Morphofunctional and genomic evaluation of melanocytes culture from patients with facial melasma

• Grant number: 12/09233-5
• Support Opportunities: Regular Research Grants
• Duration: October 01, 2012 - September 30, 2015
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Hélio Amante Miot
• Grantee: Hélio Amante Miot
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Luciane Donida Bartoli Miot ; Mariangela Esther Alencar Marques ; Silvya Stuchi Maria-Engler

Abstract

Melasma is a common disorder of hyperpigmentation, characterized by symmetric brown patches on skin exposed to sun. It's more frequent in the face, forehead and temples. Ultraviolet radiation (UVR), genetic influence and endocrine factors such as pregnancy, oral contraceptives, thyroid dysfunction and some medications and cosmetics are some of etiological factors. However none of them can be considered solely responsible for the development of the disease. The affected skin with melasma has some histological differences of healthy skin, such as increased melanogenic activity. The melasma pathogenesis is still unknown, while requiring a better characterization of the different behaviors of skin lesions and the adjacent, once they receive similar influences. The complexity of the skin, their intercellular interaction and presence of a small number of melanocytes per histologic section, hinder conclusions about what cell is the effector of disorder and their behavior. This study proposes a morphologic and functional evaluation of melanocytes primary culture from biopsies of patients with a facial melasma by transmission electron microscopy and genomics by PCR-array, and also the development a model that allows the investigation and determination of the effector cell of melasma, independently of other skin cells, allowing the progression of knowledge on melasma physiopathology. Additionally, we will investigate semiquantitatively the expression of peptides linked to melanogenesis in melasma (MC1-R, ±-MSH, COX-2, nuclear receptors of progesterone e estrogen-²) by double labeling immunofluorescence. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAVALCANTE ESPOSITO, ANA CLAUDIA; BRIANEZI, GABRIELLI; DE SOUZA, NATHALIA PEREIRA; SANTOS, DANIELA CARVALHO; BARTOLI MIOT, LUCIANE DONIDA; MIOT, HELLO AMANTE. Ultrastructural characterization of damage in the basement membrane of facial melasma. ARCHIVES OF DERMATOLOGICAL RESEARCH, v. 312, n. 3, p. 223-227, APR 2020. (12/09233-5)

ESPOSITO, A. C. C.; BRIANEZI, G.; DE SOUZA, N. P.; MIOT, L. D. B.; MARQUES, M. E. A.; MIOT, H. A.. Exploring pathways for sustained melanogenesis in facial melasma: an immunofluorescence study. International Journal of Cosmetic Science, v. 40, n. 4, p. 420-424, AUG 2018. (12/05004-1, 12/09233-5)

ANA CLÁUDIA CAVALCANTE ESPÓSITO; GABRIELLI BRIANEZI; LUCIANE DONIDA BARTOLI MIOT; HÉLIO AMANTE MIOT. Fibroblast morphology, growth rate and gene expression in facial melasma. ANAIS BRASILEIROS DE DERMATOLOGIA, v. 97, n. 5, p. 575-582, 2022-09-30. (12/05004-1, 12/09233-5)

CAVALCANTE ESPOSITO, ANA CLAUDIA; DE SOUZA, NATHALIA PEREIRA; BARTOLI MIOT, LUCIANE DONIDA; MIOT, HELIO AMANTE. Deficit in autophagy: A possible mechanism involved in melanocyte hyperfunction in melasma. INDIAN JOURNAL OF DERMATOLOGY VENEREOLOGY & LEPROLOGY, v. 87, n. 4, p. 585-586, JUL-AUG 2021. (12/09233-5, 12/05004-1)