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Influence of obesity on the progression and severity of experimental induced periodontal disease in rats: histomorphometric and immunohistochemistry

Grant number: 11/08053-0
Support type:Regular Research Grants
Duration: October 01, 2011 - September 30, 2013
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Elizangela Cruvinel Zuza
Grantee:Elizangela Cruvinel Zuza
Home Institution: Centro Universitário da Fundação Educacional de Barretos (UNIFEB). Fundação Educacional de Barretos (FEBA). Barretos , SP, Brazil
Assoc. researchers:Juliana Rico Pires ; Letícia Helena Theodoro ; Valdir Gouveia Garcia

Abstract

Several studies have found an association between increased body weight and increased severity of periodontal disease, by mechanisms not well understood. The aim of this study is to evaluate the influence of the condition of obesity on the severity of periodontal disease in obese rats with experimental periodontitis. It will be used 48 Wistar rats, which are randomly distributed in two groups of 24 animals each: group I (control) - animals systemically healthy, group II (test) - animals with induced obesity. Obesity is induced by a period of approximately 120 days using high calorie diet, then there will be the ligature induction of periodontitis, considered as baseline. Periodontal disease is induced in the left lower first molar, keeping the counter-side as controls. The sacrifice periods are 7, 15 and 30 days after the baseline survey, the mandibles will be removed for laboratory processing. It will be performed histological and histometric analyses of tissue characteristics and bone loss in the furcation area, and immunohistochemical analysis using immunomarkers of bone metabolism for the identification of tartrate resistant acid phosphatase (TRAP), the ligand receptor activator of nuclear factor kappa beta (RANKL) and osteoprotegerin (OPG). Statistical analysis will be performed considering the different groups and periods by ANOVA and the statistical difference is considered significant when p <0.05. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ZUZA, ELIZANGELA PARTATA; GARCIA, VALDIR GOUVEIA; THEODORO, LETICIA HELENA; ERVOLINO, EDILSON; VELOSO FAVERO, LUIZ FERNANDO; LONGO, MARIELLEN; RIBEIRO, FERNANDO SALIMON; MARTINS, ALEX TADEU; SPOLIDORIO, LUIS CARLOS; SAMPAIO ZUANON, JOSE ANTONIO; CORREA DE TOLEDO, BENEDICTO EGBERT; PIRES, JULIANA RICO. Influence of obesity on experimental periodontitis in rats: histopathological, histometric and immunohistochemical study. CLINICAL ORAL INVESTIGATIONS, v. 22, n. 3, p. 1197-1208, APR 2018. Web of Science Citations: 6.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.