Effects of genetic induction and reprogramming through nuclear transfer in in vitro bovine embryo production

• Grant number: 11/08376-4
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2011
• End date: September 30, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Flávio Vieira Meirelles
• Grantee: Flávio Vieira Meirelles
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated researchers: Fabiana Fernandes Bressan ; Felipe Perecin ; Lawrence Charles Smith

Abstract

Strategies such as nuclear transfer and induced reprogramming have been employed in order to induce somatic cells to an embryonic-like pluripotent state. The process of nuclear reprogramming is highly desirable and has significant contributions in the study of both basic and applied science, for example, increasing the efficiency of animal production biotechnologies and medicine by enabling the autologous cell therapy in order to treat numerous diseases. More detailed and studies, however, are still required to enable such applications, since the principals of such techniques are still not fully elucidated. Therefore, specific studies on the cellular and molecular mechanisms involved in reprogramming are essential to a better understanding of these processes and also to characterize and improve their efficiency. This proposal aims to study the nuclear reprogramming induced by nuclear transfer of somatic cells, by the expression of pluripotency factors and also trough the combination of both techniques in cattle. In summary, we will analyze the expression of genes known to be essential for pluripotency, epigenetic modifications related to these genes (DNA methylation), as well as the capacity of in vitro and in vivo embryonic development. The results of this proposal will provide important answers on the molecular mechanisms involved in nuclear reprogramming. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MEIRELLES, FLAVIO VIEIRA; BRESSAN, FABIANA FERNANDES; SMITH, LAWRENCE CHARLES; PERECIN, FELIPE; CHIARATTI, MARCOS ROBERTO; STERMAN FERRAZ, JOSE BENTO. Cytoplasmatic inheritance, epigenetics and reprogramming DNA as tools in animal breeding. LIVESTOCK SCIENCE, v. 166, n. SI, p. 199-205, AUG 2014. (12/50231-6, 11/08376-4, 10/09561-7)

BRESSAN, FABIANA FERNANDES; BASSANEZZE, VINICIUS; DE FIGUEIREDO PESSOA, LAIS VICARI; SACRAMENTO, CHESTER BITTENCOURT; MALTA, TATHIANE MAISTRO; KASHIMA, SIMONE; FANTINATO NETO, PAULO; STREFEZZI, RICARDO DE FRANCISCO; GODOY PIERI, NAIRA CAROLINE; KRIEGER, JOSE EDUARDO; et al. Generation of induced pluripotent stem cells from large domestic animals. STEM CELL RESEARCH & THERAPY, v. 11, n. 1, JUN 25 2020. (15/26818-5, 11/08376-4, 09/11631-6, 13/13686-8, 13/08135-2)

PESSOA, L. V. F.; BRESSAN, F. F.; CHIARATTI, M. R.; PIRES, P. R. L.; PERECIN, F.; SMITH, L. C.; MEIRELLES, F. V.. Mitochondrial DNA dynamics during in vitro culture and pluripotency induction of a bovine Rho0 cell line. Genetics and Molecular Research, v. 14, n. 4, p. 14093-14104, 2015. (13/08135-2, 11/08376-4, 10/13384-3)

MEIRELLES, FLAVIO VIEIRA; BRESSAN, FABIANA FERNANDES; SMITH, LAWRENCE CHARLES; PERECIN, FELIPE; CHIARATTI, MARCOS ROBERTO; STERMAN FERRAZ, JOSE BENTO. Cytoplasmatic inheritance, epigenetics and reprogramming DNA as tools in animal breeding. LIVESTOCK SCIENCE, v. 166, p. 7-pg., 2014-08-01. (11/08376-4, 12/50231-6, 10/09561-7)

BRESSAN, FABIANA F.; SANGALLI, JULIANO R.; PESSA, LAIS V. F.; PIRES, PEDRO R. L.; MEIRELLES, FLAVIO V.. Insights on bovine genetic engineering and cloning. Pesquisa Veterinária Brasileira, v. 33, p. 6-pg., 2013-12-01. (08/57877-3, 11/08376-4, 09/11631-6, 08/00102-0)

BRESSAN, FABIANA F.; SANGALLI, JULIANO R.; PESSA, LAIS V. F.; PIRES, PEDRO R. L.; MEIRELLES, FLAVIO V.. Experiências em clonagem e transgenia em bovinos. Pesquisa Veterinária Brasileira, v. 33, n. 1, p. 113-118, DEC 2013. (08/57877-3, 11/08376-4, 09/11631-6, 08/00102-0)