Evaluation of antioxidant effects of 5-aminosalycylic acid, N-acetylcysteine, sucralfate, curcumin and aqueous extract of Ilex paraguariensis in contente and pattern of tissue expression of intercellular junctions proteins: study in rats

• Grant number: 10/12492-7
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2012 - June 30, 2014
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Carlos Augusto Real Martinez
• Grantee: Carlos Augusto Real Martinez
• Host Institution: Universidade São Francisco (USF). Campus Bragança Paulista. Bragança Paulista , SP, Brazil
• Associated researchers: Marcelo Lima Ribeiro ; Michelle Darrieux Sampaio Bertoncini ; Patrícia de Oliveira Carvalho

Abstract

chronic inflammation in the mucosa of the colon segments devoid of fecal stream. The disease presents clinical, endoscopic and histological features similar to inflammatory bowel disease suggesting common molecular basis. The emergence of the DC is related to a deficiency of short chain fatty acids (SCFAs) in the intestinal lumen, which represent the main energy substrate of the epithelial cells of the colon mucosa. A deficiency of intraluminal SCFAs alter the energy metabolism of cells of the colon mucosa, leading to the formation of oxygen free radicals (ROS). The colonic mucosa is particularly deficient in antioxidant defense systems. The imbalance between increased production of ROS and antioxidant deficiency provoke oxidative stress that cause tissue damage in the defense system that comprise the epithelial barrier of the colonic mucosa. The different systems of intercellular adhesion, mainly represented by systems of tight junctions and adherents junction are in one of the main mechanisms for maintaining the integrity of the epithelium colic. The rupture of the proteins that make up the intercellular junctions systems enables the invasion of the submucosal sterile layer by existing bacteria and antigens in the intestinal lumen, triggering the tissue inflammatory response that characterizes the disease. Although DC is a relatively common disease with increasing incidence worldwide, where oxidative stress due to changes in cellular energy metabolism seems the probable molecular mechanism of epithelial damage, to date not been studied experimentally the effects of substances with antioxidant activity, such as 5-aminosalicylic acid (mesalazine), n-acetylcysteine, sucralfate, curcumin and aqueous extract of Ilex paraguariensis, in tissue content and pattern of expression of protein constituents of of the intercellular adhesion systems in an experimental models of DC. The objective of this study is to evaluate experimentally the effects of mesalazine, N-acetylcysteine, sucralfate, curcumin and aqueous extract of Ilex paraguariensis in the maintenance of content and expression pattern of proteins in systems occlusion junctions and intercellular adhesion in an experimental model of colitis exclusion (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARLOS AUGUSTO REAL MARTINEZ; FABIANO MARCELO DE FABRIS; CAMILA MORAIS GONÇALVES DA SILVA; MURILO ROCHA RODRIGUES; DANIELA TIEMI SATO; MARCELO LIMA RIBEIRO; JOSÉ AIRES PEREIRA. Oxidative stress and changes in the content and pattern of tissue expression of β-catenin protein in diversion colitis. J. Coloproctol. (Rio J.), v. 32, n. 4, p. 343-358, 2012-12-00. (10/12492-7)

CARLOS AUGUSTO REAL MARTINEZ; MURILO ROCHA RODRIGUES; DANIELA TIEMI SATO; CAMILA MORAIS GONÇALVES DA SILVA; DANILO TOSHIO KANNO; ROBERTA LAÍS DOS SANTOS MENDONÇA; JOSÉ AIRES PEREIRA. Avaliação dos efeitos anti-inflamatório e antioxidante do sucralfato na colite de exclusão. J. Coloproctol. (Rio J.), v. 35, n. 2, p. 90-99, 2015-06-00. (10/12492-7)

NONOSE, NILSON; PEREIRA, JOSE AIRES; MOURA MACHADO, PAULO ROBERTO; RODRIGUES, MURILO ROCHA; SATO, DANIELA TIEMI; REAL MARTINEZ, CARLOS AUGUSTO. Oral administration of curcumin (Curcuma longa) can attenuate the neutrophil inflammatory response in zymosan-induced arthritis in rats. Acta Cirurgica Brasileira, v. 29, n. 11, p. 727-734, NOV 2014. (10/12492-7)

REAL MARTINEZ, CARLOS AUGUSTO; KADRI, CALED JAOUDAT; KANNO, DANILO TOSHIO; TIBURCIO ALVES JUNIOR, ANTONIO JOSE; RODRIGUES COY, CLAUDIO SADDY; PEREIRA, JOSE AIRES. Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa. Acta Cirurgica Brasileira, v. 32, n. 1, p. 65+, JAN 2017. (10/12492-7)

GABRIEL BONASSA, CHRISTINY EMMANUELLE; PEREIRA, JOSE AIRES; CASERTA MARYSSAEL DE CAMPOS, FABIO GUILHERME; RODRIGUES, MURILO ROCHA; SATO, DANIELA TIEMI; MENDONCA CHAIM, FELIPE DAVID; REAL MARTINEZ, CARLOS AUGUSTO. Tissue content of sulfomucins and sialomucins in the colonic mucosa, without fecal stream, undergoing daily intervention with sucralfate. Acta Cirurgica Brasileira, v. 30, n. 5, p. 328-338, MAY 2015. (10/12492-7)

JOSÉ AIRES PEREIRA; MURILO ROCHA RODRIGUES; DANIELA TIEMI SATO; PAULO PEDROSO SILVEIRA JÚNIOR; ALICE MOREIRA DIAS; CAMILA GONÇALVES DA SILVA; CARLOS AUGUSTO REAL MARTINEZ. Avaliação dos efeitos da aplicação de enemas com sucralfato em modelo experimental de colite de exclusão. J. Coloproctol. (Rio J.), v. 33, n. 4, p. 182-190, 2013-12-00. (10/12492-7)

CARLOS AUGUSTO REAL MARTINEZ; VITOR PIQUERA DE OLIVEIRA; FABIO GUILHERME CAMPOS; DANILO TOSHIO KANNO; OSCAR ORLANDO ARAYA FERNANDEZ; CALED JAOUDAT KADRI; JOSÉ AIRES PEREIRA. Influência da aplicação de clisteres contendo extrato oleoso de curcumina (Curcuma longa) no conteúdo tecidual de mucinas neutras e ácidas na mucosa cólica sem trânsito intestinal. J. Coloproctol. (Rio J.), v. 37, n. 4, p. 312-319, 2017-12-00. (10/12492-7)

OSCAR ORLANDO ARAYA FERNANDEZ; JOSÉ AIRES PEREIRA; FÁBIO GUILHERME CAMPOS; CAROLINA MARDEGAN ARAYA; GABRIELE ESCOCIA MARINHO; RAFAELA DE SOUZA NOVO; THAIS SILVA DE OLIVEIRA; YARA TINOCO FRANCESCHI; CARLOS AUGUSTO REAL MARTINEZ. [ARTIGO RETRATADO]: AVALIAÇÃO DE ENEMAS COM SUCRALFATO NO CONTEÚDO TECIDUAL DA PROTEINA MUC-2 EM COLITE DE EXCLUSÃO EXPERIMENTAL. ABCD-ARQUIVOS BRASILEIROS DE CIRURGIA DIGESTIVA-BRAZILIAN ARCHIVES OF DIGESTIVE SURGERY, v. 31, n. 3, 2018-08-16. (10/12492-7)

ARAYA FERNANDEZ, OSCAR ORLANDO; PEREIRA, JOSE AIRES; CAMPOS, FABIO GUILHERME; ARAYA, CAROLINA MARDEGAN; MARINHO, GABRIELE ESCOCIA; NOVO, RAFAELA DE SOUZA; DE OLIVEIRA, THAIS SILVA; FRANCESCHI, YARA TINOCO; REAL MARTINEZ, CARLOS AUGUSTO. EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS. ABCD-ARQUIVOS BRASILEIROS DE CIRURGIA DIGESTIVA-BRAZILIAN ARCHIVES OF DIGESTIVE SURGERY, v. 31, n. 3, 2018. (10/12492-7)

ARAYA FERNANDEZ, OSCAR ORLANDO; PEREIRA, JOSE AIRES; CAMPOS, FABIO GUILHERME; ARAYA, CAROLINA MARDEGAN; MARINHO, GABRIELE ESCOCIA; NOVO, RAFAELA DE SOUZA; DE OLIVEIRA, THAIS SILVA; FRANCESCHI, YARA TINOCO; REAL MARTINEZ, CARLOS AUGUSTO. AVALIAÇÃO DE ENEMAS COM SUCRALFATO NO CONTEÚDO TECIDUAL DA PROTEINA MUC-2 EM COLITE DE EXCLUSÃO EXPERIMENTAL. ABCD-ARQUIVOS BRASILEIROS DE CIRURGIA DIGESTIVA-BRAZILIAN ARCHIVES OF DIGESTIVE SURGERY, v. 30, n. 2, p. 132-138, APR-JUN 2017. (10/12492-7)

CHAIM, FELIPE MENDONA; SATO, DANIELA TIEMI; RODRIGUES, MURILO ROCHA; DIAS, ALICE MOREIRA; SILVEIRA JUNIOR, PAULO PEDROSO; PEREIRA, JOSE AIRES; REAL MARTINEZ, CARLOS AUGUSTO. Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis. Acta Cirurgica Brasileira, v. 29, n. 9, p. 544-552, SEP 2014. (10/12492-7)