Experimental study of PCB126 exposure on induction type II Diabetes mellitus

• Grant number: 12/02994-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2012
• End date: May 31, 2014
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Sandra Helena Poliselli Farsky
• Grantee: Sandra Helena Poliselli Farsky
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Diabetes (DM) currently affects 285 million people worldwide. In 2030, an estimated 428 million people will have this disease (IDF, 2010). Since the literature reports a possible association between diabetes and exposure to persistent organic pollutants (POPs) such as polychlorinated biphenyls (PCBs), this project aims to investigate whether exposure to PCB no. 126 triggers endogenous alterations involved in the pathogenesis of type II diabetes. For this purpose, male Wistar rats will be exposed by instillation to the PCB no. 126 0.1, 1 or 10 µg/Kg. Animals exposed to vehicle will be used as a control. Twenty-four hours following the last exposure, animals will be killed for collection of circulating blood and pancreas. In blood samples, will be evaluated: the number of circulating cells, the expression of adhesion molecules in circulating leukocytes by flow cytometry, the plasma levels of circulating adhesion molecules of endothelial activity and the concentration of proinflammatory such IL-1beta, TNF-alpha and IL-6 by ELISA and NO by Griess reaction, biochemical and lipid profile, the insulin levels and basal and glucose tolerance test. In pancreatic tissue samples, will be assessed: the pancreatic islets purity, viability and morphology; the myeloperoxidase activity (MPO); the stereology; proteomics for the profile of inflammatory proteins; the expression of AhR and PPAR receptors by western blot; and leukocyte-endothelial interactions in pancreas microcirculation by intravital microscopy. The results may contribute to the elucidation of the effects caused by PCBs exposure, possibly on observed changes during the development of type II diabetes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOIOLA, RODRIGO AZEVEDO; DOS ANJOS, FABYANA MARIA; SHIMADA, ANA LUCIA; CRUZ, WESLEY SOARES; DREWES, CARINE CRISTIANE; RODRIGUES, STEPHEN FERNANDES; CARDOZO, KARINA HELENA MORAIS; CARVALHO, VALDEMIR MELECHCO; PINTO, ERNANI; FARSKY, SANDRA HELENA. Long-term in vivo polychlorinated biphenyl 126 exposure induces oxidative stress and alters proteomic profile on islets of Langerhans. SCIENTIFIC REPORTS, v. 6, JUN 13 2016. (12/02994-0, 11/09677-8)

RODRIGUES, STEPHEN F.; FIEL, LUANA A.; SHIMADA, ANA L.; PEREIRA, NATALIA R.; GUTERRES, SILVIA S.; POHLMANN, ADRIANA R.; FARSKY, SANDRA H.. Lipid-Core Nanocapsules Act as a Drug Shuttle Through the Blood Brain Barrier and Reduce Glioblastoma After Intravenous or Oral Administration. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v. 12, n. 5, p. 986-1000, MAY 2016. (12/02994-0, 14/05146-6, 11/02438-8, 11/19595-9)

SHIMADA, ANA LUCIA B.; CRUZ, WESLEY S.; LOIOLA, RODRIGO A.; DREWES, CARINE C.; DOERR, FABIANE; FIGUEIREDO, NATALIA G.; PINTO, ERNANI; FARSKY, SANDRA H. P.. Absorption of PCB126 by upper airways impairs G protein-coupled receptor-mediated immune response. SCIENTIFIC REPORTS, v. 5, OCT 9 2015. (12/02994-0, 11/09677-8)