Purification, properties and application of xylanolytic enzymes produced by Penicillium janczewskii

• Grant number: 10/16582-0
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2011 - November 30, 2013
• Field of knowledge: Agronomical Sciences - Food Science and Technology
• Principal Investigator: Eleonora Cano Carmona
• Grantee: Eleonora Cano Carmona
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated researchers: César Rafael Fanchini Terrasan

Abstract

Fungi have been exploited for the enzyme production due to the facility to secrete large amounts of proteins in the extracellular medium. Many enzymes are used in different industrial processes which, considering the annual growth of the enzyme market, has stimulated studies on the search of new microbial strains and on the development of new production processes. Xylanases and b-xylosidases are the main enzymes involved in the degradation of xylan, the second main component of the plant cell wall, and they can be used in many processes in the food and beverages, feed, textile and pulp and paper industries. A Penicillium janczewskii strain isolated from Brazilian soil was studied in relation to the optimal conditions for xylanase and b-xylosidase production. The objective of this work is to purify, characterize the main biochemical properties of these enzymes and also apply them in the biobleaching process of cellulose pulp. This way, this project can also be inserted in a current context aiming to reduce the environmental pollution. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FANCHINI TERRASAN, CESAR RAFAEL; MANUEL GUISAN, JOSE; CARMONA, ELEONORA CANO. Xylanase and beta-xylosidase from Penicillium janczewskii: Purification, characterization and hydrolysis of substrates. ELECTRONIC JOURNAL OF BIOTECHNOLOGY, v. 23, p. 54-62, SEP 2016. (07/08640-8, 10/16582-0)

FANCHINI TERRASAN, CESAR RAFAEL; ROMERO-FERNANDEZ, MARIA; ORREGO, ALEJANDRO H.; MARTINS OLIVEIRA, SANDRO; COSTA PESSELA, BENEVIDES; CANO CARMONA, ELEONORA; MANUEL GUISAN, JOSE. Immobilization and Stabilization of Beta-Xylosidases from Penicillium janczewskii. Applied Biochemistry and Biotechnology, v. 182, n. 1, p. 349-366, MAY 2017. (10/16582-0)

TERRONE, CAROL CABRAL; DE FREITAS, CAROLINE; FANCHINI TERRASAN, CESAR RAFAEL; DE ALMEIDA, ALEX FERNANDO; CARMONA, ELEONORA CANO. Agroindustrial biomass for xylanase production by Penicillium chrysogenum: Purification, biochemical properties and hydrolysis of hemicelluloses. ELECTRONIC JOURNAL OF BIOTECHNOLOGY, v. 33, p. 39-45, MAY 15 2018. (10/16582-0)

CÉSAR RAFAEL FANCHINI TERRASAN; JOSÉ MANUEL GUISAN; ELEONORA CANO CARMONA. Xylanase and β-xylosidase from Penicillium janczewskii: Purification, characterization and hydrolysis of substrates. ELECTRONIC JOURNAL OF BIOTECHNOLOGY, v. 19, n. 5, p. 54-62, 2016-09-00. (10/16582-0, 07/08640-8)