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Plasma proteome and homocysteine in pediatric systemic lupus erythematosus

Grant number: 11/16141-7
Support type:Regular Research Grants
Duration: March 01, 2012 - February 28, 2014
Field of knowledge:Health Sciences - Nutrition
Principal Investigator:Jacqueline Pontes Monteiro
Grantee:Jacqueline Pontes Monteiro
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Assoc. researchers: Roberta Garcia Salomão

Abstract

To date, no risk factor or group of risk factors has been uniformly helpful in predicting which patients with Systemic Lupus Erythematosus (SLE) will have atherosclerotic cardiovascular events. The primary aim of this study was to correlate groups of LES patients that present different results for a subset of risk factors, with proteome and food intake profiles. Secondary objectives: (a) to describe homocysteine, vitamin B12, folate, lipoproteins, C-reactive protein, MCP-1, Interferon alpha, IL-6, TNF-a, leptin, adiponectin and ghrelin concentrations,in LES patients and in their healthy controls. (b)To describe proteomic profile in female LES patients comparing to their healthy controls. (c) To determine the MTHFR gene mutations frequencies of the CT genotype, AC genotype and GA genotype and correlate with Homocysteine level in pediatric patients with SLE and in healthy control group. (d) To correlate polimorphism in MTHFR with homocysteine levels and with food intake for energy, macronutrients, folic acid, vitamin B12 and vitamin B6. Methods: Fasting serum samples will be collected from 50 pediatric patients with SLE, age between 10 to 18 years old who attend the Pediatric Lupus Clinic at Medicine Faculty Hospital, Ribeirão Preto, Brazil. A hundred healthy children paired for gender and age will be the control group. Values < 12 mmol/L will be adopted as the normal range for plasma homocysteine levels. Genomic DNA of leucocytes will be extracted. The presence of C677T, A1298C and G1793A mutation in gene enconding the MTHFR enzyme will be determined by PCR-restriction fragment length polymorphism (RFLP). Vitamin B12, folate and IL-6 will be determined using a immunoanalysis assay. ELISA Kit will be used to determine leptin, adiponectin, grelin, TNF-±, MCP-1. For proteome analysis the i-TRAQ, Applied Biosystems will be used. All children will be submited to nutritional assessment. ANCOVA will be used. The level of significance will set at p <0.05 for all tests. Qui-square or Fisher, k-cluster and hierarchical cluster will be applied. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SALOMAO, ROBERTA GARCIA; DE CARVALHO, LUCIANA MARTINS; IZUMI, CLARICE; CZERNISZ, ERIKA SILVA; ROSA, JOSE CESAR; RAUBER ANTONINI, SONIR ROBERTO; BUENO, ANA CAROLINA; RIBEIRO DO VALE ALMADA, MARIA OLIMPIA; COELHO-LANDELL, CAROLINA DE ALMEIDA; JORDAO, ALCEU AFONSO; LEME FERRIANI, VIRGINIA PAES; MONTEIRO, JACQUELINE PONTES. Homocysteine, folate, hs-C-reactive protein, tumor necrosis factor alpha and inflammatory proteins: are these biomarkers related to nutritional status and cardiovascular risk in childhood-onset systemic lupus erythematosus?. PEDIATRIC RHEUMATOLOGY, v. 16, JAN 9 2018. Web of Science Citations: 1.

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