| Grant number: | 11/13869-0 |
| Support Opportunities: | Regular Research Grants |
| Start date: | January 01, 2012 |
| End date: | June 30, 2014 |
| Field of knowledge: | Agronomical Sciences - Agronomy - Plant Health |
| Principal Investigator: | Valdenice Moreira Novelli |
| Grantee: | Valdenice Moreira Novelli |
| Host Institution: | Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Campinas , SP, Brazil |
| City of the host institution: | Campinas |
| Associated researchers: | Carlos Alberto Labate ; Elliot Watanabe Kitajima ; Juliana de Freitas Astúa ; Maria Andreia Nunes ; Marinês Bastianel |
Abstract
The Brevipalpus mites have been the focus of attention for its ability to transmit virus to several different plants. Three major economically important crops - citrus, coffee and passion fruit - are targets of fitovirus transmitted by this mite. Citrus leprosis is a disease of major impact, given the high cost with the use of acaricides in orchards to control mites. Strategies to reduce the cost and environmental impact are extremely desirable. In recent years there has been a significant advance in knowledge leprosis virus, cytoplasmic type (CiLV-C), including diagnosis by molecular tests and the synthesis of polyclonal antibodies for immunological detection and, more recently, data from microscopy and real-time PCR (qPCR) suggest that the interaction virus-vector is circulative. However, although there is much information about the CiLV-C, little is known about the biological aspects of the mite and the influence of CiLV-C, or about parameters involved in its transmission. In this work the objectives are to study embryology and development of B. phoenicis; assess whether the virus interferes in the reproductive and longevity of the mite, considering the presence of symbionts, establish biological phase in which the vector is more efficient for the transmission of the virus; estimate the concentration of the symbiont in the life stages of the mite, and to obtain protein profile in viruliferous and aviruliferous mites. It is hoped more information about the interactions that occur in leprosis pathosystem, which may be useful to propose new alternatives for the management and control of disease. (AU)
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