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Contribution of biomechanical forces to periodontal destruction in vitro and in vivo

Grant number: 11/13752-5
Support Opportunities:Regular Research Grants
Duration: November 01, 2011 - April 30, 2014
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Joni Augusto Cirelli
Grantee:Joni Augusto Cirelli
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated researchers:Carlos Rossa Junior ; James Deschner


Several studies have been conducted to understand distinctly the effects of periodontal diseases and of orthodontic movements on the periodontal tissues. But the effects of both processes presented together have not been fully elucidated. The aim of these in vitro and in vivo studies is to investigate the interactions of microbial and biomechanical signals on models that mimic the influence of mechanical orthodontic forces in periodontal disease progression and tissue destruction. The in vivo study consists of 126 rats that will be divided into four groups: 1) control (CONTR), 2) ligature-induced periodontal disease (PD), 3) orthodontic movement (OM), 4) PD followed by OM (OMPD). Periodontal disease will be induced 5 days before the orthodontic appliances placement and the sacrifice will be 3, 7, and 15 days after OM induction. Inflammatory cytokines and receptors expression will be evaluated by PCR Array and the six cytokines best expressed will be also evaluated by real time PCR for validation expression. In addition, these mediators will be evaluated by a protein analysis Western blot and ELISA. Histological sections will be obtained for descriptive and stereometry histological evaluation. Some hemimaxilas will be used for bone loss analysis by microcomputed tomography. The in vitro study will be performed to evaluate the induction of osteoblastic differentiation of pre-osteoblastic cells (MC3T3-E1) through the expression of ALP, Runx2, osteocalcin and osterix; osteoclastogenesis in macrophages (RAW 264.7) stimulated or not with LPS by TRAP staining, and the inflammatory response of periodontal ligament cells of mice (mPDL) through the expression of IL-17, IL-18, IL-33, and caspase-1. The cells will be divided into four groups: Group I - cells with no stimulation - control, Group II: cells stimulated with tension forces, Group III: cells stimulated with lipopolysaccharide, Group IV: cells stimulated with lipopolysaccharide and tension forces. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BOAS NOGUEIRA, ANDRESSA VILAS; DE MOLON, RAFAEL SCAF; NOKHBEHSAIM, MARJAN; DESCHNER, JAMES; CIRELLI, JONI AUGUSTO. Contribution of biomechanical forces to inflammation-induced bone resorption. Journal of Clinical Periodontology, v. 44, n. 1, p. 31-41, . (10/07771-4, 11/13752-5)
NOGUEIRA, ANDRESSA V. B.; NOKHBEHSAIM, MARJAN; TEKIN, SEMA; DE MOLON, RAFAEL S.; SPOLIDORIO, LUIS C.; MEMMERT, SVENJA; DAMANAKI, ANNA; JAEGER, ANDREAS; EICK, SIGRUN; DESCHNER, JAMES; et al. Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo Studies. Mediators of Inflammation, v. 2020, . (10/07771-4, 14/20715-7, 17/07137-2, 11/13752-5)
BOAS NOGUEIRA, ANDRESSA VILAS; NOKHBEHSAIM, MARJAN; EICK, SIGRUN; BOURAUEL, CHRISTOPH; JAEGER, ANDREAS; JEPSEN, SOREN; CIRELLI, JONI AUGUSTO; DESCHNER, JAMES. Regulation of visfatin by microbial and biomechanical signals in PDL cells. CLINICAL ORAL INVESTIGATIONS, v. 18, n. 1, p. 171-178, . (10/07771-4, 11/13752-5)
NOGUEIRA, ANDRESSA VILAS BOAS; NOKHBEHSAIM, MARJAN; EICK, SIGRUN; BOURAUEL, CHRISTOPH; JAEGER, ANDREAS; JEPSEN, SOREN; ROSSA, JR., CARLOS; DESCHNER, JAMES; CIRELLI, JONI AUGUSTO. Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells. Mediators of Inflammation, . (10/07771-4, 11/13752-5)

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