Taxonomy, phylogeny, biogeography, and evolution of Neopasiphaeinae bees (Hymenoptera: Colletidae) using molecular and morphological data

• Grant number: 11/09477-9
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: November 01, 2012
• End date: October 31, 2016
• Field of knowledge: Biological Sciences - Zoology - Taxonomy of Recent Groups
• Principal Investigator: Eduardo Andrade Botelho de Almeida
• Grantee: Eduardo Andrade Botelho de Almeida
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 15/15347-1 - Phylogenetic relationships and comparative internal morphology of bees (Hymenoptera: Apoidea: Anthophila), BP.DR; 13/25936-9 - Comparative phylogeography of two neotropical pollinating fig wasp species, BP.PD; 12/24335-9 - Wing morphology of Neopasiphaeinae bees: comparisons between traditional and morphometric methods for a phylogenetic investigation of form, BP.IC

Abstract

Neopasiphaeinae comprises about 420 species, distributed in the Neotropical and Australian Regions. This subfamily is morphologically diverse and it is known for its specialization in host-plant associations, making this group of bees very attractive from the ecological-evolutionary point of view. Regrettably, limitations in our understanding of the phylogenetic relationships among its genera, and additional problems of their taxonomy and systematics hinder further advances in the research of Neopasiphaeinae. The main goal of this project is to make progress on the understanding of phylogenetic relationships within Neopasiphaeinae, which will be investigated using a combination of morphological and molecular data. The proposed taxon-sampling will include a set of at least 67 terminal species, comprising representatives of all 26 Neotropical neopasiphaeine genera, as well as 10 Australian genera. Outgroup taxa will include representatives of the colletid subfamilies Callomelittinae, Colletinae, and Diphaglossinae, in addition to two species of Stenotritidae. Detailed anatomical work of adults, including male and female terminalia, and internal skeletal features will be the basis for the preparation of a morphological data-matrix. The molecular component of the dataset will include DNA sequences of three nuclear and one mitochondrial gene-loci (Elongation Factor 1-alpha [EF-1a F2], LW-Rhodopsin, and 28S rRNA), plus one mitochondrial gene (the region of Citochrome Oxidase 1 [COI] sampled for the Bee Barcode of Life Initiative "Bee-BOL"). These genes include a combination of regions with distinct substitution rates, which have been very useful to phylogenetic studies pursued with bees and other insects. Phylogenetic results will allow for the following specific goals to be sought: (1) re-evaluation of the taxonomy of Neopasiphaeinae and the limits of its component genera, as well as taxonomic revisions of four Neotropical genera; (2) biogeographic inferences on the history of Neopasiphaeinae; (3) assessment of absolute ages of divergence (molecular dating) for the lineages comprised in the subfamily; (4) investigation of morphological evolution of neopasiphaeine bees and possible relationships to their host-plant shifts through time; (5) devising of integrative macroevolutionary scenarios to explain the aspects of the history of Neopasiphaeinae and its importance to the assembly of the Neotropical bee community through the Cenozoic. Furthermore, an important outcome of this project will be the beginning of a research group of insect molecular systematics at the Biology Department, where this research will be conducted. This novel approach will be pursued concurrently with the strengthening of fields that have a long-term tradition at the institution, particularly comparative morphology and taxonomy, in addition to the expansion of Coleção Camargo in terms of its geographic as well as taxonomic representativeness. (AU)

Scientific publications

(28)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ELIAS, LARISSA GALANTE; KJELLBERG, FINN; ANTONIOLLI FARACHE, FERNANDO HENRIQUE; ALMEIDA, EDUARDO A. B.; RASPLUS, JEAN-YVES; CRUAUD, ASTRID; PENG, YAN-QIONG; YANG, DA-RONG; SANTINELO PEREIRA, RODRIGO AUGUSTO. Ovipositor morphology correlates with life history evolution in agaonid fig wasps. ACTA OECOLOGICA-INTERNATIONAL JOURNAL OF ECOLOGY, v. 90, n. SI, p. 109-116, JUL 2018. (11/09477-9, 13/01918-1, 15/25417-7, 15/06430-2)

LUCENA, DAERCIO A. A.; ALMEIDA, EDUARDO A. B.. Morphology and Bayesian tip-dating recover deep Cretaceous-age divergences among major chrysidid lineages (Hymenoptera: Chrysididae). ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, v. 194, n. 1, p. 36-79, JAN 2022. (18/09666-5, 15/12326-3, 11/09477-9, 17/25081-4)

ALMEIDA, EDUARDO A. B.; QUINTEIRO, FABIO B.. Two continents and two names for a Neotropical colletid bee species (Hymenoptera: Colletidae: Neopasiphaeinae): Hoplocolletes ventralis (Friese, 1924). PeerJ, v. 3, OCT 20 2015. (11/09477-9)

ALMEIDA, E. A. B.; PORTO, D. S.. Investigating Eusociality in Bees while Trusting the Uncertainty. Sociobiology, v. 61, n. 4, p. 355-368, DEC 2014. (12/22261-8, 14/10090-0, 11/09477-9)

ALMEIDA, EDUARDO A. B.; GIBRAN, NADIA S.. Taxonomy of neopasiphaeine bees: review of Tetraglossula Ogloblin, 1948 (Hymenoptera: Colletidae). Zootaxa, v. 4303, n. 4, p. 521-544, AUG 11 2017. (11/09477-9)

MENEZES, RODOLPHO S. T.; BARDELLA, VANESSA B.; CABRAL-DE-MELLO, DIOGO C.; LUCENA, DAERCIO A. A.; ALMEIDA, EDUARDO A. B.. Are the TTAGG and TTAGGG telomeric repeats phylogenetically conserved in aculeate Hymenoptera?. NATURWISSENSCHAFTEN, v. 104, n. 9-10, OCT 2017. (16/21098-7, 15/02432-0, 15/12326-3, 11/09477-9)

HASEYAMA, KIRSTERN L. F.; WIEGMANN, BRIAN M.; ALMEIDA, EDUARDO A. B.; DE CARVALHO, CLAUDIO J. B.. Say goodbye to tribes in the new house fly classification: A new molecular phylogenetic analysis and an updated biogeographical narrative for the Muscidae (Diptera). Molecular Phylogenetics and Evolution, v. 89, p. 1-12, AUG 2015. (11/09477-9)

LUCENA, DAERCIO A. A.; KIMSEY, LYNN S.; ALMEIDA, EDUARDO A. B.. Phylogenetic relationships and biogeography of the Ipsiura cuckoo wasps (Hymenoptera: Chrysididae). Systematic Entomology, v. 44, n. 1, p. 192-210, JAN 2019. (15/12326-3, 13/01195-0, 11/09477-9, 14/12407-0)

ALMEIDA, EDUARDO A. B.; COSTA, ANNE M.; TAVARES FILHO, JOSE AMILCAR; ZICHINELLI, MARCELA M. P.; QUINTEIRO, FABIO B.. Illustrated catalogue of type specimens of insects (Hexapoda) at Colecao Entomologica ``Prof. JMFCamargo{''} (RPSP), Universidade de Sao Paulo, Brazil. Zootaxa, v. 4842, n. 1, p. 1-204, AUG 31 2020. (18/09666-5, 11/09477-9)

OLIVEIRA, FABIO G. L.; CIPOLA, NIKOLAS G.; ALMEIDA, EDUARDO A. B.. Systematics and biogeography of Salina MacGillivray (Collembola: Entomobryoidea), with emphasis on the species groups in the New World. Insect Systematics and Evolution, v. 51, n. 1, p. 81-138, DEC 2020. (13/26335-9, 16/14847-3, 11/09477-9)

DOS SANTOS, CHARLES FERNANDO; HALINSKI, ROSANA; DE SOUZA DOS SANTOS, PATRICK DOUGLAS; ALMEIDA, EDUARDO A. B.; BLOCHTEIN, BETINA. Looking beyond the flowers: associations of stingless bees with sap-sucking insects. NATURWISSENSCHAFTEN, v. 106, n. 3-4, APR 2019. (11/09477-9)

MENEZES, RODOLPHO S. T.; GAZONI, THIAGO; COSTA, MARCO A.. Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera. Biological Journal of the Linnean Society, v. 126, n. 4, p. 925-935, APR 2019. (15/02432-0, 16/21098-7, 11/09477-9)

OLIVEIRA, FABIO G. L.; GREENSLADE, PENELOPE; BELLINI, BRUNO CAVALCANTE. Four new species of Plumachaetas Salmon (Collembola: Entomobryoidea) from Oceania with comments on the genus and a key to species. Zootaxa, v. 4706, n. 1, p. 121-146, DEC 6 2019. (13/26335-9, 16/14847-3, 11/09477-9)

STEFANELLO, FABIANO; MENEZES, RODOLPHO S. T.; RIBEIRO, I, JOSE RICARDO; ALMEIDA, EDUARDO A. B.. Widespread Gene Flow Model Explains the Genetic-Morphological Variation in a Giant Water Bug Species Under Fine-Scale Spatial Sampling. Annals of the Entomological Society of America, v. 113, n. 3, p. 160-170, MAY 2020. (11/09477-9, 15/07166-7, 16/21098-7, 16/12720-6, 18/09666-5)

PORTO, DIEGO S.; ALMEIDA, EDUARDO A. B.; VILHELMSEN, LARS. Comparative morphology of internal structures of the mesosoma of bees with an emphasis on the corbiculate clade (Apidae: Apini). ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, v. 179, n. 2, p. 35-pg., 2017-02-01. (11/09477-9, 12/22261-8, 14/10090-0)

ELIAS, LARISSA GALANTE; KJELLBERG, FINN; ANTONIOLLI FARACHE, FERNANDO HENRIQUE; ALMEIDA, EDUARDO A. B.; RASPLUS, JEAN-YVES; CRUAUD, ASTRID; PENG, YAN-QIONG; YANG, DA-RONG; SANTINELO PEREIRA, RODRIGO AUGUSTO. Ovipositor morphology correlates with life history evolution in agaonid fig wasps. ACTA OECOLOGICA-INTERNATIONAL JOURNAL OF ECOLOGY, v. 90, p. 8-pg., 2018-07-01. (15/25417-7, 11/09477-9, 15/06430-2, 13/01918-1)

PORTO, DIEGO S.; ALMEIDA, EDUARDO A. B.; VILHELMSEN, LARS. Comparative morphology of internal structures of the mesosoma of bees with an emphasis on the corbiculate clade (Apidae: Apini). Zoological Journal of the Linnean Society, v. 179, n. 2, p. 303-337, FEB 1 2017. (12/22261-8, 11/09477-9, 14/10090-0)

LUCENA, DAERCIO. The uncommon genus Hedychridium (Hymenoptera: Chrysididae) in South America: new species and first record for Brazil. Journal of Natural History, v. 52, n. 5-6, p. 351-359, 2018. (15/12326-3, 11/09477-9)

LOPES, ROGERIO B.; MENEZES, RODOLPHO S. T.. Synoeca ilheensis sp nov., a new social wasp (Hymenoptera, Vespidae, Polistinae) from Brazilian lowland Atlantic Forest. Zootaxa, v. 4300, n. 3, p. 445-450, AUG 3 2017. (16/21098-7, 15/02432-0, 11/09477-9)

BOSSERT, SILAS; MURRAY, ELIZABETH; ALMEIDA, EDUARDO; BRADY, SEAN; BLAIMER, BONNIE; DANFORTH, BRYAN. Combining transcriptomes and ultraconserved elements to illuminate the phylogeny of Apidae. Molecular Phylogenetics and Evolution, v. 130, p. 121-131, JAN 2019. (11/09477-9)

DE LIMA OLIVEIRA, FABIO GONCALVES; CIPOLA, NIKOLAS GIOIA. Two new species of Salina MacGillivray (Collembola, Paronellidae) with rectangular mucro from South America. Revista Brasileira de Entomologia, v. 60, n. 2, p. 128-136, APR-JUN 2016. (11/09477-9, 13/26335-9)

LUCENA, DAERCIO A. A.; GOMES, RAYANE S.. New species of Elampus Spinola, 1806 (Hymenoptera: Chrysididae), with a key to the Neotropical species of the genus. Zootaxa, v. 4117, n. 4, p. 555-566, JUN 2 2016. (13/01195-0, 11/09477-9)

CIPOLA, NIKOLAS GIOIA; DE LIMA OLIVEIRA, FABIO GONCALVES. A new species of Campylothorax Schott, 1893 (Collembola, Paronellidae) from Brazilian Amazon, with an identification key to the genus. Zootaxa, v. 4109, n. 4, p. 487-495, MAY 10 2016. (13/26335-9, 11/09477-9)

LUCENA, DAERCIO A. A.; KIMSEY, LYNN S.; ALMEIDA, EDUARDO A. B.. The Neotropical cuckoo wasp genus Ipsiura Linsenmaier, 1959 ( Hymenoptera: Chrysididae): revision of the species occurring in Brazil. Zootaxa, v. 4165, n. 1, p. 1+, SEP 13 2016. (13/01195-0, 11/09477-9, 14/12407-0)

PORTO, DIEGO S.; VILHELMSEN, LARS; ALMEIDA, EDUARDO A. B.. Comparative morphology of the mandibles and head structures of corbiculate bees (Hymenoptera: Apidae: Apini). Systematic Entomology, v. 41, n. 2, p. 339-368, APR 2016. (12/22261-8, 14/10090-0, 11/09477-9)

DIEGO SASSO PORTO; GABRIEL A.R. MELO; EDUARDO A.B. ALMEIDA. Clearing and dissecting insects for internal skeletal morphological research with particular reference to bees. Revista Brasileira de Entomologia, v. 60, n. 1, p. 109-113, 2016-03-00. (11/09477-9, 12/22261-8, 14/10090-0)

ALMEIDA, EDUARDO A. B.; PACKER, LAURENCE; MELO, GABRIEL A. R.; DANFORTH, BRYAN N.; CARDINAL, SOPHIE C.; QUINTEIRO, FABIO B.; PIE, MARCIO R.. The diversification of neopasiphaeine bees during the Cenozoic (Hymenoptera: Colletidae). ZOOLOGICA SCRIPTA, v. 48, n. 2, p. 226-242, MAR 2019. (12/08479-0, 11/09477-9)

PORTO, DIEGO S.; ALMEIDA, EDUARDO A. B.. A comparative study of the pharyngeal plate of Apoidea (Hymenoptera: Aculeata), with implications for the understanding of phylogenetic relationships of bees. ARTHROPOD STRUCTURE & DEVELOPMENT, v. 50, p. 64-77, MAY 2019. (18/09666-5, 15/15347-1, 11/09477-9)