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Study of the genome and metabolome of the Haemophilus influenzae strains causing the Brazilian purpuric fever

Grant number: 12/15046-3
Support type:Regular Research Grants
Duration: October 01, 2012 - September 30, 2014
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal researcher:Marcelo Lancellotti
Grantee:Marcelo Lancellotti
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


In the 80 years the Brazilian purpuric fever epidemic outbreaks swept through the region of Ribeirao Preto in São Paulo, causing several deaths and having a bacterium as the etiologic agent. This bacterium was only related to cases of bacterial conjunctivitis (where often associated with Haemophilus influenzae biotype aegyptius) and suddenly showed a strain of Haemophilus influenzae septicemic, which is a rare event. Such outbreaks, for unknown reasons, were happily quenched, probably due to the immediate clinical attention received cases however, the question of which one (s) number (s) factor (s) of virulence of this bacterium are involved in the pathophysiology of Brazilian purpuric fever , or where they come from the genes responsible for these virulence factors have not been well elucidated. Later, at the end of 90 years much has been said about horizontal gene transfer between major human pathogenic bacteria to upper respiratory tract has its main entrance as Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae, especially after the publication of Swartley et al. (1997) on the capsule switching in N. meningitidis. In 1998, Kroll et al. described the genetic transfers between the three main pathogens described above and hypothesizes that Haemophilus influenzae biotype aegyptius Brazilian purpuric fever causes, is a result of gene transfer of N. meningitidis (causing septic shock) with strains of H. influenzae (which does not cause disseminated intravascular clotting process). We propose a better understanding of these genetic lineages (through sequencing the whole genome of two of them and make any analysis of protein expression in different culture conditions in vitro and in vivo. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
JOSÉ DIOGO DE OLIVEIRA; CARLOS FERNANDO MACEDO; JOÃO PAULO DE OLIVEIRA GUARNIERI; THAÍS HOLTZ THEIZEN; DAISY MACHADO; MARCELO LANCELLOTTI. Influence of environmental dust in transformation capacity of Streptococcus pneumoniae. GENETICS AND MOLECULAR BIOLOGY, v. 44, n. 1, p. -, 2021. Web of Science Citations: 0.
RAFAELLA FABIANA CARNEIRO PEREIRA; THAIS HOLTZ THEIZEN; DAISY MACHADO; JOÃO PAULO DE OLIVEIRA GUARNIERI; GABRIEL PICCIRILLO GOMIDE; LUCIANA MARIA DE HOLLANDA; MARCELO LANCELLOTTI. Analysis of potential virulence genes and competence to transformation in Haemophilus influenzae biotype aegyptius associated with Brazilian Purpuric Fever. GENETICS AND MOLECULAR BIOLOGY, v. 44, n. 1, p. -, 2021. Web of Science Citations: 0.
PEREIRA, RAFAELLA F. C.; MOFATTO, LUCIANA S.; SILVA, ANA C. A.; ALVES, DANILO A.; MACHADO, DAISY; THEIZEN, THAIS H.; PEREIRA, GONCALO A. G.; LEVY, CARLOS E.; DE HOLLANDA, LUCIANA M.; CARAZZOLLE, MARCELO F.; LANCELLOTTI, MARCELO. Draft Whole-Genome Sequences of Haemophilus influenzae Biogroup aegyptius Strains Isolated from Five Brazilian Purpuric Fever Cases and One Conjunctivitis Case. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 8, n. 30 JUL 2019. Web of Science Citations: 1.
VARELA, JULIA NOGUEIRA; KRAEHENBUEHL AMSTALDEN, MARIA CECILIA; CARNEIRO PEREIRA, RAFAELLA FABIANA; DE HOLLANDA, LUCIANA MARIA; CERAGIOLI, HELDER JOSE; BARANAUSKAS, VITOR; LANCELLOTTI, MARCELO. Haemophilus influenzae porine ompP2 gene transfer mediated by graphene oxide nanoparticles with effects on transformation process and virulence bacterial capacity. JOURNAL OF NANOBIOTECHNOLOGY, v. 12, APR 16 2014. Web of Science Citations: 10.

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Filed patent(s) as a result of this research project

VACINA PARA HAEMOPHILUS AEGYPTIUS E USO BR1020160285070 - Universidade Estadual de Campinas (UNICAMP) . Thais Holtz Theizen; Rafaella Fabiana Carneiro Pereira; Daisy Machado; Marcelo Lancellott - December 2016, 05

VACINA PARA Haemophilus aegyptius E USO PCT/BR2017/000135 - Universidade Estadual de Campinas (UNICAMP) . Marcelo Lancellotti (FCF); Rafaella Fabiana Carneiro Pereira (IB); Daisy Machado (IB); Thaís Holtz Theizen (IB) - November 2017, 17