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Jasmonates production in bioreactors

Grant number: 11/18978-1
Support type:Regular Research Grants
Duration: February 01, 2012 - July 31, 2014
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Miriam Verginia Lourenço
Grantee:Miriam Verginia Lourenço
Home Institution: Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil
Assoc. researchers:Murilo Daniel de Mello Innocentini ; Suzelei de Castro França

Abstract

Jasmonates (JAs) are a group of plant hormones produced in most plants which help regulate plant growth and development and play a role in plant defense mechanism. More recently their efficacy against some types of cancer has been investigated. Actually, jasmonatos are extracted from plants but their low productivity makes them expensive. However, biotechnological processes involving microorganisms can improve jasmonates production e.g. batch-fermentations in a bioreactor using the filamentous fungus Botryosphaeria rhodina. The use of microorganisms for the development of innovative pharmacological products is of great interest but further biotechnological approaches must be carried out to make viable large scale production. The right choice of high producer strains as well as the determination of the bioprocess conditions for obtaining the product of interest is essential for establishing the production process. In view of previous results when B. rhodina jasmonate-producer strains have been identified, our goal is in the present work is to establish ideal conditions for jasmonates production by B. rhodina Kifn 3.1 strain in 2-5 liters bioreactors, under controlled conditions. Initially, batch-fermentation assays will be carried out using 250 mL Erlenmeyers containing culture medium added with peptone, tryptone, glycine, the precursor linolenic acid and supplemented with different concentrations and combinations of inorganic salts to verify the effects of those compounds on jasmonate production. Moreover, fermentations will be carried out in 2-5 liters commercial reactor, operating in batch, continuous and fed-batch regime. The system will be maintained in the dark under 30ºC. Temperature, pH, substrate feeding and aeration conditions will be monitored and controlled using the fermenter control panel. Jasmonates production will be evaluated by extraction with organic solvents and further quantification using HPLC-DAD. Different extractors such as ion exchange resins and nanofiltration membranes will be tested using new extraction methods in order to minimize organic solvents use. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LOURENCO, MIRIAM VERGINIA; SOARES, WELLINGTON; BERTONI, BIANCA WALERIA; OLIVEIRA, ANA PAULA; VAZ PEREIRA, SARAZETE IZIDIA; SOARES PEREIRA, ANA MARIA; FRANCA, SUZELEI CASTRO. Eliciting effect of jasmonates from Botryosphaeria rhodina enhances coumarin production in Mikania laevigata plants. PLANT CELL TISSUE AND ORGAN CULTURE, v. 125, n. 3, p. 595-598, JUN 2016. Web of Science Citations: 2.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.