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Development of nanobiosensors using atomic force spectroscopy: application for detecting pesticides

Abstract

The processing of materials at the nanoscale has been increasingly used in developing supramolecular structures and devices for technological applications. This project addresses mainly biosensors obtained through the functionalization of atomic force microscopy (AFM) tips with organic monolayers, to be applied in the detection of pollutants, especially pesticides. The monolayers will possess well-defined terminal groups, thus allowing molecular recognition. This technique is referred toa as chemical force microscopy (CFM). We shall use functional groups deriving from proteins, thiols and conducting polymers, in addition to antibodies (anti-pesticides) in order to detect immobilized antigens. The emphasis will be given to the possible detection of herbicides that inhibit enzymatic activity through specific interactions, using atomic force spectroscopy (AFS) with the functionalized tips. In parallel to the AFS measurements, we shall employ nanomechanical cantilever sensors, in which the cantilevers are immobilized. This allows small quantities of analytes to be detected via specific interactions. The data obtained with AFS will be compared to results from computational modeling, using molecular dynamics, and with experimental results from surface plasmon resonance. (AU)

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Scientific publications (17)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
IERICH, JESSICA C. M.; OLIVEIRA, GUEDMILLER S.; VIG, ANA C. A.; AMARANTE, ADRIANO M.; FRANCA, EDUARDO F.; LEITE, FABIO L.; MASCARENHAS, YVONNE P. A Computational Protein Structure Refinement of the Yeast Acetohydroxyacid Synthase. Journal of the Brazilian Chemical Society, v. 26, n. 8, p. 1702-1709, AUG 2015. Web of Science Citations: 2.
GARCIA, PAMELA SOTO; DARIO MOREAU, ALBERTO LUIS; MAGALHAES IERICH, JESSICA CRISTIANE; ARAUJO VIG, ANA CAROLINA; HIGA, AKEMI MARTINS; OLIVEIRA, GUEDMILLER S.; ABDALLA, FABIO CAMARGO; HAUSEN, MOEMA; LEITE, FABIO L. A Nanobiosensor Based on 4-Hydroxyphenylpyruvate Dioxygenase Enzyme for Mesotrione Detection. IEEE SENSORS JOURNAL, v. 15, n. 4, p. 2106-2113, APR 2015. Web of Science Citations: 11.
DE MENEZES, APARECIDO JUNIOR; LONGO, ELSON; LEITE, FABIO LIMA; DUFRESNE, ALAIN. Characterization of Cellulose Nanocrystals Grafted with Organic Acid Chloride of Different Sizes. JOURNAL OF RENEWABLE MATERIALS, v. 2, n. 4, p. 306-313, DEC 2014. Web of Science Citations: 5.
MAS, BRUNA ANTUNES; DE MELLO CATTANI, SILVIA MARA; CIPRIANO RANGEL, RITA DE CASSIA; RIBEIRO, GABRIELLE DE ALMEIDA; CRUZ, NILSON CRISTINO; LEITE, FABIO DE LIMA; DE PAULA NASCENTE, PEDRO AUGUSTO; DE REZENDE DUEK, ELIANA APARECIDA. Surface Characterization and Osteoblast-like Cells Culture on Collagen Modified PLDLA Scaffolds. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 17, n. 6, p. 1523-1534, NOV-DEC 2014. Web of Science Citations: 6.
AMARANTE, ADRIANO M.; OLIVEIRA, GUEDMILLER S.; BUENO, CAROLINA C.; CUNHA, RICHARD A.; IERICH, JESSICA C. M.; FREITAS, LUIZ C. G.; FRANCA, EDUARDO F.; OLIVEIRA, JR., OSVALDO N.; LEITE, FABIO L. Modeling the coverage of an AFM tip by enzymes and its application in nanobiosensors. JOURNAL OF MOLECULAR GRAPHICS & MODELLING, v. 53, p. 100-104, SEP 2014. Web of Science Citations: 13.
DA SILVA, ALINE C. N.; DEDA, DAIANA K.; BUENO, CAROLINA C.; MORAES, ARIANA S.; DA ROZ, ALESSANDRA L.; YAMAJI, FABIO M.; PRADO, ROGILENE A.; VIVIANI, VADIM; OLIVEIRA, JR., OSVALDO N.; LEITE, FABIO L. Nanobiosensors Exploiting Specific Interactions Between an Enzyme and Herbicides in Atomic Force Spectroscopy. Journal of Nanoscience and Nanotechnology, v. 14, n. 9, p. 6678-6684, SEP 2014. Web of Science Citations: 8.
STEFFENS, C.; LEITE, F. L.; MANZOLI, A.; SANDOVAL, R. D.; FATIBELLO, O.; HERRMANN, P. S. P. Microcantilever Sensors Coated with a Sensitive Polyaniline Layer for Detecting Volatile Organic Compounds. Journal of Nanoscience and Nanotechnology, v. 14, n. 9, p. 6718-6722, SEP 2014. Web of Science Citations: 7.
BUENO, CAROLINA CASTRO; AMARANTE, ADRIANO MORAES; OLIVEIRA, GUEDMILLER S.; DEDA, DAIANA KOTRA; TESCHKE, OMAR; FRANCA, EDUARDO DE FARIA; LEITE, FABIO L. Nanobiosensor for Diclofop Detection Based on Chemically Modified AFM Probes. IEEE SENSORS JOURNAL, v. 14, n. 5, p. 1467-1475, MAY 2014. Web of Science Citations: 11.
STEFFENS, C.; LEITE, F. L.; MANZOLI, A.; SANDOVAL, R. D.; FATIBELLO, O.; HERRMANN, P. S. P. Microcantilever Sensors Coated With Doped Polyaniline for the Detection of Water Vapor. SCANNING, v. 36, n. 3, p. 311-316, MAY-JUN 2014. Web of Science Citations: 5.
BRUNA ANTUNES MÁS; SILVIA MARA DE MELLO CATTANI; RITA DE CÁSSIA CIPRIANO RANGEL; GABRIELLE DE ALMEIDA RIBEIRO; NILSON CRISTINO CRUZ; FABIO DE LIMA LEITE; PEDRO AUGUSTO DE PAULA NASCENTE; ELIANA APARECIDA DE REZENDE DUEK. Surface characterization and osteoblast-like Cells culture on collagen modified PLDLA scaffolds. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 17, n. 6, p. 1523-1534, Dez. 2014.
STEFFENS, CLARICE; MANZOLI, ALEXANDRA; PASCHOALIN, RAFAELLA TAKEHARA; TIGGEMANN, LIDIA; STEFFENS, JULIANA; TEIXEIRA, ELISANGELA; DE PAULA HERRMANN, PAULO SERGIO. Tracing paper substrate used for development of interdigitated graphite electrode and its application as humidity sensor. Synthetic Metals, v. 183, p. 36-39, NOV 1 2013. Web of Science Citations: 9.
OLIVEIRA, GUEDMILLER S.; LEITE, FABIO L.; AMARANTE, ADRIANO M.; FRANCA, EDUARDO F.; CUNHA, RICHARD A.; BRIGGS, JAMES M.; FREITAS, LUIZ C. G. Molecular modeling of enzyme attachment on AFM probes. JOURNAL OF MOLECULAR GRAPHICS & MODELLING, v. 45, p. 128-136, SEP 2013. Web of Science Citations: 6.
DAIANA K. DEDA; BÁRBARA B. S. PEREIRA; CAROLINA C. BUENO; ALINE N. DA SILVA; GABRIELLE A. RIBEIRO; ADRIANO M. AMARANTE; EDUARDO F. FRANCA; FABIO L. LEITE. The use of functionalized AFM tips as molecular sensors in the detection of pesticides. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 16, n. 3, p. 683-687, Jun. 2013.
DEDA, DAIANA K.; PEREIRA, BARBARA B. S.; BUENO, CAROLINA C.; DA SILVA, ALINE N.; RIBEIRO, GABRIELLE A.; AMARANTE, ADRIANO M.; FRANCA, EDUARDO F.; LEITE, FABIO L. The Use of Functionalized AFM tips as Molecular Sensors in the Detection of Pesticides. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 16, n. 3, p. 683-687, Jun. 2013. Web of Science Citations: 13.
DA SILVA, ALINE C. N.; DEDA, DAIANA K.; DA ROZ, ALESSANDRA L.; PRADO, ROGILENE A.; CARVALHO, CAMILA C.; VIVIANI, VADIM; LEITE, FABIO L. Nanobiosensors Based on Chemically Modified AFM Probes: A Useful Tool for Metsulfuron-Methyl Detection. SENSORS, v. 13, n. 2, p. 1477-1489, FEB 2013. Web of Science Citations: 21.
LEITE, FABIO L.; BUENO, CAROLINA C.; DA ROZ, ALESSANDRA L.; ZIEMATH, ERVINO C.; OLIVEIRA, JR., OSVALDO N. Theoretical Models for Surface Forces and Adhesion and Their Measurement Using Atomic Force Microscopy. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 13, n. 10, p. 12773-12856, OCT 2012. Web of Science Citations: 98.
STEFFENS, CLARICE; LEITE, FABIO L.; BUENO, CAROLINA C.; MANZOLI, ALEXANDRA; DE PAULA HERRMANN, PAULO SERGIO. Atomic Force Microscopy as a Tool Applied to Nano/Biosensors. SENSORS, v. 12, n. 6, p. 8278-8300, JUN 2012. Web of Science Citations: 33.

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