Phylogenetic analysis and study of sex and B chromosomes of Physalaemus (Anura, Leiuperidae): a contribution to the analysis of the karyological evolution in the genus

• Grant number: 11/09239-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2011
• End date: October 31, 2013
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Luciana Bolsoni Lourenço
• Grantee: Luciana Bolsoni Lourenço
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• City of the host institution: Campinas
• Associated researchers: João Reinaldo da Cruz de Campos

Abstract

The genus Physalaemus is very interesting for chromosomal evolutionary studies. Several species of this genus were already karyotyped and, among all of them, only Physalaemus ephippifer showed heteromorphic sex chromosomes, suggesting Physalaemus as a valuable genus for studies of processes involved in the sex chromosomes differentiation. Despite that, a proper comparative analysis of the available karyotypes is not possible, since interspecific chromosomal homeologies are not easily recognized. Another interesting cytogenetic finding concerning this genus is the B chromosome of P. feioi. Aiming to contribute to the chromosomal studies of this genus, one of the goals this proposal is the phylogenetic analysis of Physalaemus species. This proposal also intends a better characterization of the B chromosome of P. feioi and the chromosomes of P. ephippifer, aiming the description of new chromosomal markers for this latter, with special interest in those concerning its sex chromosomes. The phylogenetic relationships will be inferred from the nucleotide sequences of the mitochondrial genes 12S, 16S and tRNA-Val, by parsimony and Bayesian methods. For studying the molecular nature of the B chromosome of P. feioi, microdissection and chromosome painting will be made. For P. ephippifer studies, new populations will be karyotyped, repetitive DNA sequences will be isolated and chromosome painting of the sex chromosomes will be performed. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEAL, FERNANDO; ZORNOSA-TORRES, CAMILA; AUGUSTO-ALVES, GUILHERME; DENA, SIMONE; PEZZUTI, TIAGO LEITE; LEITE, FELIPE; LOURENCO, LUCIANA BOLSONI; GARCIA, PAULO; TOLEDO, LUIS FELIPE. Head in the clouds: a new dwarf frog species of the Physalaemus signifer clade (Leptodactylidae, Leiuperinae) from the top of the Brazilian Atlantic Forest. EUROPEAN JOURNAL OF TAXONOMY, v. 764, p. 119-151, AUG 19 2021. (16/25358-3, 19/03170-0, 11/09239-0, 19/18335-5)

VITTORAZZI, STENIO EDER; LOURENCO, LUCIANA BOLSONI; RECCO-PIMENTEL, SHIRLEI MARIA. Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs. BMC GENETICS, v. 15, OCT 15 2014. (11/09239-0, 10/11300-7)

LOURENCO, LUCIANA B.; TARGUETA, CINTIA P.; BALDO, DIEGO; NASCIMENTO, JULIANA; GARCIA, PAULO C. A.; ANDRADE, GILDA V.; HADDAD, CELIO F. B.; RECCO-PIMENTEL, SHIRLEI M.. Phylogeny of frogs from the genus Physalaemus (Anura, Leptodactylidae) inferred from mitochondrial and nuclear gene sequences. Molecular Phylogenetics and Evolution, v. 92, p. 204-216, NOV 2015. (13/50741-7, 08/50928-1, 11/09239-0)

NASCIMENTO, JULIANA; LIMA, JUCIVALDO D.; SUAREZ, PABLO; BALDO, DIEGO; ANDRADE, V, GILDA; PIERSON, TODD W.; FITZPATRICK, BENJAMIN M.; HADDAD, CELLO F. B.; RECCO-PIMENTEL, SHIRLEI M.; LOURENCO, LUCIANA BOLSONI. Extensive Cryptic Diversity Within the Physalaemus cuvieri-Physalaemus ephippifer Species Complex (Amphibia, Anura) Revealed by Cytogenetic, Mitochondrial, and Genomic Markers. FRONTIERS IN GENETICS, v. 10, AUG 14 2019. (14/50342-8, 13/50741-7, 16/06030-7, 11/09239-0)

GATTO, KALEB PRETTO; SOUZA, LUCAS H. B.; NASCIMENTO, JULIANA; SUAREZ, PABLO; LOURENCO, LUCIANA BOLSONI. Comparative mapping of a new repetitive DNA sequence and chromosome region-specific probes unveiling rearrangements in an Amazonian frog complex. GENOME, v. 64, n. 9, p. 857-868, SEP 2021. (17/15842-8, 11/09239-0)

LEAL, FERNANDO; LEITE, FELIPE S. F.; DA COSTA, WILLIAM P.; NASCIMENTO, LUCIANA B.; LOURENCO, LUCIANA B.; GARCIA, PAULO C. A.. Amphibians from Serra do Cipo, Minas Gerais, Brasil. VI: A New Species of the Physalemus deimaticus Group (Anura, Leptodactylidae). Zootaxa, v. 4766, n. 2, p. 306-330, APR 20 2020. (11/09239-0)