Evaluation of dietary intake of antioxidants and oxidative stress in individuals with metabolic syndrome

• Grant number: 11/08373-5
• Support Opportunities: Regular Research Grants
• Duration: May 01, 2012 - April 30, 2014
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Camila Renata Corrêa
• Grantee: Camila Renata Corrêa
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Ana Lucia do Anjos Ferreira ; Celia Regina Nogueira ; Vania dos Santos Nunes Nogueira

Abstract

Obesity and overweight are associated with a group of metabolic diseases and vascular diseases which was originally described by Reaven, such as metabolic syndrome (MS). It is characterized by insulin resistance, hyperglycemia, dyslipidemia, abdominal obesity and hypertension. It is estimated that this syndrome reaches a substantial percentage of the population between the fourth and fifth decade of life. Among the factors that characterize metabolic syndrome, abdominal obesity plays a role in the initial development of this disease, with increased caloric intake, adipocyte hypertrophy occurs, yielding the release of reactive oxygen species, leading to a state of oxidative stress. The antioxidant defense system consists of several components, including carotenoids, which are eaten nutritionally, from fruit and vegetables. The literature reports that patients with metabolic syndrome have a deficiency in the consumption of foods rich in antioxidants, with low serum levels. On the other hand, is described that circulating vitamin may be diminished by being consumed by reactive oxygen species that are constantly being released in this disease. Thus, an adequate research on the diet could be one component in the treatment of disease. Based on these, we note that the feeding habits may be related to the oxidative state of these patients. Various methods are used to assess oxidative injury. The highlights are the following individual measurements: oxidation of DNA bases (Comet test), lipid oxidation (MDA level, level of isoprostane) and protein oxidation (carbonylation, nitration). The purpose of this study is to assess, in patients with metabolic syndrome, consumption of antioxidants and their association with indicators of oxidative stress. For this, we analyzed 30 subjects with metabolic syndrome, followed in the outpatient Department of Endocrinology, Medical Clinic of the Hospital of the Medical School of Botucatu, UNESP, Botucatu, SP, conducted by Dr Celia Regina Nogueira and 30 healthy volunteers. The diagnosis of metabolic syndrome will be conducted according to International Diabetes Federation - IDF. Will be excluded from the study patients who have alcoholism, users of antioxidant supplements in the sixty days preceding the study, haematological and albumin changes, changes in liver and kidney function, cancer, smokers, those who make use of statin and patients with diabetes already established. Will be done: anthropometric (weight, height, waist circumference), assessment of food intake record (3 days), plasma levels: fasting glucose, triglycerides, total cholesterol and fractions, liver and kidney functions, blood count, total antioxidant capacity ( TAP), comet assay (DNA); exogenous antioxidants (carotenoids, retinol and tocopherols) concentrations of leptin and adiponectin. (AU)