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New parameters for tooth bleaching: an evaluation of efficacy, citotoxicity and molecular effects

Abstract

The in-office bleaching is a commonly used technique because the clinical results are quickly obtained. However, in the way that this technique has been applied nowadays, the risks of irreversible deleterious effects on pulp tissue are high. Thereafter, the aim of this study is evaluated the effectiveness, cytotoxicity and molecular effects of new in-office bleaching protocols. Enamel/dentin discs, obtained from bovine incisors, will be selected by reflectance spectrofotometer, to standardize the enamel colour. Then, the discs will be submitted to 3 bleach sections, composed by 1 or 3 applications of a bleaching gel with 15 or 35% of hydrogen peroxide (HP) for 5 or 15 minutes, or with a 37% carbamide peroxide (CP) bleaching gel for 10 or 20 minutes. The colour will be analysed after each section. In order to evaluate the trans-enamel and trans-dentinal cytotoxicity, enamel/dentin discs will be adapted to artificial pulp chambers. Odontoblast like-cells MDPC-23 and primary culture of human pulp cells (HPC) will be seeded on the dentin side of the discs and the bleaching protocols will be done on enamel side. The cell metabolism, phosphatase alcalin activity and cell morphology will be analysed, and the total of HP capable to make the trans-enamel and trans-dentinal diffusion will be quantified. The genes of inflammatory mediators and differentiation markers for odontoblasts will be analysed for PCR real time, and the calcified nodule formation will be measured on HPC. The data obtained will be submitted to specific statistical tests. (AU)

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Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOARES, DIANA GABRIELA; BASSO, FERNANDA GONCALVES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Immediate and late analysis of dental pulp stem cells viability after indirect exposition to alternative in-office bleaching strategies. CLINICAL ORAL INVESTIGATIONS, v. 19, n. 5, p. 1013-1020, JUN 2015. Web of Science Citations: 15.
DE OLIVEIRA DUQUE, CARLA CAROLINE; SOARES, DIANA GABRIELA; BASSO, FERNANDA GONCALVES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Bleaching effectiveness, hydrogen peroxide diffusion, and cytotoxicity of a chemically activated bleaching gel. CLINICAL ORAL INVESTIGATIONS, v. 18, n. 6, p. 1631-1637, JUL 2014. Web of Science Citations: 12.
SOARES, DIANA G.; BASSO, FERNANDA G.; PONTES, ELAINE C. V.; GARCIA, LUCAS DA F. R.; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS A. Effective tooth-bleaching protocols capable of reducing H2O2 diffusion through enamel and dentine. Journal of Dentistry, v. 42, n. 3, p. 351-358, MAR 2014. Web of Science Citations: 26.
SOARES, DIANA GABRIELA; BASSO, FERNANDA GONCALVES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Concentrations of and application protocols for hydrogen peroxide bleaching gels: Effects on pulp cell viability and whitening efficacy. Journal of Dentistry, v. 42, n. 2, p. 185-198, FEB 2014. Web of Science Citations: 49.
SOARES, DIANA GABRIELA; PASTANA, JULIA VIEIRA; DE OLIVEIRA DUQUE, CARLA CAROLINE; DIAS RIBEIRO, ANA PAULA; BASSO, FERNANDA GONCALVES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Influence of Adhesive Restorations on Diffusion of H2O2 Released from a Bleaching Agent and Its Toxic Effects on Pulp Cells. Journal of Adhesive Dentistry, v. 16, n. 2, p. 123-128, 2014. Web of Science Citations: 5.
VARGAS, FERNANDA DA SILVEIRA; SOARES, DIANA GABRIELA; DIAS RIBEIRO, ANA PAULA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2O2 Induced Toxicity on Dental Pulp Cells. BIOMED RESEARCH INTERNATIONAL, 2014. Web of Science Citations: 9.
SOARES, DIANA GABRIELA; DIAS RIBEIRO, ANA PAULA; VARGAS, FERNANDA DA SILVEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Efficacy and cytotoxicity of a bleaching gel after short application times on dental enamel. CLINICAL ORAL INVESTIGATIONS, v. 17, n. 8, p. 1901-1909, NOV 2013. Web of Science Citations: 36.
SOARES, DIANA GABRIELA; VOLTOLINE PONTES, ELAINE CRISTINA; DIAS RIBEIRO, ANA PAULA; BASSO, FERNANDA GONCALVES; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Low toxic effects of a whitening strip to cultured pulp cells. AMERICAN JOURNAL OF DENTISTRY, v. 26, n. 5, p. 283-285, OCT 2013. Web of Science Citations: 5.
DIANA GABRIELA SOARES; ANA PAULA DIAS RIBEIRO; ADRIANO FONSECA LIMA; NANCY TOMOKO SACONO; JOSIMERI HEBLING; CARLOS ALBERTO DE SOUZA COSTA. Effect of Fluoride-Treated Enamel on Indirect Cytotoxicity of a 16% Carbamide Peroxide Bleaching Gel to Pulp Cells. Brazilian Dental Journal, v. 24, n. 2, p. 121-127, Abr. 2013.

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