| Grant number: | 11/21531-9 |
| Support type: | Regular Research Grants |
| Duration: | March 01, 2012 - February 28, 2014 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery |
| Principal Investigator: | Fabiano Antonio Cadioli |
| Grantee: | Fabiano Antonio Cadioli |
| Home Institution: | Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil |
| Assoc. researchers: | Luiz Carlos Marques ; Rosangela Zacarias Machado |
Abstract
The goal of this project is determines the electrophoretic profile of the serum proteins and humoral immune response of Girolanda cattle naturally infected with Trypanosoma vivax, using 500 serum samples, already collected and stored under refrigeration (-80ºC), from a naturally infected herd and 50 serum samples from healthy Girolanda cattle, from non-endemic area for this hemoparasite. For serum protein identification and quantification, electrophoretic fractionation will be carried out in polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE), and Indirect Fluorescent Antibody Test (IFAT) and Enzime-linked Immunosorbent assay (ELISA test) will be performed by for anti-T. vivax antibody's determination. (AU)