Investigation of the cytotoxic, genotoxic, antigenotoxic and antioxidant effects of the Euterpe oleracea Mart. (açaí) oil: analysis in vitro and in vivo; histological, ultra-structural and hematological

• Grant number: 12/17241-8
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2012
• End date: November 30, 2014
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Edson Luis Maistro
• Grantee: Edson Luis Maistro
• Host Institution: Faculdade de Filosofia e Ciências (FFC). Universidade Estadual Paulista (UNESP). Campus de Marília. Marília , SP, Brazil
• Associated researchers: Maria Izabel Souza Camargo

Abstract

Over the years the plants have been increasingly exploited as a source of biologically active compounds. The lack of knowledge about the phitochemical constituents' interactions on biological material can lead to poisonings, disorders on the cellular organization level and on the genetic material in humans. The Euterpe oleracea Mart, popularly known as "açaí", is native from the Amazon region. It is used in folk medicine as an antidiarrheal, for the treatment of anemia, as antihelmintic, astringent and anti-hemorrhagic. Scientific studies on this plant reported anti-inflammatory and antimalarial action, cellular antiproliferative activity and apoptotic, as well as beneficial effects against cardiovascular diseases. Based on the fact that the oil of Euterpe oleracea (OEO) has been traditionally used for medicinal purposes by humans, it is necessary to investigate the toxicological potential of the OEO in relation to the genome and the constitution of mammalian cells. Then, this project was designed to evaluate the cytotoxic, genotoxic, antigenotoxic and antioxidant potential of the oil of Euterpe oleracea in vitro (using human cells - lymphocytes) and in vivo (on different somatic and germ cells of Wistar rats). It will also be investigated the possibility of cellular changes in the liver and thyroid of rats treated with the oil, through histological, ultra-structural, biochemical and hematological analysis in vivo after its administration. This project´s development will allow the implementation of the methodology in our Laboratory of the comet assay nucleoids analysis using TriTek CometScoreTM Freware v1.5 software, as well as to take photograph of the histologic preparations made in collaboration with the UNESP, Rio Claro laboratory, aiming a better characterization of the cytotoxic effects of the compounds. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAISTRO, EDSON LUIS; MARQUES, EDUARDO DE SOUZA; FEDATO, RAFAEL PALHANO; TOLENTINO, FLORA; CEZARIO DA SILVA, CHAYENE DE ANDRADE; FERREIRA TSUBOY, MARCELA STEFANINI; RESENDE, FLAVIA APARECIDA; VARANDA, ELIANA APARECIDA. In Vitro Assessment of Mutagenic And Genotoxic Effects of Coumarin Derivatives 6,7-Dihydroxycoumarin and 4-Methylesculetin. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 78, n. 2, p. 109-118, JAN 17 2015. (12/17241-8, 10/07577-3)

TERRAZAS, PETERSON MENEZES; MARQUES, EDUARDO DE SOUZA; BOLDA MARIANO, LUISA NATHALIA; CECHINEL-FILHO, VALDIR; NIERO, RIVALDO; ANDRADE, SERGIO FALONI; MAISTRO, EDSON LUIS. Benzophenone guttiferone A from Garcinia achachairu Rusby (Clusiaceae) Presents Genotoxic Effects in Different Cells of Mice. PLoS One, v. 8, n. 11, NOV 8 2013. (12/17241-8)

MARQUES, E. S.; FRODER, J. G.; CARVALHO, J. C. T.; ROSA, P. C. P.; PERAZZO, F. F.; MAISTRO, E. L.. Evaluation of the genotoxicity of Euterpe oleraceae Mart. (Arecaceae) fruit oil (acai), in mammalian cells in vivo. Food and Chemical Toxicology, v. 93, p. 13-19, JUL 2016. (12/17241-8)

TOLENTINO, FLORA; DE ARAI, PRISCILA ALVES; MARQUES, EDUARDO DE SOUZA; PETREANU, MARCEL; DE ANDRADE, SERGIO FALONI; NIERO, RIVALDO; PERAZZO, FABIO F.; PIRES ROSA, PAULO CESAR; MAISTRO, EDSON LUIS. In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage. Journal of Ethnopharmacology, v. 164, p. 89-95, APR 22 2015. (12/17241-8)

MARQUES, EDUARDO DE SOUZA; FRODER, JULIANO GABRIEL; DE OLIVEIRA, PATRICIA ROSA; PERAZZO, FABIO FERREIRA; PIRES ROSA, PAULO CESAR; DE MASCARENHAS GAIVAO, ISABEL O'NEILL; CAMARGO MATHIAS, MARIA IZABEL; MAISTRO, EDSON LUIS. Cytotoxic effects of Euterpe oleraceae fruit oil (açaí) in rat liver and thyroid tissues. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 29, n. 1, p. 54-61, JAN-FEB 2019. (12/17241-8)

MARQUES, E. S.; TSUBOY, M. S. F.; CARVALHO, J. C. T.; ROSA, P. C. P.; PERAZZO, F. F.; GAIVAO, I. O. M.; MAISTRO, E. L.. First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (acai) in cultured human cells. Genetics and Molecular Research, v. 16, n. 3, AUG 18 2017. (12/17241-8)