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Development of artificial skin containing glycated dermal equivalent in the assessment of efficacy and toxicity of compound anti-glycation

Grant number: 11/14327-6
Support type:Regular Research Grants
Duration: November 01, 2011 - October 31, 2013
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:Silvya Stuchi Maria-Engler
Grantee:Silvya Stuchi Maria-Engler
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:Fernanda Branco Filippin ; Manoela Tiago dos Santos ; Mariana Teixeira Bartié ; Paula Anastácia Ferreira ; Paula Comune Pennacchi ; Raquel Brandão Haga ; Renato Ramos Massaro

Abstract

Many studies have shown variations in cellular functions, anatomical structures and molecular level in aging. The non-enzymatic glycation of proteins (AGE) seems to be a common factor to the pathophysiology of a number of age-related disorders. Dermal equivalent is an efficient model for the study of cells and caused changes in the extracellular matrix in the aging process. The appearance of the products of AGEs occurs through reactions of non-enzymatic glycation of extracellular matrix in the dermis and has been touted as one of the factors responsible for the loss of elasticity and other properties of the dermis during aging. Drug penetration is a major constraint to compounds that are expected to reach deeper layers of skin. The 3D model of artificial skin has metabolic characteristics, permeability and activity similar to the original skin, increasing its role in research on drug permeability, toxicity, irritation and differentiation of keratinocytes. A series of natural compounds or synthetic inhibitors of AGEs have been proposed recently discovered or presented and may have therapeutic use in the treatment of changes caused by the formation and accumulation of AGEs in the skin. This study aimed to evaluate the efficacy and toxicity of the compound resveratrol, Carnosine, ALT-711, benfotiamine and pyridoxamine in glycated artificial skin model. (AU)

Articles published in Pesquisa FAPESP Magazine about the research grant:
Laboratory Skin 

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CATARINO, CAROLINA MOTTER; PEDROSA, TATIANA DO NASCIMENTO; PENNACCHI, PAULA COMUNE; DE ASSIS, SILVIA ROMANO; GIMENES, FABRICIA; LOPES CONSOLARO, MARCIA EDILAINE; DE MORAES BARROS, SILVIA BERLANGA; MARIA-ENGLER, SILVYA STUCHI. Skin corrosion test: a comparison between reconstructed human epidermis and full thickness skin models. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 125, p. 51-57, APR 2018. Web of Science Citations: 1.
PEDROSA, TATIANA DO NASCIMENTO; CATARINO, CAROLINA MOTTER; PENNACCHI, PAULA COMUNE; DE ASSIS, SILVIA ROMANO; GIMENES, FABRICIA; LOPES CONSOLARO, MARCIA EDILAINE; DE MORAES BARROS, SILVIA BERLANGA; MARIA-ENGLER, SILVYA STUCHI. A new reconstructed human epidermis for in vitro skin irritation testing. TOXICOLOGY IN VITRO, v. 42, p. 31-37, AUG 2017. Web of Science Citations: 6.
PENNACCHI, PAULA COMUNE; SOARES DE ALMEIDA, MAIRA ESTANISLAU; ALVES GOMES, OCTAVIO LUIS; FAIAO-FLORES, FERNANDA; DE ARAUJO CREPALDI, MARIA CLARA; DOS SANTOS, MARINILCE FAGUNDES; DE MORAES BARROS, SILVIA BERLANGA; MARIA-ENGLER, SILVYA STUCHI. Glycated Reconstructed Human Skin as a Platform to Study the Pathogenesis of Skin Aging. TISSUE ENGINEERING PART A, v. 21, n. 17-18, p. 2417-2425, SEP 1 2015. Web of Science Citations: 16.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.