Influence of diet supplementation of rosemary (Rosmarinus oficinalis L.) on cardiac remodeling in rats subjected to acute myocardial infarction

• Grant number: 11/15059-5
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2011 - November 30, 2013
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Sergio Alberto Rupp de Paiva
• Grantee: Sergio Alberto Rupp de Paiva
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Katashi Okoshi ; Leonardo Antonio Mamede Zornoff ; Marcos Ferreira Minicucci ; Paula Schmidt Azevedo Gaiolla

Abstract

Cardiac remodeling has a key role in ventricular dysfunction after myocardial infarction. Some cellular mechanisms such as oxidative stress, inflammation and fibrosis modulate this process. Thus, treatment or repair of post-infarction changes has great clinical relevance. There is great interest in natural products with antioxidant properties, especially rosemary. Objective: To analyze the influence of the consumption of dietary rosemary extract on cardiac remodeling after acute myocardial infarction (AMI) in rats. Methods: Wistar male rats with 200-250g will be allocated in six groups: 1) Sham group fed cereal-based chow and not subjected to AMI (SA0, n = 30), 2) Sham group fed with chow supplemented with 0.02% rosemary extract (diet 1) and not subjected to AMI (SA1, n = 30), 3) Sham group fed cereal-based chow supplemented with 0.2% extract rosemary (diet 2) and not subjected to AMI (SA2, n = 20), 4) AMI + diet based on cereals (IA0, n = 30), 5) AMI + diet 1 (IA1, n = 30; 6 ) AMI + diet 2 (IA2, n = 30). After three months, echocardiography study and euthanasia of animals for collection of biological material will be done. Cardiac tissue will be evaluated: 1) morphometric study, 2) analysis of oxidative stress by SOD, catalase and GPx and peroxidation, superoxide production and NADPH oxidase activity, 3) analysis of Nrf-2, HO-1 and GPx, 4) apoptosis study, 5) evaluation of inflammatory cytokines and 6) activity of metalloproteinases. Comparisons will be made by two-way ANOVA. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MURINO RAFACHO, BRUNA PAOLA; DOS SANTOS, PRISCILA PORTUGAL; GONCALVES, ANDREA DE FREITAS; HENRIQUE FERNANDES, ANA ANGELICA; OKOSHI, KATASHI; CHIUSO-MINICUCCI, FERNANDA; AZEVEDO, PAULA S.; MAMEDE ZORNOFF, LEONARDO ANTONIO; MINICUCCI, MARCOS FERREIRA; WANG, XIANG-DONG; et al. Rosemary supplementation (Rosmarinus oficinallis L.) attenuates cardiac remodeling after myocardial infarction in rats. PLoS One, v. 12, n. 5, MAY 11 2017. (11/15059-5, 11/08956-0)