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Evaluation of antimicrobial activity of essential oils on oral pathogens

Grant number: 11/14757-0
Support type:Regular Research Grants
Duration: November 01, 2011 - January 31, 2014
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Pedro Luiz Rosalen
Grantee:Pedro Luiz Rosalen
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Assoc. researchers:Ana Lúcia Tasca Gois Ruiz ; Glyn Mara Figueira ; Marta Cristina Teixeira Duarte ; Severino Matias de Alencar

Abstract

The oral cavity is inhabited by a mixed microbiota which, under determined circumstances, can overcome host defenses and act as opportunists producing some clinical manifestations. There is an interest in literature for natural products as sources of new bioactive molecules for the control of oral diseases, among these products there are essential oils and their secondary products due to their antimicrobial properties. The need to generate and add new information concerning the in vitro antimicrobial activity of essential oils, stimulated the development of this work, involving twenty species of the Germplasm Bank of the "Collection of Medicinal and Aromatic Plants - CPMA" UNICAMP. In this context, this research aims to identify the in vitro antimicrobial activity of essential oils and fractions of different species of medicinal and aromatic plants against oral pathogens, organized in planktonic and in biofilms forms, as well as identifying bioactive chemical classes present in essential oils and fractions of highest activity. Moreover, influence in the morphology of biofilms produced by essential oils and fractions selected will be evaluated which may help future studies on mechanisms of action. Also the toxicological potential will be verified in cell culture. Twenty fractions and essential oils extracted from plants belonging to the Germplasm Bank of the CPMA / CPQBA / UNICAMP will be evaluated for their antimicrobial activities against oral bacteria and yeast, using the following methods: determination of minimum inhibitory concentration (MIC) and bactericidal concentration and Fungicide Minimum (CBM / FCM) assessment of biofilm in initial phase and mature on bacterial viability (time kill), the occurrence of damage or changes in the morphology of microbial cells by scanning electron microscopy (SEM) and confocal scanning laser (CSLM); check antiproliferative activity on human skin fibroblasts ATCC CRL-2522TM, Vero cells, and tumor cell lines. Bioactive chemical classes present in the oils and fractions of higher activity and yield will be identified by RP-HPLC and GC-MS. This work will determine the antimicrobial potentiality of essential oils against oral microorganisms and provide additional information to the Germplasm Bank of CPQBA / UNICAMP and to the literature, to contribute to future studies regarding the mechanism of action, toxicology and chemical isolation of compounds may be promising for the generation of drug formulations for dental application. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FREIRES, IRLAN ALMEIDA; DENNY, CARINA; BENSO, BRUNA; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. Antibacterial Activity of Essential Oils and Their Isolated Constituents against Cariogenic Bacteria: A Systematic Review. Molecules, v. 20, n. 4, p. 7329-7358, APR 2015. Web of Science Citations: 70.
FREIRES, IRLAN ALMEIDA; BUENO-SILVA, BRUNO; DE CARVALHO GALVAO, LIVIA CAMARA; TEIXEIRA DUARTE, MARTA CRISTINA; SARTORATTO, ADILSON; FIGUEIRA, GLYN MARA; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis. Evidence-based Complementary and Alternative Medicine, 2015. Web of Science Citations: 6.
FREIRES, IRLAN DE ALMEIDA; MURATA, RAMIRO MENDONCA; FURLETTI, VIVIAN FERNANDES; SARTORATTO, ADILSON; DE ALENCAR, SEVERINO MATIAS; FIGUEIRA, GLYN MARA; DE OLIVEIRA RODRIGUES, JANAINA APARECIDA; TEIXEIRA DUARTE, MARTA CRISTINA; ROSALEN, PEDRO LUIZ. Coriandrum sativum L. (Coriander) Essential Oil: Antifungal Activity and Mode of Action on Candida spp., and Molecular Targets Affected in Human Whole-Genome Expression. PLoS One, v. 9, n. 6 JUN 5 2014. Web of Science Citations: 36.
DE CARVALHO GALVAO, LIVIA CAMARA; FURLETTI, VIVIAN FERNANDES; FERNANDES BERSAN, SALETE MEYRE; DA CUNHA, MARCOS GUILHERME; TASCA GOIS RUIZ, ANA LUCIA; DE CARVALHO, JOAO ERNESTO; SARTORATTO, ADILSON; GARCIA REHDER, VERA LUCIA; FIGUEIRA, GLYN MARA; TEIXEIRA DUARTE, MARTA CRISTINA; IKEGAKI, MASARAHU; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. Antimicrobial Activity of Essential Oils against Streptococcus mutans and their Antiproliferative Effects. Evidence-based Complementary and Alternative Medicine, 2012. Web of Science Citations: 28.

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