| Grant number: | 11/18978-1 |
| Support Opportunities: | Regular Research Grants |
| Start date: | February 01, 2012 |
| End date: | July 31, 2014 |
| Field of knowledge: | Biological Sciences - Microbiology - Applied Microbiology |
| Principal Investigator: | Miriam Verginia Lourenço |
| Grantee: | Miriam Verginia Lourenço |
| Host Institution: | Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil |
| Associated researchers: | Murilo Daniel de Mello Innocentini ; Suzelei de Castro França |
Abstract
Jasmonates (JAs) are a group of plant hormones produced in most plants which help regulate plant growth and development and play a role in plant defense mechanism. More recently their efficacy against some types of cancer has been investigated. Actually, jasmonatos are extracted from plants but their low productivity makes them expensive. However, biotechnological processes involving microorganisms can improve jasmonates production e.g. batch-fermentations in a bioreactor using the filamentous fungus Botryosphaeria rhodina. The use of microorganisms for the development of innovative pharmacological products is of great interest but further biotechnological approaches must be carried out to make viable large scale production. The right choice of high producer strains as well as the determination of the bioprocess conditions for obtaining the product of interest is essential for establishing the production process. In view of previous results when B. rhodina jasmonate-producer strains have been identified, our goal is in the present work is to establish ideal conditions for jasmonates production by B. rhodina Kifn 3.1 strain in 2-5 liters bioreactors, under controlled conditions. Initially, batch-fermentation assays will be carried out using 250 mL Erlenmeyers containing culture medium added with peptone, tryptone, glycine, the precursor linolenic acid and supplemented with different concentrations and combinations of inorganic salts to verify the effects of those compounds on jasmonate production. Moreover, fermentations will be carried out in 2-5 liters commercial reactor, operating in batch, continuous and fed-batch regime. The system will be maintained in the dark under 30ºC. Temperature, pH, substrate feeding and aeration conditions will be monitored and controlled using the fermenter control panel. Jasmonates production will be evaluated by extraction with organic solvents and further quantification using HPLC-DAD. Different extractors such as ion exchange resins and nanofiltration membranes will be tested using new extraction methods in order to minimize organic solvents use. (AU)
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