Study of the self-assembly of single stranded DNA on modified metallic surfaces

• Grant number: 12/05734-0
• Support Opportunities: Research Grants - Visiting Researcher Grant - Brazil
• Start date: August 01, 2012
• End date: July 31, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Sidney José Lima Ribeiro
• Grantee: Sidney José Lima Ribeiro
• Visiting researcher: Frederico Guilherme de Carvalho Cunha
• Visiting researcher institution: Universidade Federal de Sergipe (UFS). Centro de Ciências Exatas e Tecnologia (CCET) , Brazil
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• City of the host institution: Araraquara

Abstract

The main purpose of this proposal is the study of the adsorptionprocess of single stranded DNA of Leishmania (Vianna) Brasiliensis ongold electrodes. As a result of this study we expect to create thenecessary conditions for the production of a new detection protocol ofLeishmaniosis through the blood serum as well as the determination ofthe species that caused the contagion. The study will be based on thedetermination of the self assembly process of the single stranded DNAmodified by a thiol group adsorbed on single crystalline goldelectrodes using Scanning Tunneling Microscopy. The hybridizationprocess will be monitored through the use of Surface Enhanced RamanSpectroscopy on roughened gold electrodes. (AU)