELO, CARLOS FERNANDO; MORISHITA, KAREN NODA; LANCELLOTTI, MARCELO; TASCA GOIS RUIZ, ANA LUCIA; GOULART, GISELE; DUARTE, DIEGO ANDREAZZI; et al. Metabolic alterations induced by attenuated Zika virus in glioblastoma cells. CELL AND BIOSCIENCE, v. 8, SEP 4 2018. (15/06809-1, 11/50400-0, 16/17066-2, 13/07607-8)

BUSANELLO, ESTELA N. B.; MARQUES, ANA C.; LANDER, NOELIA; DE OLIVEIRA, DIOGO N.; CATHARINO, RODRIGO R.; OLIVEIRA, HELENA C. F.; VERCESI, ANIBAL E.. Pravastatin Chronic Treatment Sensitizes Hypercholesterolemic Mice Muscle to Mitochondrial Permeability Transition: Protection by Creatine or Coenzyme Q(10). FRONTIERS IN PHARMACOLOGY, v. 8, APR 5 2017. (14/00084-2, 14/11261-2, 14/08995-4, 13/07607-8, 11/50400-0)

DE OLIVEIRA, DIOGO N.; LIMA, ESTELA O.; MELO, CARLOS F. O. R.; DELAFIORI, JEANY; GUERREIRO, TATIANE M.; RODRIGUES, RAFAEL G. M.; MORISHITA, KAREN N.; SILVEIRA, CYNTHIA; MURARO, STEFANIE PRIMON; DE SOUZA, GABRIELA FABIANO; et al. Inflammation markers in the saliva of infants born from Zika-infected mothers: exploring potential mechanisms of microcephaly during fetal development. SCIENTIFIC REPORTS, v. 9, SEP 20 2019. (18/03321-6, 11/50400-0, 13/07607-8, 16/17066-2)

AMARAL, ALEXANDRE UMPIERREZ; CECATTO, CRISTIANE; CASTILHO, ROGER FRIGERIO; WAJNER, MOACIR. 2-Methylcitric acid impairs glutamate metabolism and induces permeability transition in brain mitochondria. Journal of Neurochemistry, v. 137, n. 1, p. 62-75, APR 2016. (11/50400-0)

NAVARRO, CLAUDIA D. C.; FIGUEIRA, TIAGO R.; FRANCISCO, ANNELISE; DAL'BO, GENOEFA A.; RONCHI, JULIANA A.; ROVANI, JULIANA C.; ESCANHOELA, CECILIA A. F.; OLIVEIRA, HELENA C. F.; CASTILHO, ROGER F.; VERCESI, ANIBAL E.. Redox imbalance due to the loss of mitochondrial NAD(P)-transhydrogenase markedly aggravates high fat diet-induced fatty liver disease in mice. Free Radical Biology and Medicine, v. 113, p. 190-202, DEC 2017. (14/02819-0, 11/50400-0, 15/22063-0, 13/07607-8)

CASQUERO, ANDREA CAMARGO; BERTI, JAIRO AUGUSTO; SAMPAIO TEIXEIRA, LAURA LAUAND; FRANCO DE OLIVEIRA, HELENA COUTINHO. Chronic Exercise Reduces CETP and Mesterolone Treatment Counteracts Exercise Benefits on Plasma Lipoproteins Profile: Studies in Transgenic Mice. LIPIDS, v. 52, n. 12, p. 981-990, DEC 2017. (11/50400-0)

FIGUEIROA, EVELLYNE DE OLIVEIRA; ARANDA-SOUZA, MARY ANGELA; VAREJAO, NATHALIA; ROSSATO, FRANCO APARECIDO; PEREIRA COSTA, RUTE ALVES; FIGUEIRA, TIAGO REZENDE; NASCIMENTO DA SILVA, LUIS CLAUDIO; CASTILHO, ROGER FRIGERIO; VERCESI, ANIBAL EUGENIO; DOS SANTOS CORREIA, MARIA TEREZA. pCramoll and rCramoll lectins induce cell death in human prostate adenocarcinoma (PC-3) cells by impairment of mitochondrial homeostasis. TOXICOLOGY IN VITRO, v. 43, p. 40-46, SEP 2017. (11/50400-0, 11/51800-1)

ODIR RODRIGUES MELO, CARLOS FERNANDO; BACHUR, LUIS FELIPE; DELAFIORI, JEANY; DABAJA, MOHAMED ZIAD; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; TARARAM, CIBELE APARECIDA; BUSSO-LOPES, ARIANE FIDELIS; MORETTI, MARIA LUIZA; CATHARINO, RODRIGO RAMOS. Does leukotriene F4 play a major role in the infection mechanism of Candida sp.?. Microbial Pathogenesis, v. 149, DEC 2020. (16/17066-2, 11/50400-0, 18/03321-6, 15/06809-1)

ODIR RODRIGUES MELO, CARLOS FERNANDO; ESTEVES, CIBELE ZANARDI; DE OLIVEIRA, ROSIMEIRE NUNES; GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; MINE, JULIO CESAR; ALLEGRETTI, SILMARA MARQUES; CATHARINO, RODRIGO RAMOS. Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry. Parasitology Research, v. 115, n. 11, p. 8-pg., 2016-11-01. (14/00084-2, 14/00302-0, 11/50400-0, 15/06809-1)

SALERNO, ALESSANDRO G.; RENTZ, THIAGO; DORIGHELLO, GABRIEL G.; MARQUES, ANA CAROLINA; LORZA-GIL, ESTELA; WANSCHEL, AMARYLIS C. B. A.; DE MORAES, AUDREY; VERCESI, ANIBAL E.; OLIVEIRA, HELENA C. F.. Lack of mitochondrial NADP(H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice. Biochemical Journal, v. 476, p. 21-pg., 2019-12-01. (11/15103-4, 11/15101-1, 13/05497-0, 11/50400-0, 17/02903-9, 13/07607-8, 17/17728-8)

DORIGHELLO, GABRIEL G.; PAIM, BRUNO A.; KIIHL, SAMARA F.; FERREIRA, MONICA S.; CATHARINO, RODRIGO R.; VERCESI, ANIBAL E.; OLIVEIRA, HELENA C. F.. Correlation between Mitochondrial Reactive Oxygen and Severity of Atherosclerosis. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2016, p. 10-pg., 2016-01-01. (11/50400-0)

BUSANELLO, ESTELA N. B.; FIGUEIRA, TIAGO R.; MARQUES, ANA C.; NAVARRO, CLAUDIA D. C.; OLIVEIRA, HELENA C. F.; VERCESI, ANIBAL E.. Facilitation of Ca2+-induced opening of the mitochondrial permeability transition pore either by nicotinamide nucleotide transhydrogenase deficiency or statins treatment. Cell Biology International, v. 42, n. 6, p. 5-pg., 2018-06-01. (11/50400-0, 06/59786-0, 14/11261-2, 13/07607-8)

ODIR RODRIGUES MELO, CARLOS FERNANDO; ESTEVES, CIBELE ZANARDI; DE OLIVEIRA, ROSIMEIRE NUNES; GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; MINE, JULIO CESAR; ALLEGRETTI, SILMARA MARQUES; CATHARINO, RODRIGO RAMOS. Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry. Parasitology Research, v. 115, n. 11, p. 4107-4114, NOV 2016. (14/00084-2, 15/06809-1, 14/00302-0, 11/50400-0)

DORIGHELLO, GABRIEL G.; INADA, NATALIA M.; PAIM, BRUNO A.; PARDO-ANDREU, GILBERTO L.; VERCESI, ANIBAL E.; OLIVEIRA, HELENA C. F.. Mangifera indica L. extract (Vimang (R)) reduces plasma and liver cholesterol and leucocyte oxidative stress in hypercholesterolemic LDL receptor deficient mice. Cell Biology International, v. 42, n. 6, SI, p. 747-753, JUN 2018. (13/07607-8, 11/50400-0)

SALERNO, ALESSANDRO G.; RENTZ, THIAGO; DORIGHELLO, GABRIEL G.; MARQUES, ANA CAROLINA; LORZA-GIL, ESTELA; WANSCHEL, AMARYLIS C. B. A.; DE MORAES, AUDREY; VERCESI, ANIBAL E.; OLIVEIRA, HELENA C. F.. Lack of mitochondrial NADP(H)-transhydrogenase expression in macrophages exacerbates atherosclerosis in hypercholesterolemic mice. Biochemical Journal, v. 476, n. 24, p. 3769-3789, DEC 2019. (11/15101-1, 11/15103-4, 11/50400-0, 13/05497-0, 17/17728-8, 13/07607-8, 17/02903-9)

ALBERICI, LUCIANE C.; PAIM, BRUNO A.; ZECCHIN, KARINA G.; MIRANDOLA, SANDRA R.; PESTANA, CEZAR R.; CASTILHO, ROGER F.; VERCESI, ANIBAL E.; OLIVEIRA, HELENA C. F.. Activation of the mitochondrial ATP-sensitive K+ channel reduces apoptosis of spleen mononuclear cells induced by hyperlipidemia. LIPIDS IN HEALTH AND DISEASE, v. 12, JUN 14 2013. (06/59786-0, 11/50400-0)

DE OLIVEIRA, DIOGO N.; CAMARGO, ANA C. B.; MELO, CARLOS F. O. R.; CATHARINO, RODRIGO R.. A fast semi-quantitative screening for cocoa content in chocolates using MALDI-MSI. Food Research International, v. 103, p. 8-11, JAN 2018. (14/00084-2, 14/21791-9, 11/50400-0, 15/06809-1, 16/17066-2)

RODRIGUES-SILVA, ERIKA; SIQUEIRA-SANTOS, EDILENE S.; RUAS, JULIANA S.; IGNARRO, RAFFAELA S.; FIGUEIRA, TIAGO R.; ROGERIO, FABIO; CASTILHO, ROGER F.. Evaluation of mitochondrial respiratory function in highly glycolytic glioma cells reveals low ADP phosphorylation in relation to oxidative capacity. JOURNAL OF NEURO-ONCOLOGY, v. 133, n. 3, p. 519-529, JUL 2017. (11/50400-0, 13/02618-1)

GUERREIRO, TATIANE MELINA; GONCALVES, ROSELI FERNANDES; RODRIGUES MELO, CARLOS FERNANDO O.; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; VISINTIN, JOSE ANTONIO; DE ACHILLES, MARCOS ANTONIO; CATHARINO, RODRIGO RAMOS. A Metabolomic Overview of Follicular Fluid in Cows. FRONTIERS IN VETERINARY SCIENCE, v. 5, FEB 8 2018. (15/06809-1, 11/18085-7, 11/50400-0, 16/17066-2, 10/01077-9)

ODIR RODRIGUES MELO, CARLOS FERNANDO; NAVARRO, LUIZ CLAUDIO; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; LIMA, ESTELA DE OLIVEIRA; DELAFIORI, JEANY; DABAJA, MOHAMED ZIAD; RIBEIRO, MARTA DA SILVA; DE MENEZES, MAICO; MARTINS RODRIGUES, RAFAEL GUSTAVO; et al. A Machine Learning Application Based in Random Forest for Integrating Mass Spectrometry-Based Metabolomic Data: A Simple Screening Method for Patients With Zika Virus. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 6, APR 11 2018. (14/00302-0, 11/50400-0, 15/06809-1, 16/17066-2, 17/12646-3)

CHWEIH, HANAN; CASTILHO, ROGER F.; FIGUEIRA, TIAGO R.. Tissue and sex specificities in Ca2+ handling by isolated mitochondria in conditions avoiding the permeability transition. Experimental Physiology, v. 100, n. 9, p. 1073-1092, SEP 1 2015. (11/50400-0, 11/51800-1)

FERNANDES, CAROLINA GONCALVES; PIEROZAN, PAULA; SOARES, GILBERTO MACHADO; FERREIRA, FERNANDA; ZANATTA, ANGELA; AMARAL, ALEXANDRE UMPIERREZ; BORGES, CLARISSA GUENTHER; WAJNER, MOACIR; PESSOA-PUREUR, REGINA. NMDA Receptors and Oxidative Stress Induced by the Major Metabolites Accumulating in HMG Lyase Deficiency Mediate Hypophosphorylation of Cytoskeletal Proteins in Brain From Adolescent Rats: Potential Mechanisms Contributing to the Neuropathology of This Disease. NEUROTOXICITY RESEARCH, v. 28, n. 3, p. 239-252, OCT 2015. (11/50400-0)

DOCAMPO, ROBERTO; VERCESI, ANIBAL E.; HUANG, GUOZHONG. Mitochondrial calcium transport in trypanosomes. Molecular and Biochemical Parasitology, v. 196, n. 2, p. 108-116, SEP 2014. (11/50400-0, 13/50624-0)

PADILHA MENDONCA, MONIQUE CULTURATO; SOARES, EDILENE SIQUEIRA; DE JESUS, MARCELO BISPO; CERAGIOLI, HELDER JOSE; FERREIRA, MONICA SIQUEIRA; CATHARINO, RODRIGO RAMOS; DA CRUZ-HOEFLING, MARIA ALICE. Reduced graphene oxide induces transient blood-brain barrier opening: an in vivo study. JOURNAL OF NANOBIOTECHNOLOGY, v. 13, OCT 30 2015. (11/50400-0, 12/24782-5)

IGNARRO, RAFFAELA SILVESTRE; FACCHINI, GUSTAVO; VIEIRA, ANDRE SCHWAMBACH; DE MELO, DANIELA RODRIGUES; LOPES-CENDES, ISCIA; CASTILHO, ROGER FRIGERIO; ROGERIO, FABIO. Sulfasalazine intensifies temozolomide cytotoxicity in human glioblastoma cells. Molecular and Cellular Biochemistry, v. 418, n. 1-2, p. 167-178, JUL 2016. (11/50400-0, 13/07559-3, 13/02618-1)

DOS SANTOS, GUSTAVO APARECIDO; FERREIRA, MONICA SIQUEIRA; DE OLIVEIRA, DIOGO NOIN; DE OLIVEIRA, VANESSA; SIQUEIRA-SANTOS, EDILENE S.; CORREA CINTRA, DENNYS ESPER; CASTILHO, ROGER FRIGERIO; VELLOSO, LICIO AUGUSTO; CATHARINO, RODRIGO RAMOS. Identification of compounds from high-fat and extra virgin olive oil-supplemented diets in whole mouse liver extracts and isolated mitochondria using mass spectrometry. Journal of Mass Spectrometry, v. 50, n. 7, p. 951-958, JUL 2015. (11/50400-0, 14/00084-2)

EHX, GREGORY; GERIN, STEPHANIE; MATHY, GREGORY; FRANCK, FABRICE; OLIVEIRA, HELENA C. F.; VERCESI, ANIBAL E.; SLUSE, FRANCIS E.. Liver proteomic response to hypertriglyceridemia in human-apolipoprotein C-III transgenic mice at cellular and mitochondrial compartment levels. LIPIDS IN HEALTH AND DISEASE, v. 13, JUL 21 2014. (11/50400-0)

GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; FERREIRA, MONICA SIQUEIRA; CATHARINO, RODRIGO RAMOS. High-throughput analysis by SP-LDI-MS for fast identification of adulterations in commercial balsamic vinegars. Analytica Chimica Acta, v. 838, p. 86-92, AUG 1 2014. (11/50400-0, 14/00084-2)

LORZA-GIL, ESTELA; SALERNO, ALESSANDRO G.; WANSCHEL, AMARYLIS C. B. A.; VETTORAZZI, JEAN F.; FERREIRA, MONICA S.; RENTZ, THIAGO; CATHARINO, RODRIGO R.; OLIVEIRA, HELENA C. F.. Chronic use of pravastatin reduces insulin exocytosis and increases beta-cell death in hypercholesterolemic mice. Toxicology, v. 344, p. 42-52, FEB 17 2016. (11/50400-0)

MORI, MATEUS P.; COSTA, RUTE A. P.; SOLTYS, DANIELA T.; FREIRE, THIAGO DE S.; ROSSATO, FRANCO A.; AMIGO, IGNACIO; KOWALTOWSKI, ALICIA J.; VERCESI, ANBAL E.; DE SOUZA-PINTO, NADJA C.. Lack of XPC leads to a shift between respiratory complexes I and II but sensitizes cells to mitochondrial stress. SCIENTIFIC REPORTS, v. 7, MAR 13 2017. (12/11889-6, 08/57952-5, 10/51906-1, 11/50400-0, 08/57721-3, 06/59786-0, 13/07937-8)

DE OLIVEIRA, DIOGO N.; FERREIRA, MONICA S.; CATHARINO, RODRIGO R.. Rapid and Simultaneous In Situ Assessment of Aflatoxins and Stilbenes Using Silica Plate Imprinting Mass Spectrometry Imaging. PLoS One, v. 9, n. 3, MAR 4 2014. (11/50400-0)

GUERREIRO, TATIANE MELINA; OZAWA, KUMI SHIOTA; LIMA, ESTELA DE OLIVEIRA; ODIR RODRIGUES MELO, CARLOS FERNANDO; DE OLIVEIRA, DIOGO NOIN; DO NASCIMENTO TRIANO, SIMONE PEREIRA; CATHARINO, RODRIGO RAMOS. New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachacas. FRONTIERS IN NUTRITION, v. 5, APR 6 2018. (11/50400-0, 15/06809-1)

FERNANDES, CAROLINA GONCALVES; NUNES RODRIGUES, MARILIA DANYELLE; SEMINOTTI, BIANCA; LAURA COLIN-GONZALEZ, ANA; SANTAMARIA, ABEL; QUINCOZES-SANTOS, ANDRE; WAJNER, MOACIR. Induction of a Proinflammatory Response in Cortical Astrocytes by the Major Metabolites Accumulating in HMG-CoA Lyase Deficiency: the Role of ERK Signaling Pathway in Cytokine Release. Molecular Neurobiology, v. 53, n. 6, p. 3586-3595, AUG 2016. (11/50400-0)

FERNANDES, ANNA MARIA A. P.; VENDRAMINI, PEDRO H.; GALAVERNA, RENAN; SCHWAB, NICOLAS V.; ALBERICI, LUCIANE C.; AUGUSTI, RODINEI; CASTILHO, ROGER F.; EBERLIN, MARCOS N.. Direct Visualization of Neurotransmitters in Rat Brain Slices by Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI - MS). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, v. 27, n. 12, p. 1944-1951, DEC 2016. (10/51677-2, 11/50400-0)

DE MENEZES, MAICO; DE OLIVEIRA, DIOGO NOIN; CATHARINO, RODRIGO RAMOS. Capillary-induced Homogenization of Matrix in Paper: A Powerful Approach for the Quantification of Active Pharmaceutical Ingredients Using Mass Spectrometry Imaging. SCIENTIFIC REPORTS, v. 6, JUL 21 2016. (15/06809-1, 11/50400-0, 14/00084-2)

FERREIRA, MONICA SIQUEIRA; DE OLIVEIRA, ROSIMEIRE NUNES; DE OLIVEIRA, DIOGO NOIN; ESTEVES, CIBELE ZANARDI; ALLEGRETTI, SILMARA MARQUES; CATHARINO, RODRIGO RAMOS. Revealing praziquantel molecular targets using mass spectrometry imaging: an expeditious approach applied to Schistosoma mansoni. International Journal for Parasitology, v. 45, n. 6, p. 385-391, MAY 2015. (14/00302-0, 11/50400-0)

HICKMANN, FERNANDA HERMES; CECATTO, CRISTIANE; KLEEMANN, DANIELE; MONTEIRO, WAGNER OLIVEIRA; CASTILHO, ROGER FRIGERIO; AMARAL, ALEXANDRE UMPIERREZ; WAJNER, MOACIR. Uncoupling, metabolic inhibition and induction of mitochondrial permeability transition in rat liver mitochondria caused by the major long-chain hydroxyl monocarboxylic fatty acids accumulating in LCHAD deficiency. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, v. 1847, n. 6-7, p. 620-628, JUN-JUL 2015. (11/50400-0)

DE OLIVEIRA, D. N.; CATHARINO, R. R.. Direct metabolic fingerprinting of olive oils using STELDI-MS. Journal of Food Composition and Analysis, v. 38, p. 131-134, MAR 2015. (11/50400-0, 14/00084-2)

RAPOSO, HELENA F.; PAIVA, ADRIENE A.; KATO, LARISSA S.; DE OLIVEIRA, HELENA C. F.. Apolipoprotein CIII overexpression exacerbates diet-induced obesity due to adipose tissue higher exogenous lipid uptake and retention and lower lipolysis rates. NUTRITION & METABOLISM, v. 12, DEC 23 2015. (11/50400-0)

RAPOSO, HELENA F.; VANZELA, EMERIELLE C.; BERTI, JAIRO A.; OLIVEIRA, HELENA C. F.. Cholesteryl Ester Transfer Protein (CETP) expression does not affect glucose homeostasis and insulin secretion: studies in human CETP transgenic mice. LIPIDS IN HEALTH AND DISEASE, v. 15, JAN 13 2016. (11/50400-0)

DAMARIO, NATALIA; DE OLIVEIRA, DIOGO N.; FERREIRA, MONICA S.; DELAFIORI, JEANY; CATHARINO, RODRIGO R.. Cheese lipid profile using direct imprinting in glass surface mass spectrometry. ANALYTICAL METHODS, v. 7, n. 6, p. 2877-2880, 2015. (11/50400-0, 14/00084-2)

WANSCHEL, AMARYLIS C. B. A.; GUIZONI, DANIELE M.; LORZA-GIL, ESTELA; SALERNO, ALESSANDRO G.; PAIVA, ADRIENE A.; DORIGHELLO, GABRIEL G.; DAVEL, ANA PAULA; BALKAN, WAYNE; HARE, JOSHUA M.; OLIVEIRA, HELENA C. F.. The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction. BIOMOLECULES, v. 11, n. 1, JAN 2021. (16/01746-4, 13/05497-0, 11/50400-0, 13/07607-8, 13/09347-3, 16/01044-0)

DORIGHELLO, GABRIEL G.; INADA, NATALIA M.; PAIM, BRUNO A.; PARDO-ANDREU, GILBERTO L.; VERCESI, ANIBAL E.; OLIVEIRA, HELENA C. F.. Mangifera indica L. extract (Vimang (R)) reduces plasma and liver cholesterol and leucocyte oxidative stress in hypercholesterolemic LDL receptor deficient mice. Cell Biology International, v. 42, n. 6, p. 7-pg., 2018-06-01. (13/07607-8, 11/50400-0)

DORIGHELLO, GABRIEL G.; PAIM, BRUNO A.; LEITE, ANA CATARINA R.; VERCESI, ANIBAL E.; OLIVEIRA, HELENA C. F.. Spontaneous experimental atherosclerosis in hypercholesterolemic mice advances with ageing and correlates with mitochondrial reactive oxygen species. Experimental Gerontology, v. 109, p. 4-pg., 2018-08-01. (13/07607-8, 11/50400-0)

PAIVA, ADRIENE A.; RAPOSO, HELENA F.; WANSCHEL, AMARYLIS C. B. A.; NARDELLI, TARLLIZA R.; OLIVEIRA, HELENA C. F.. Apolipoprotein CIII Overexpression-Induced Hypertriglyceridemia Increases Nonalcoholic Fatty Liver Disease in Association with Inflammation and Cell Death. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2017, p. 18-pg., 2017-01-01. (13/07607-8, 11/50400-0)

RONCHI, JULIANA A.; HENNING, BARBARA; RAVAGNANI, FELIPE G.; FIGUEIRA, TIAGO R.; CASTILHO, ROGER F.; DOS REIS, SERGIO F.; VERCESI, ANIBAL E.. Increased Susceptibility of Gracilinanus microtarsus Liver Mitochondria to Ca2+-Induced Permeability Transition Is Associated with a More Oxidized State of NAD(P). OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2015, p. 10-pg., 2015-01-01. (11/50400-0)

VERCESI, ANIBAL E.; CASTILHO, ROGER F.; KOWALTOWSKI, ALICIA J.; DE OLIVEIRA, HELENA C. F.; DE SOUZA-PINTO, NADJA C.; FIGUEIRA, TIAGO R.; BUSANELLO, ESTELA N. B.. Mitochondrial calcium transport and the redox nature of the calcium-induced membrane permeability transition. Free Radical Biology and Medicine, v. 129, p. 1-24, DEC 2018. (17/17728-8, 11/50400-0, 06/59786-0, 14/11261-2, 13/07607-8)

MARQUES, ANA C.; BUSANELLO, ESTELA N. B.; DE OLIVEIRA, DIOGO N.; CATHARINO, RODRIGO R.; OLIVEIRA, HELENA C. F.; VERCESI, ANIBAL E.. Coenzyme Q10 or Creatine Counteract Pravastatin-Induced Liver Redox Changes in Hypercholesterolemic Mice. FRONTIERS IN PHARMACOLOGY, v. 9, JUN 27 2018. (11/50400-0, 17/17728-8, 13/07607-8)

RUAS, JULIANA S.; SIQUEIRA-SANTOS, EDILENE S.; AMIGO, IGNACIO; RODRIGUES-SILVA, ERIKA; KOWALTOWSKI, ALICIA J.; CASTILHO, ROGER F.. Underestimation of the Maximal Capacity of the Mitochondrial Electron Transport System in Oligomycin-Treated Cells. PLoS One, v. 11, n. 3, MAR 7 2016. (12/51288-1, 10/51906-1, 11/50400-0, 13/07937-8)

FERREIRA, MONICA SIQUEIRA; DE OLIVEIRA, DIOGO NOIN; MESQUITA, CAROLINE COSTA; DE LIMA BARBOSA, ANA PAULA; ANHE, GABRIEL FORATO; CATHARINO, RODRIGO RAMOS. MALDI-MSI: a fast and reliable method for direct melatonin quantification in biological fluids. JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY, v. 7, 2016. (14/00084-2, 11/50400-0, 15/06809-1)

ODIR RODRIGUES MELO, CARLOS FERNANDO; DELAFIORI, JEANY; DABAJA, MOHAMAD ZIAD; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; COLOMBO, TATIANA ELIAS; NOGUEIRA, MAURICIO LACERDA; PROENCA-MODENA, JOSE LUIZ; CATHARINO, RODRIGO RAMOS. The role of lipids in the inception, maintenance and complications of dengue virus infection. SCIENTIFIC REPORTS, v. 8, AUG 7 2018. (15/06809-1, 11/50400-0, 13/21719-3, 16/17066-2)

IGNARRO, RAFFAELA SILVESTRE; FACCHINI, GUSTAVO; DE MELO, DANIELA RODRIGUES; PELIZZARO-ROCHA, KARIN JULIANE; FERREIRA, CARMEN VERISSIMA; CASTILHO, ROGER FRIGERIO; ROGERIO, FABIO. Characteristics of sulfasalazine-induced cytotoxicity in C6 rat glioma cells. Neuroscience Letters, v. 638, p. 189-195, JAN 18 2017. (11/50400-0, 13/02618-1, 13/07559-3)

Filed patent(s) as a result of this research project