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The effect of consumption of milk protein serums on HSP biomarkers and biochemical parameters in rats

Grant number: 11/13035-1
Support type:Regular Research Grants
Duration: December 01, 2011 - November 30, 2013
Field of knowledge:Health Sciences - Nutrition
Principal Investigator:Jaime Amaya Farfan
Grantee:Jaime Amaya Farfan
Home Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Assoc. researchers:Carolina Soares de Moura

Abstract

Background. Glutamine is the most abundant plasma amino acid, which under certain circumstances could have its concentration reduced resulting in unfavorable alterations in homeostasis, such as the impairment of production of the protecting heat shock proteins (HSPs) during the practice of physical exercise. The milk whey proteins are rich in glutamine and branched-chain amino acids (BCAAs), all of which are known to be important in maintaining the plasma and muscle levels of glutamine involving the enzyme glutamine synthetase. It is possible therefore that the whey proteins can indirectly offer significant protection to the cellular system when consumed as food because of their ability to maintain high plasma levels of both glutamine and BCAAs. Objective. To assess the influence of consuming the whey proteins (both intact [WP] hydrolyzed [HWP]) on: (a) the plasma levels of glutamine and BCAAs, (b) the concentration of HSPs and (c) the homeostasis of rats submitted to physical training the treadmill. Methods. Forty-eight male Wistar rats will be divided into two primary groups, namely sedentary and trained, each in turn subdivided into 3 dietary groups (WP, HWP and Casein as control) using the basal AIN 93-G standard rat formulation. The following blood parameters will be determined: uric acid, creatinine, CK, LDH, glucose, corticosterone, AST, ALT, total proteins, urea and free amino acids. In other tissues, gastrocnemial free amino acids, carbonyl proteins, glycogen, HSP70, 60, 90, GFAT, OGT, HSF1 and glutamine synthetase; soleal HSP70, 60 and 90, and glutamine synthetase; myocardial glycogen, HSP70, 60, 90, GFAT, OGT and HSF1. In the lung and spleen, HSP70 and glutamine synthetase will also be determined, in addition to pre- and post-exercise body temperature. The data will receive statistical treatment by ANOVA adopting standard criteria of significance. (AU)

Articles published in Agência FAPESP Newsletter about the research grant
Hydrolysate milk protein serum can help to combat hyperglycemia 

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARINELI, RAFAELA DA SILVA; MOURA, CAROLINA SOARES; MORAES, ERICA AGUIAR; LENQUISTE, SABRINA ALVES; BARBOZA LOLLO, PABLO CHRISTIANO; MORATO, PRISCILA NEDER; AMAYA-FARFAN, JAIME; MAROSTICA, JR., MARIO ROBERTO. Chia (Salvia hispanica L.) enhances HSP, PGC-1 alpha expressions and improves glucose tolerance in diet-induced obese rats. NUTRITION, v. 31, n. 5, p. 740-748, MAY 2015. Web of Science Citations: 24.
MOURA, CAROLINA SOARES; BARBOZA LOLLO, PABLO CHRISTIANO; MORATO, PRISCILA NEDER; NISISHIMA, LUCIANA HISAYAMA; CARNEIRO, EVERARDO MAGALHAES; AMAYA-FARFAN, JAIME. Whey Protein Hydrolysate Enhances HSP90 but Does Not Alter HSP60 and HSP25 in Skeletal Muscle of Rats. PLoS One, v. 9, n. 1 JAN 20 2014. Web of Science Citations: 12.
LOLLO, PABLO C. B.; MOURA, CAROLINA S.; MORATO, PRISCILA N.; AMAYA-FARFAN, JAIME. Differential Response of Heat Shock Proteins to Uphill and Downhill Exercise in Heart, Skeletal Muscle, Lung and Kidney Tissues. JOURNAL OF SPORTS SCIENCE AND MEDICINE, v. 12, n. 3, p. 461-466, SEP 2013. Web of Science Citations: 20.
DE MOURA, CAROLINA SOARES; BARBOZA LOLLO, PABLO CHRISTIANO; MORATO, PRISCILA NEDER; CARNEIRO, EVERARDO MAGALHAES; AMAYA-FARFAN, JAIME. Whey protein hydrolysate enhances the exercise-induced heat shock protein (HSP70) response in rats. Food Chemistry, v. 136, n. 3-4, p. 1350-1357, FEB 1 2013. Web of Science Citations: 20.

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