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Leukogram and serum profile, specially acute phase proteins and immunoglobulins, in experimental mastitis induced by Staphylococcus aureus and Streptococcus agalactiae

Abstract

The aim of this project is to determine the leukon count and serum biochemical profile, especially of acute phase proteins (APPs) and immunoglobulins, of goats undergoing experimental intramammary infection caused by Staphylococcus aureus and Streptococcus agalactiae. We wish to verify the importance of APPs as biomarkers of mastitis and identify the main APPs involved in the acute phase response in this species. For such, 20 mammary quarters from 10 adult crossbreed goats (Boer x Saanen x Anglo-nubian), will be selected after a complete physical exam and examination of the mammary glands and also after obtaining negative results for the strip cup test, California Mastitis Test e microbiologic analysis of milk. The ten females will be randomly allotted into two groups: G1 - experimental intramammary infection by Staphylococcus aureus and GII - experimental intramammary infection by Streptococcus agalactiae. Each group will contain five goats and each female will have one teat infected while the contralateral one will act as the control teat. Once the experiment has started, the animals will be submitted to daily clinical exam and examination of the mammary gland as well as daily blood and milk sampling during seven consecutive days which will be identified as M0, M1, M2, M3, M4, M5 and M6. The first blood and milk collection (M0) will take place before the induction of infection. By the end of this period systemic antibiotical therapy as well as local treatment will be instituted, as recommended for infections caused by the agents used. (AU)