Production of polyclonal antibodies

• Grant number: 03/13387-9
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Duration: August 01, 2004 - January 31, 2009
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Fernanda Alvarez Rojas
• Grantee: Fernanda Alvarez Rojas
• Host Company: Rheabiotec Desenvolvimento, Produção e Comercialização de Produtos de Biotecnologia Ltda
• City: Campinas
• Associated grant(s): 12/51000-8 - Antibodies production for immunochemical methods: market insertion, AP.PIPE3

Abstract

Functionally antibodies can be characterized by their ability to recognize and bind with certain antigens which form antigenic determinants. Taking advantage of the physiological function of antibodies’ humoral immunological defense, these antibodies, when produced in animal serum, can be used as important tools in some biological techniques for scientific research and in tests for diagnostic purposes. Polyclonal antibodies are very valuable in the techniques of immunoprecipitation, immunoblotting, immunohistochemistry and Elisa (enzyme-linked immunosorbent assay). The usual method for obtaining antibodies involves immunization with a preparation of pure or partially purified antigens. Thus, the present project aims to set up a laboratory for the development of the technology for the production of polyclonal antibodies for use in scientific research. It is also intended to make viable the production of these antibodies on a scale with commercial impact, with a substantial reduction in costs and acquisition time compared to those currently imported. (AU)