Systems biology of sugarcane bagasse microbial decomposition interactions

• Grant number: 10/18198-3
• Support type: Research Grants - Visiting Researcher Grant - International
• Duration: January 15, 2011 - January 14, 2012
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal researcher: Fábio Márcio Squina
• Grantee: Fábio Márcio Squina
• Visiting researcher: Rolf Alexander Prade
• Visiting researcher institution: Oklahoma State University, United States
• Home Institution: Laboratório Nacional de Luz Síncrotron (LNLS). Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovações (Brasil). Campinas , SP, Brazil
• Associated research grant: 08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome, AP.BIOEN.JP

Abstract

Sugarcane biomass (bagasse) along with other leftover biomass such as corn and sorghum stover is present in large quantities alongside large-scale biofuel-refineries. The utilization of this biomass as a source of fermentable sugars (by degradation of cellulose and hemicellulose) produce second-generation biofuels, which are high priority development items in most BioEnergy Technology Programs. In nature dead biomass is entirely recycled by microbial systems. This means that recalcitrant polymers are decomposed into its utilizable sugar and phenolic components that promote microbial growth producing CO2 and H2O, which in turn is again fixed into sugars and converted into polysaccharides. We hypothesize that for this natural recycling process to take place an enzyme system, rather than individual enzymes are necessary to accomplish complete degradation. Thus, we propose to look at the enzyme system in nature (decomposing sugarcane bagasse) as a whole, determine its genetic basis and reconstruct a heterologous system useable in commercial operations. The sugarcane proteo-genomics project is a collaboration, between Oklahoma State University and the Brazilian Bioethanol Science and Technology Laboratory (CBTE) and interfaces with several local projects such as the Oklahoma Biofuels Center (Elshahed, OBC) and ongoing projects funded by the Department of Energy, United States Department of Agriculture and others. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORREA, THAMY L. R.; FRANCO CAIRO, JOAO PAULO L.; COTA, JUNIO; DAMASIO, ANDRE; OLIVEIRA, LEANDRO C.; SQUINA, FABIO M. A novel mechanism of beta-glucosidase stimulation through a monosaccharide binding-induced conformational change. International Journal of Biological Macromolecules, v. 166, p. 1188-1196, JAN 1 2021. Web of Science Citations: 0.

MANDELLI, F.; COUGER, M. B.; PAIXAO, D. A. A.; MACHADO, C. B.; CARNIELLI, C. M.; ARICETTI, J. A.; POLIKARPOV, I.; PRADE, R.; CALDANA, C.; PAES LEME, A. F.; MERCADANTE, A. Z.; RIANO-PACHON, D. M.; SQUINA, FABIO MARCIO. Thermal adaptation strategies of the extremophile bacterium Thermus filiformis based on multi-omics analysis. EXTREMOPHILES, v. 21, n. 4, p. 775-788, JUL 2017. Web of Science Citations: 7.

COTA, JUNIO; CORREA, THAMY L. R.; DAMASIO, ANDRE R. L.; DIOGO, JOSE A.; HOFFMAM, ZAIRA B.; GARCIA, WANIUS; OLIVEIRA, LEANDRO C.; PRADE, ROLF A.; SQUINA, FABIO M. Comparative analysis of three hyperthermophilic GH1 and GH3 family members with industrial potential. NEW BIOTECHNOLOGY, v. 32, n. 1, p. 13-20, JAN 25 2015. Web of Science Citations: 22.

SEGATO, FERNANDO; DAMASIO, ANDRE R. L.; DE LUCAS, ROSYMAR C.; SQUINA, FABIO M.; PRADE, ROLF A. Genomics Review of Holocellulose Deconstruction by Aspergilli. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, v. 78, n. 4, p. 588-613, DEC 2014. Web of Science Citations: 33.

GONCALVES, T. A.; DAMASIO, A. R. L.; SEGATO, F.; ALVAREZ, T. M.; BRAGATTO, J.; BRENELLI, L. B.; CITADINI, A. P. S.; MURAKAMI, M. T.; RULLER, R.; PAES LEME, A. F.; PRADE, R. A.; SQUINA, F. M. Functional characterization and synergic action of fungal xylanase and arabinofuranosidase for production of xylooligosaccharides. Bioresource Technology, v. 119, p. 293-299, SEP 2012. Web of Science Citations: 40.

SEGATO, FERNANDO; DAMASIO, ANDRE R. L.; GONCALVES, THIAGO A.; DE LUCAS, ROSYMAR C.; SQUINA, FABIO M.; DECKER, STEPHEN R.; PRADE, ROLF A. High-yield secretion of multiple client proteins in Aspergillus. Enzyme and Microbial Technology, v. 51, n. 2, p. 100-106, JUL 15 2012. Web of Science Citations: 37.

SEGATO, FERNANDO; DAMASIO, ANDRE R. L.; GONCALVES, THIAGO AUGUSTO; MURAKAMI, MARIO T.; SQUINA, FABIO M.; POLIZELI, MARIA DE LOURDES T. M.; MORT, ANDREW J.; PRADE, ROLF A. Two structurally discrete GH7-cellobiohydrolases compete for the same cellulosic substrate fiber. BIOTECHNOLOGY FOR BIOFUELS, v. 5, APR 11 2012. Web of Science Citations: 12.