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Enzymatic synthesis of fatty esters using heating assisted by microwave

Grant number: 11/23194-0
Support type:Regular Research Grants
Duration: April 01, 2012 - March 31, 2014
Field of knowledge:Engineering - Chemical Engineering
Principal Investigator:Paulo Waldir Tardioli
Grantee:Paulo Waldir Tardioli
Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Assoc. researchers:Raquel de Lima Camargo Giordano

Abstract

Lipases are versatile enzymes capable to catalyze hydrolysis, esterification and interesterification reactions. The transesterification of vegetable oils with short-chain alcohols and the esterification of fatty acids with carbohydrates (sucrose, glucose, fructose, etc.) renders products of great commercial interest, such as, biodiesel (methylic or ethylic esters of fatty acids) and biosurfactants (fatty acids of sugars). However, the use of soluble enzymes is not economically attractive due to its high cost of production and its operational fragility. An alternative to diminish these inconveniences is the immobilization of the enzyme on solid supports. This technique is capable to render an insoluble biocatalyst with high stability against different denaturing agents (temperature, pH, organic solvents, and so on). The use of immobilized enzymes allow the reuse of the biocatalyst, the development of continuous process using different reactor configurations, the easy control and interruption of the reaction and the preparation of purer products due to high specificity of enzymes. Particularly, the production of biodiesel from residual oils with high acidity using immobilized lipases is advantageous because the absence of saponification reactions, very common in the alkaline route. In this project will be studied the fatty ester synthesis (biodiesel by transesterification of residual vegetable oils and sugar fatty esters by esterification of fatty acids and carbohydrates) catalyzed by lipases immobilized on bi-functional silica, which will allow to the hydrophobic interaction of the enzyme to the support and its concomitant stabilization for multiple covalent enzyme-support linkages. It is intended to use heating assisted by microwaves to allow faster reactions in order to become economically attractive the use the enzymes. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RAMOS, MARGARITA D.; MIRANDA, LETICIA P.; GIORDANO, RAQUEL L. C.; FERNANDEZ-LAFUENTE, ROBERTO; KOPP, WILLIAM; TARDIOLI, PAULO W. 1,3-Regiospecific Ethanolysis of Soybean Oil Catalyzed by Crosslinked Porcine Pancreas Lipase Aggregates. BIOTECHNOLOGY PROGRESS, v. 34, n. 4, p. 910-920, JUL-AUG 2018. Web of Science Citations: 6.
DE LIMA, LIONETE NUNES; MENDES, ADRIANO AGUIAR; FERNANDEZ-LAFUENTE, ROBERTO; TARDIOLI, PAULO WALDIR; CAMARGO GIORDANO, RAQUEL DE LIMA. Performance of Different Immobilized Lipases in the Syntheses of Short- and Long-Chain Carboxylic Acid Esters by Esterification Reactions in Organic Media. Molecules, v. 23, n. 4 APR 2018. Web of Science Citations: 2.
VESCOVI, VINICIUS; GIORDANO, RAQUEL L. C.; MENDES, ADRIANO A.; TARDIOLI, PAULO W. Immobilized Lipases on Functionalized Silica Particles as Potential Biocatalysts for the Synthesis of Fructose Oleate in an Organic Solvent/Water System. Molecules, v. 22, n. 2 FEB 2017. Web of Science Citations: 16.
VESCOVI, VINICIUS; CARMELLO DOS SANTOS, JESSICA BRAVIN; TARDIOLI, PAULO WALDIR. Porcine pancreatic lipase hydrophobically adsorbed on octyl-silica: A robust biocatalyst for syntheses of xylose fatty acid esters. BIOCATALYSIS AND BIOTRANSFORMATION, v. 35, n. 4, p. 298-305, 2017. Web of Science Citations: 2.
VESCOVI, VINICIUS; KOPP, WILLIAN; GUISAN, JOSE M.; GIORDANO, RAQUEL L. C.; MENDES, ADRIANO A.; TARDIOLI, PAULO W. Improved catalytic properties of Candida antarctica lipase B multi-attached on tailor-made hydrophobic silica containing octyl and multifunctional amino- glutaraldehyde spacer arms. Process Biochemistry, v. 51, n. 12, p. 2055-2066, DEC 2016. Web of Science Citations: 20.
VESCOVI, VINICIUS; ROJAS, MAYERLENIS JIMENEZ; BARALDO, JR., ANDERSON; BOTTA, DANIEL CARRERO; MONTES SANTANA, FELIPE AUGUSTO; COSTA, JULIA PIVA; MACHADO, MARIANA STEFANI; HONDA, VITOR KASUYOSHI; CAMARGO GIORDANO, RAQUEL DE LIMA; TARDIOLI, PAULO WALDIR. Lipase-Catalyzed Production of Biodiesel by Hydrolysis of Waste Cooking Oil Followed by Esterification of Free Fatty Acids. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, v. 93, n. 12, p. 1615-1624, DEC 2016. Web of Science Citations: 8.

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