The public health laboratories have as principal activity promoting activities related on epidemiological surveillance of the population. Adolfo Lutz Institute is formed by a group of laboratories whose mission is to produce knowledge to public health; development and diffusion of scientific research; standardization of different methodologies; diagnostic and analytical methods; and employee improvement. "Centro de Parasitologia e Micologia" develops exactly this work, but specifically for studies correlated with parasites and fungi. The "Laboratorio de Biologia Molecular de Parasitas e Fungos" has made and fulfilled the same tasks, but it offers the tools of molecular and immunological methods. Opportunistic infections have been the target of our studies, mainly correlated to Aids and official programs such as the "Network of leishmaniasis in the State of Sao Paulo". The necessity a rapid and accurate diagnosis has led us to establish molecular methods for different infections caused by parasites. Studies related to the immune response of infected hosts also have been addressed. The present project aims to investigate some aspects of four important diseases in public health. In the first part will be develop a study of the co-infection Aids and the two most prevalent opportunistic neurological diseases in Brazil. Cellular and humoral immune response elicited by cerebral toxoplasmosis and cryptococcal meningitis in Aids patients before and after treatment will be studied. One part of the investigation will be conducted in experimental model and then correlated with the human infection. The profiles of antibody classes and cytokines elicited by Toxoplasma gondii infection in immunosuppressed mice will be analyzed. These studies will be conducted in order to establish the immune response involved before and after cerebral toxoplasmosis treatment. In parallel, the human investigations will be performed in Aids patients with cerebral toxoplasmosis. The results will be compared with immunocompetent patients with asymptomatic or active infection (the ocular form). In another step of this project, the same investigations will be performed in Aids patients with cryptococcal meningitis. In parallel, the molecular diagnosis of cryptococcosis in samples of cerebrospinal fluid will be introduced. This methodology will be able to genotyping of the infecting strain as well as identification of the agent and the evaluation of the reduction of fungal burden during antifungal treatment. The second part of this study will be dedicated to determine the genotypic patterns of the two Leishmania species prevalent in the State of São Paulo, L. (V.) braziliensis and L. (L.) infantum chagasi. In parallel, the possibility of controlling the treatment of patients with cutaneous leishmaniasis with the polymerase chain reaction in blood and serological tests will be investigated. (AU)
Articles published in Agência FAPESP Newsletter about the research grant:
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COSTA FERREIRA, ANA IARA;
BRANDAO DE MATTOS, CINARA CASSIA;
FREDERICO, FABIO BATISTA;
BERNARDO, CASSIA RUBIA;
DE ALMEIDA JUNIOR, GILDASIO CASTELO;
SIQUEIRA, RUBENS CAMARGO;
MEIRA-STREJEVITCH, CRISTINA SILVA;
PEREIRA-CHIOCCOLA, VERA LUCIA;
DE MATTOS, LUIZ CARLOS.
Duffy blood group system and ocular toxoplasmosis.
INFECTION GENETICS AND EVOLUTION,
Web of Science Citations: 0.
FERREIRA, A. I. C.;
BRANDAO DE MATTOS, C. C.;
FREDERICO, F. B.;
MEIRA, C. S.;
ALMEIDA, JR., G. C.;
BERNARDO, C. R.;
PEREIRA-CHIOCCOLA, V. L.;
DE MATTOS, L. C.
Risk factors for ocular toxoplasmosis in Brazil.
EPIDEMIOLOGY AND INFECTION,
Web of Science Citations: 22.