Microencapsulation and nanoencapsulation of natural compounds by drying technologies

• Grant number: 11/10333-1
• Support Opportunities: Research Grants - Young Investigators Grants
• Duration: April 01, 2012 - March 31, 2017
• Field of knowledge: Engineering - Chemical Engineering - Industrial Operations and Equipment for Chemical Engineering
• Principal Investigator: Cláudia Regina Fernandes de Souza
• Grantee: Cláudia Regina Fernandes de Souza
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 14/01105-3 - Solid lipid nanoparticles containing bioactive compounds of Petiveria alliacea (Phytolaccaceae), BP.IC; 12/13648-6 - MICROENCAPSULATION OF BIOACTIVE COMPOUNDS OF CAMELIA SINENSIS IN SOLID LIPIDIC SYSTEMS, BP.MS; 12/09890-6 - Encapsulation of bioactive compounds from Syzygium aromaticum in solid lipidic carriers, BP.DR; 12/03427-2 - Microencapsulation and nanoencapsulation of natural compounds by drying technologies, BP.JP

Abstract

Many natural products of commercial importance, particularly in food and pharmaceutical industries, are complex mixtures of various substances. Examples herbal substances (phytochemicals) with claimed health benefits include the phenolic compounds (ex. curcumin, resveratrol, epigallocatechin gallate, and so on) and carotenoids (ex. lycopene, lutein, zeaxanthin, b-carotene, etc.). However, the biological effect can be drastically diminished or lost after oral administration, due to low solubility of the compound in the tract gastrointestinal (low absorption), and the effect of presystemic metabolism. Other products are volatile (ex essential oils), thermo-sensitive and susceptible to oxidation and may undergo irreversible changes in their physical and chemical properties when exposed to unprotected contact with other materials or external agents. Micro/Nanoencapsulation technologies have a huge potential application in the natural products area permitting, for example, the transformation of liquid and volatile products into solids, protection and modulation of the delivery of active compounds, enabling a better absorption of hydrophilic and lipophilic constituents. In this way, several technical limitations that currently impossibility the development of innovative products containing natural ingredients, with great potential for use in various industrial sectors such as the food and pharmaceutical can be overcome. For example, the low solubility of a natural product could adversely affect its applicability and performance, leading to difficulty in the formulating and probably low bioavailability, often requiring the use of excessive amounts.Among the strategies that can be employed for the production of micro and nanostructured systems containing natural products include the micro/nanoencapsulation by physical processes (spray drying, air suspension, freeze drying, spray chilling, micronization, high pressure homogenization, and microfluidization) or by physicochemical ones (simple and complex coacervation, solvent evaporation, etc). Different wall materials may be employed, such as proteins and polysaccharides, derivatives of acrylic and methacrylic acids, polylactic/polyglycolic acid, lipids, sugars, organic acids, and hydrogenated vegetable oil. The inclusion with cyclodextrins and molecular encapsulation into liposomes also present high potential. The selection of polymeric material and of encapsulating process depends on the desired product properties, such as solubility, bioavailability, biodegradability, delivery site, particle size, etc. The aim of this project is the development and evaluation of micro/nanostructured systems to modulate the delivery of natural products (ex. crude extracts, semipurified fractions, and isolated compounds), emphasizing different production processes (high pressure homogenisation, ultrasound homogeneization, spray drying and freeze drying, etc), type of excipients and methods for the physicochemical product characterization (size, solubility, permeability, dissolution, electron microscopy, x-ray, thermal analysis, zeta potential, etc). This project will support the implementation and consolidation of a technological platform with the capacity to develop innovative products from natural ingredients, with great potential of industrial application. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PATRÍCIA NIRLANE DA COSTA SOUZA; DÉRICA GONÇALVES TAVARES; CLÁUDIA REGINA FERNANDES SOUZA; MARCELO LUIS LOMBARDI MARTINEZ; WANDERLEY PEREIRA OLIVEIRA; LUÍS HENRIQUE SOUZA GUIMARÃES; PATRÍCIA GOMES CARDOSO. Spray Drying of Coloring Extracts Produced by Fungi Isolated from Brazilian Caves. Brazilian Archives of Biology and Technology, v. 63, 2020-06-12. (11/10333-1)

CLÁUDIA R. F. SOUZA; PATRÍCIA N. FERRAZ-FREITAS; WANDERLEY P. OLIVEIRA. COMPLEXOS DE INCLUSÃO BINÁRIOS, TERNÁRIOS E QUATERNÁRIOS CONTENDO ÓLEO ESSENCIAL DE Lippia sidoides. Química Nova, v. 39, n. 8, p. 979-986, 2016-09-00. (11/10333-1, 14/15905-1)

BENELLI, LUCIMARA; CORTES-ROJAS, DIEGO F.; SOUZA, CLAUDIA REGINA F.; OLIVEIRA, WANDERLEY PEREIRA. Fluid bed drying and agglomeration of phytopharmaceutical compositions. Powder Technology, v. 273, p. 145-153, MAR 2015. (12/09890-6, 12/03427-2, 11/10333-1, 12/00562-6)

CORTES-ROJAS, DIEGO F.; SOUZA, CLAUDIA R. F.; OLIVEIRA, WANDERLEY P.. EFFECTS OF SURFACTANT TYPE ON RETENTION OF BIOACTIVE COMPOUNDS OF SYZYGIUM AROMATICUM IN SPRAY DRIED LIPID BASED SYSTEMS. INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, v. 8, n. 12, p. 5057-5064, DEC 2017. (14/15905-1, 11/10333-1, 12/03427-2, 12/09890-6)

BENELLI, LUCIMARA; OLIVEIRA, WANDERLEY P.. Fluidized bed coating of inert cores with a lipid-based system loaded with a polyphenol-rich Rosmarinus officinalis extract. FOOD AND BIOPRODUCTS PROCESSING, v. 114, p. 216-226, MAR 2019. (12/00562-6, 11/10333-1)

CORTES-ROJAS, DIEGO F.; SOUZA, CLAUDIA R. F.; CHEN, MONG-JEN; HOCHHAUS, GUENTHER; OLIVEIRA, WANDERLEY P.. Effects of lipid formulations on clove extract spray dried powders: comparison of physicochemical properties, storage stability and in vitro intestinal permeation. PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY, v. 23, n. 10, p. 1047-1056, 2018. (12/03427-2, 11/10333-1)

BANKOLE, VICTOR OLORUNTOBA; OSUNGUNNA, MICHAEL OLUWOLE; FERNANDES SOUZA, CLAUDIA REGINA; SALVADOR, SERGIO LUIZ; OLIVEIRA, WANDERLEY PEREIRA. Spray-Dried Proliposomes: an Innovative Method for Encapsulation of Rosmarinus officinalis L. Polyphenols. AAPS PHARMSCITECH, v. 21, n. 5, MAY 18 2020. (14/15905-1, 18/26069-0, 11/10333-1)

BALDIM, IARA; SOUZA, CLAUDIA R. F.; DURAZZO, ALESSANDRA; LUCARINI, MASSIMO; SANTINI, ANTONELLO; SOUTO, ELIANA B.; OLIVEIRA, WANDERLEY P.. Spray-Dried Structured Lipid Carriers for the Loading of Rosmarinus officinalis: New Nutraceutical and Food Preservative. FOODS, v. 9, n. 8, AUG 2020. (18/26069-0, 11/10333-1)

SOUZA, CLAUDIA R. F.; BALDIM, IARA; BANKOLE, VICTOR O.; DA ANA, RAQUEL; DURAZZO, ALESSANDRA; LUCARINI, MASSIMO; CICERO, NICOLA; SANTINI, ANTONELLO; SOUTO, ELIANA B.; OLIVEIRA, WANDERLEY P.. Spouted Bed Dried Rosmarinus officinalis Extract: A Novel Approach for Physicochemical Properties and Antioxidant Activity. AGRICULTURE-BASEL, v. 10, n. 8, AUG 2020. (18/26069-0, 12/03427-2, 11/10333-1)

CORTES-ROJAS, D. F.; SOUZA, C. R. F.; OLIVEIRA, W. P.. Surfactant Mediated Extraction of Antioxidants from Syzygium aromaticum. SEPARATION SCIENCE AND TECHNOLOGY, v. 50, n. 2, p. 207-213, JAN 22 2015. (12/09890-6, 12/03427-2, 11/10333-1)

SECOLIN, VANESSA A.; SOUZA, CLAUDIA R. F.; OLIVEIRA, WANDERLEY P.. Spray drying of lipid-based systems loaded with Camellia sinensis polyphenols. Journal of Liposome Research, v. 27, n. 1, p. 11-20, MAR 2017. (12/13648-6, 12/03427-2, 11/10333-1)

CORTES-ROJAS, DIEGO F.; SOUZA, CLAUDIA R. F.; OLIVEIRA, WANDERLEY P.. Encapsulation of eugenol rich clove extract in solid lipid carriers. Journal of Food Engineering, v. 127, p. 34-42, APR 2014. (12/09890-6, 12/03427-2, 11/10333-1)

BALDIM, IARA; ROSA, DEBORA M.; SOUZA, CLAUDIA R. F.; DA ANA, RAQUEL; DURAZZO, ALESSANDRA; LUCARINI, MASSIMO; SANTINI, ANTONELLO; SOUTO, ELIANA B.; OLIVEIRA, WANDERLEY P.. Factors Affecting the Retention Efficiency and Physicochemical Properties of Spray Dried Lipid Nanoparticles Loaded with Lippia sidoides Essential Oil. BIOMOLECULES, v. 10, n. 5, MAY 2020. (18/26069-0, 11/10333-1)

BALDIM, IARA; OLIVEIRA, WANDERLEY P.; KADIAN, VARSHA; RAO, REKHA; YADAV, NITESH; MAHANT, SHEEFALI; LUCARINI, MASSIMO; DURAZZO, ALESSANDRA; DA ANA, RAQUEL; CAPASSO, RAFFAELE; et al. Natural Ergot Alkaloids in Ocular Pharmacotherapy: Known Molecules for Novel Nanoparticle-Based Delivery Systems. BIOMOLECULES, v. 10, n. 7, JUL 2020. (12/03427-2, 11/10333-1, 18/26069-0)