Environmental contamination by protozoa Giardia spp. and Cryptosporidium spp. and Ascaris suum: challenges of detection, removal and inactivation of infective forms

• Grant number: 12/50522-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: January 01, 2013
• End date: December 31, 2018
• Field of knowledge: Engineering - Sanitary Engineering - Environmental Sanitation
• Principal Investigator: Regina Maura Bueno Franco
• Grantee: Regina Maura Bueno Franco
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Pesquisadores principais: José Roberto Guimarães ; Luiz Antonio Daniel
• Associated scholarship(s): 14/21602-1 - PMA vital dye efficiency and PCR evaluation in determining Giardia spp. cysts and Cryptosporidium spp. oocysts inactivation: comparative study with animal infectivity, BP.PD; 14/17997-0 - Characterization of Cryptosporidium spp. and Giardia spp. species in waters collected for urban supply in the Municipality of Campinas, São Paulo, Brazil, BP.IC; 13/26211-8 - Molecular Characterization of Cryptosporidium spp and Giardia spp species in urban water catchment in Campinas, Sao Paulo, Brazil, BP.IC; 13/08192-6 - Removal and inactivation of Giardia spp. and Cryptosporidium spp. in drinking water with high turbidity, using flotation, employing aluminum hydroxychloride and ozone, BP.MS

Abstract

The transmission of waterborne diseases related to water supply for human consumption has been recorded even in developed countries. The authorities' attention has focused on actions related to detection and control of protozoa Cryptosporidium spp. and Giardia spp. since several outbreaks caused by these microorganisms have been reported in recent decades. The main source of water contamination by these pathogens is the disposal of domestic sewage (treated or not) into water that is commonly used as source of supply. In Brazil, the services of collecting, transporting and treating sewage are still poor. The fact has spawned the emergence of several waterborne outbreaks for protozoa in the country. Despite of the advances in the Brazilian research in relation to the detection and treatment of pathogens in the water, there are still many uncertainties related to the efficiency of disinfection, disposal of generated sludge in water treatment and sewage systems, and knowledge of particular species in Brazil and its potential sources of infection. Due to the existing problem, this project aims to assess the environmental contamination generated by the protozoa Giardia spp. and Cryptosporidium spp. and eggs of Ascaris suum, considering the challenges of detection, removal and inactivation of infectious forms. The successful development of this project will suggest methodological basis: i) to evaluate the occurrence of Giardia cysts and Ascaris suum eggs in the soil, ii) to study the removal of protozoa in drinking water and the disposal alternatives of generated sludge after treatment, iii) to evaluate the removal of protozoa in sewage treatment and effluent disposal in surface water, and iv) to guide the best method to assess infectivity caused by protozoa. (AU)

Scientific publications

(26)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAMMARRO SILVA, KAMILA JESSIE; SABOGAL-PAZ, LYDA PATRICIA. Giardia spp. cysts and Cryptosporidium spp. oocysts in drinking water treatment residues: comparison of recovery methods for quantity assessment. ENVIRONMENTAL TECHNOLOGY, FEB 2020. (12/50522-0)

MEDEIROS, RAPHAEL CORREA; SAMMARRO SILVA, KAMILA JESSIE; DANIEL, LUIZ ANTONIO. astewater treatment performance in microbiological removal and (oo)cyst viability assessed comparatively to fluorescence deca. ENVIRONMENTAL TECHNOLOGY, v. 43, n. 7, AUG 2020. (12/50522-0, 10/07902-1, 11/08653-8)

DOS SANTOS, P. R.; DANIEL, L. A.. A review: organic matter and ammonia removal by biological activated carbon filtration for water and wastewater treatment. INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY, v. 17, n. 1, OCT 2019. (12/50522-0)

GIGLIO, GUILHERME LELIS; SABOGAL-PAZ, LYDA PATRICIA. Performance comparison of three methods for detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in drinking-water treatment sludge. ENVIRONMENTAL MONITORING AND ASSESSMENT, v. 190, n. 11, NOV 2018. (12/50522-0)

MEDEIROS, RAPHAEL CORREA; DANIEL, LUIZ ANTONIO. Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge. ACTA SCIENTIARUM-TECHNOLOGY, v. 40, 2018. (12/50522-0, 10/07902-1, 11/08653-8)

SAMMARRO SILVA, KAMILA JESSIE; SABOGAL-PAZ, LYDA PATRICIA. nalytical challenges and perspectives of assessing viability of Giardia muris cysts and Cryptosporidium parvum oocysts by live/dead simultaneous stainin. ENVIRONMENTAL TECHNOLOGY, v. 43, n. 1, p. 60-69, JAN 2 2022. (12/50522-0)

DE OLIVEIRA, GABRIELA LAILA; DANIEL, LUIZ ANTONIO. Removal of Giardia spp. cysts and Cryptosporididum spp. oocysts from anaerobic effluent by dissolved air flotation. ENVIRONMENTAL TECHNOLOGY, v. 42, n. 1, JUN 2019. (12/50522-0)

DOS SANTOS, PRISCILA RIBEIRO; DANIEL, LUIZ ANTONIO. Occurrence and removal of Giardia spp. cysts and Cryptosporidium spp. oocysts from a municipal wastewater treatment plant in Brazil. ENVIRONMENTAL TECHNOLOGY, v. 38, n. 10, p. 1245-1254, 2017. (12/50522-0)

SAMMARRO SILVA, KAMILA JESSIE; SABOGAL-PAZ, LYDA PATRICIA. Ferric sulphate flocculation as a concentration method for Giardia and Cryptosporidium in filter backwash water. WATER PRACTICE AND TECHNOLOGY, v. 16, n. 2, p. 557-565, APR 2021. (12/50522-0)

MACIEL, P. M. F.; SABOGAL-PAZ, L. P.. Removal of Giardia spp. and Cryptosporidium spp. from water supply with high turbidity: analytical challenges and perspectives. JOURNAL OF WATER AND HEALTH, v. 14, n. 3, p. 369-378, JUN 2016. (12/50522-0)

MEDEIROS, RAPHAEL CORREA; DANIEL, LUIZ ANTONIO. Comparison of selected methods for recovery of Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater. JOURNAL OF WATER AND HEALTH, v. 13, n. 3, p. 811-818, 2015. (12/50522-0, 10/07902-1, 11/08653-8)

ANDREOLI, FERNANDO CESAR; SABOGAL-PAZ, LYDA PATRICIA. Detection of Giardia and Cryptosporidium in environmental matrices with immunomagnetic separation: two or three acid dissociations. Parasitology Research, v. 120, n. 2, JAN 2021. (12/50522-0)

SAMMARRO SILVA, KAMILA JESSIE; SABOGAL-PAZ, LYDA PATRICIA. Ferric sulphate flocculation as a concentration method for Giardia and Cryptosporidium in filter backwash water. WATER PRACTICE AND TECHNOLOGY, MAR 2021. (12/50522-0)

SAMMARRO SILVA, KAMILA JESSIE; SABOGAL-PAZ, LYDA PATRICIA. Giardia spp. cysts and Cryptosporidium spp. oocysts in drinking water treatment residues: comparison of recovery methods for quantity assessment. ENVIRONMENTAL TECHNOLOGY, v. 42, n. 20, p. 3144-3153, SEP 6 2021. (12/50522-0)

SILVA, HUGO GUILHERME; SABOGAL-PAZ, LYDA PATRICIA. Filter Backwash Water and Floated Residue Containing Pathogenic Protozoa: Detection Method and Treatment Alternatives. WATER AIR AND SOIL POLLUTION, v. 231, n. 3, MAR 13 2020. (12/50522-0)

KONDO NAKADA, LIANE YURI; BUENO FRANCO, REGINA MAURA; DA SILVA FIUZA, VAGNER RICARDO; DOS SANTOS, LUCIANA URBANO; BRANCO, NILSON; GUIMARAES, JOSE ROBERTO. Pre-ozonation of source water: Assessment of efficacy against Giardia duodenalis cysts and effects on natural organic matter. Chemosphere, v. 214, p. 764-770, JAN 2019. (12/50522-0)

BELINI, VALDINEI LUIS; SOUZA FREITAS, BARBARA LUIZA; SABOGAL-PAZ, LYDA PATRICIA; BRANCO, NILSON; BUENO FRANCO, REGINA MAURA. Label-Free Darkfield-Based Technique to Assist in the Detection of Giardia Cysts. WATER AIR AND SOIL POLLUTION, v. 229, n. 6, JUN 2018. (12/50522-0, 14/12712-8)

ANDREOLI, FERNANDO CESAR; SABOGAL-PAZ, LYDA PATRICIA. Coagulation, flocculation, dissolved air flotation and filtration in the removal of Giardia spp. and Cryptosporidium spp. from water supply. ENVIRONMENTAL TECHNOLOGY, v. 40, n. 5, p. 654-663, FEB 23 2019. (12/50522-0)

DOS SANTOS, PRISCILA RIBEIRO; DANIEL, LUIZ ANTONIO. Dissolved air flotation as a potential treatment process to remove Giardia cysts from anaerobically treated sewage. ENVIRONMENTAL TECHNOLOGY, v. 38, n. 19, p. 2392-2399, 2017. (12/50522-0)

GUIMARAES, JOSE ROBERTO; GUADAGNINI, REGIANE APARECIDA; BUENO FRANCO, REGINA MAURA; DOS SANTOS, LUCIANA URBANO. Inactivation of Clostridium perfringens, Total Coliforms, and Escherichia coli by UV/H2O2 in Wastewater Treatment Plant Effluent. JOURNAL OF ADVANCED OXIDATION TECHNOLOGIES, v. 19, n. 1, p. 93-97, JAN 31 2016. (12/50522-0, 10/15550-8)

NASSER FAVA, NATALIA DE MELO; SAMMARRO SILVA, KAMILA JESSIE; SOUZA FREITAS, BARBARA LUIZA; RODRIGUES DA CUNHA, MARIA JULIA; BELINI, VALDINEI LUIS; GERARD DOOLEY, JAMES STEPHEN; FERNANDEZ-IBANEZ, PILAR; BYRNE, JOHN ANTHONY; SABOGAL-PAZ, LYDA PATRICIA. Exploring challenges in Giardia cyst visualisation by common microscopy methods. WATER PRACTICE AND TECHNOLOGY, v. 18, n. 2, p. 9-pg., 2023-01-25. (12/50522-0)

DOS SANTOS, PRISCILA RIBEIRO; LEITE, LUAN DE SOUZA; DANIEL, LUIZ ANTONIO. Performance of biological activated carbon (BAC) filtration for the treatment of secondary effluent: A pilot-scale study. Journal of Environmental Management, v. 302, JAN 15 2022. (12/50522-0)

MEDEIROS, RAPHAEL CORREA; SAMMARRO SILVA, KAMILA JESSIE; DANIEL, LUIZ ANTONIO. Wastewater treatment performance in microbiological removal and (oo)cyst viability assessed comparatively to fluorescence decay. ENVIRONMENTAL TECHNOLOGY, v. 43, n. 7, p. 9-pg., 2020-08-29. (12/50522-0, 11/08653-8, 10/07902-1)

DE OLIVEIRA, GABRIELA LAILA; ERBETTA SUEITT, ANA PAULA; DOS SANTOS, PRISCILA RIBEIRO; LEITE, LUAN DE SOUZA; DANIEL, LUIZ ANTONIO. Removal of protozoan (oo)cysts and bacteria during microalgae harvesting: Outcomes from a lab-scale experiment. Chemosphere, v. 286, n. 3, JAN 2022. (17/14620-1, 15/02073-0, 12/50522-0, 13/50351-4)

OGURA, ALLAN PRETTI; SABOGAL-PAZ, LYDA PATRICIA. Detection and alkaline inactivation of Cryptosporidium spp. oocysts and Giardia spp. cysts in drinking-water treatment sludge. JOURNAL OF WATER PROCESS ENGINEERING, v. 40, APR 2021. (12/50522-0)

OGURA, ALLAN PRETTI; SABOGAL-PAZ, LYDA PATRICIA. Direct centrifugation for detecting Giardia spp. cysts in filter backwash water. JOURNAL OF WATER SANITATION AND HYGIENE FOR DEVELOPMENT, v. 12, n. 6, p. 10-pg., 2022-05-27. (12/50522-0)