Effects of chronic supplementation with resveratrol in a group of Military Police Firefighters undergo a physical fitness test: a double blind study

• Grant number: 11/12728-3
• Support Opportunities: Regular Research Grants
• Duration: October 01, 2011 - September 30, 2013
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Rosemari Otton
• Grantee: Rosemari Otton
• Host Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil

Abstract

Both aerobic and anaerobic exercise potentially stimulate the production of reactive oxygen species (ROS), which may or not lead to a state of oxidative stress. Most exercise protocols in the literature include sessions of acute exercise on treadmill and cycle ergometer. Several studies have investigated a variety of antioxidant supplementation in an effort to mitigate and/or suppress oxidative damage induced by exercise and maybe modulate enzymatic and non-enzymatic antioxidant defenses. While antioxidant supplementation during exercise involving vitamins C and E has been the subject of frequent investigations, few studies have examined the effects of resveratrol supplementation associated with exercise. Resveratrol, a member of the family of polyphenols, it is well known for its cardioprotective properties. The properties of resveratrol include anti-inflammatory, anticancer, anti-aging, antioxidant and anti-degenerative. During the training of firefighters from the Military Police of São Paulo, within the discipline of Physical Training, students are regularly subjected to tests to assess their physical fitness. This test can significantly increase the physiological stress imposed on the student, increasing the risk of injury and decline in physical performance due to fatigue, muscle damage, inflammation and oxidative stress, especially after a physical fitness test (TAF). The objective of this study is to evaluate the oxidative profile in plasma and erythrocytes of a group of firefighters undergo a TAF before and after chronic supplementation with 100 mg/day of resveratrol or placebo for 3 months. The students, 60 in total (30 in resveratrol and 30 in the placebo group) will be randomly selected within the corporation and receive or resveratrol or placebo. Both the students and the researchers involved will not know who will be receiving resveratrol or placebo (double blind study). The assessments to be carried out before and after supplementation with resveratrol and before and after the TAF include: oxidative damage in biomolecules (lipids, DNA and proteins), total antioxidant activity of plasma (FRAP assay), content of reduced glutathione and oxidized glutathione , ratio GSH/GSSG, creatine kinase (CK), lactate dehydrogenase (LDH), glutamic oxaloacetic transaminase (SGOT), glutamic pyruvic transaminase (ALT), gamma-glutamyl transferase (GGT), uric acid, creatinine, total iron, mieoloperoxidase ( MPO), release of cytokines (IL-6, TNF-alpha, IL-1beta), total activity of the enzymes SOD, catalase, glutathione peroxidase and reductase. Understanding the biochemical responses in this population of firefighters during their training will contribute to understanding the changes that occur due to training and may provide important benefits to the adequacy of exercise without risks to health of this population. In addition, the characterization of the effects of chronic supplementation with resveratrol may contribute to understanding the actions of this molecule with important effects on several systems. (AU)