Molecular characterization of Salmonella Typhimurium strains isolated from humans, food and animals in the Southeast and South regions of Brazil

• Grant number: 12/19132-1
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2012
• End date: November 30, 2014
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Juliana Pfrimer Falcão
• Grantee: Juliana Pfrimer Falcão
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The infections caused by Salmonella spp. due to their high occurrence have a great impact on the economy and global public health. Specifically, S. Typhimurium has been reported as the most frequently isolated serovar in the United States, Canada, Australia and New Zealand, and the second most frequently isolated serovar in African, Asian, European and Latin American countries, including Brazil, between 2001 and 2007. Due to the great importance of Salmonella, many phenotypic and genotypic studies have been performed to better elucidate its epidemiology. However, there are just few studies that used molecular methods to type S. Typhimurium strains isolated in Brazil. In this project it will be studied 89 S. Typhimurium, being 49 isolates from humans (43) and food (6), between 1983 and 2010, from various cities of the Sao Paulo State and 40 isolates from animals of the South Region of Brazil. The S. Typhimurium strains will be molecularly type by Pulsed-field gel electrophoresis (PFGE), Multiple-locus variable-number tandem-repeats analysis (MLVA), Multilocus sequence analysis (MLSA) based on the sequences of two virulence genes (sseL e fimH) and two CRISPRs loci (Clustered regularly interspaced short palindromic repeat) and Multilocus sequence typing (MLST) based on housekeeping genes. Moreover, the presence of some virulence markers of the Salmonella Pathogenicity Island (SPI) I and II, and virulence-associated plasmids will be searched by PCR. The set of results to be obtained will contribute for a better characterization of S. Typhimurium regarding its diversity, pathogenic potential and may outline in detail the epidemiology of this serovar of great clinical importance in Brazil and worldwide. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA, FERNANDA; SERIBELLI, AMANDA APARECIDA; DA SILVA, PATRICK; CAZENTINI MEDEIROS, MARTA INES; RODRIGUES, DALIA DOS PRAZERES; MOREIRA, CRISTIANO GALLINA; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Multilocus sequence typing of Salmonella Typhimurium reveals the presence of the highly invasive ST313 in Brazil. INFECTION GENETICS AND EVOLUTION, v. 51, p. 41-44, JUL 2017. (12/19132-1)

FRAZAO, MILIANE R.; ANDRADE, LEONARDO N.; DARINI, ANA L. C.; FALCAO, JULIANA P.. Antimicrobial resistance and plasmid replicons in Yersinia enterocolitica strains isolated in Brazil in 30 years. Brazilian Journal of Infectious Diseases, v. 21, n. 4, p. 477-480, JUL-AUG 2017. (12/19132-1)

ALMEIDA, F.; MEDEIROS, M. I. C.; KICH, J. D.; FALCAO, J. P.. Virulence-associated genes, antimicrobial resistance and molecular typing of Salmonella Typhimurium strains isolated from swine from 2000 to 2012 in Brazil. Journal of Applied Microbiology, v. 120, n. 6, p. 1677-1690, JUN 2016. (12/19132-1)

ALMEIDA, FERNANDA; SERIBELLI, AMANDA APARECIDA; CAZENTINI MEDEIROS, MARTA INES; RODRIGUES, DALIA DOS PRAZERES; DE MELLOVARANI, ALESSANDRO; LUO, YAN; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing. PLoS One, v. 13, n. 8, AUG 13 2018. (12/19132-1, 17/06633-6)

ALMEIDA, FERNANDA; CAZENTINI MEDEIROS, MARTA INES; RODRIGUES, DALIA PRAZERES; PAYNE, JUSTIN; TIMME, RUTH E.; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 40 Salmonella enterica Serovar Typhimurium Strains Isolated from Humans and Food in Brazil. GENOME ANNOUNCEMENTS, v. 4, n. 5, p. 2-pg., 2016-09-01. (12/19132-1)

CAMPIONI, FABIO; CAO, GUOJIE; KASTANIS, GEORGE; LEON, MARIA SANCHEZ; MORATO BERGAMINI, ALZIRA MARIA; RODRIGUES, DALIA DOS PRAZERES; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 256 Salmonella enterica subsp. enterica Serovar Enteritidis Strains Isolated from Humans, Food, Chickens, and Farm Environments in Brazil. GENOME ANNOUNCEMENTS, v. 5, n. 28, p. 6-pg., 2017-07-01. (12/19132-1, 13/25191-3, 16/05817-3)

ALMEIDA, FERNANDA; CAZENTINI MEDEIROS, MARTA INES; RODRIGUES, DALIA DOS PRAZERES; FALCAO, JULIANA PFRIMER. Genotypic diversity, pathogenic potential and the resistance profile of Salmonella Typhimurium strains isolated from humans and food from 1983 to 2013 in Brazil. Journal of Medical Microbiology, v. 64, p. 13-pg., 2015-11-01. (12/19132-1)

ALMEIDA, FERNANDA; CAZENTINI MEDEIROS, MARTA INES; RODRIGUES, DALIA DOS PRAZERES; FALCAO, JULIANA PFRIMER. Genotypic diversity, pathogenic potential and the resistance profile of Salmonella Typhimurium strains isolated from humans and food from 1983 to 2013 in Brazil. Journal of Medical Microbiology, v. 64, n. 11, p. 1395-1407, NOV 2015. (12/19132-1)

ALMEIDA, FERNANDA; CAZENTINI MEDEIROS, MARTA INES; RODRIGUES, DALIA PRAZERES; PAYNE, JUSTIN; TIMME, RUTH E.; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 40 Salmonella enterica Serovar Typhimurium Strains Isolated from Humans and Food in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 5, SEP-OCT 2016. (12/19132-1)

CAMPIONI, FABIO; CAO, GUOJIE; KASTANIS, GEORGE; LEON, MARIA SANCHEZ; MORATO BERGAMINI, ALZIRA MARIA; RODRIGUES, DALIA DOS PRAZERES; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 256 Salmonella enterica subsp. enterica Serovar Enteritidis Strains Isolated from Humans, Food, Chickens, and Farm Environments in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 5, n. 28, JUL 2017. (16/05817-3, 12/19132-1, 13/25191-3)

ALMEIDA, FERNANDA; MEDEIROS, MARTA INES CAZENTINI; RODRIGUES, DALIA DOS PRAZERES; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Molecular characterization of Salmonella Typhimurium isolated in Brazil by CRISPR-MVLST. Journal of Microbiological Methods, v. 133, p. 55-61, FEB 2017. (12/19132-1)

IMORI, PRISCILLA F. M.; PASSAGLIA, JAQUELINE; SOUZA, ROBERTO A.; ROCHA, LENALDO B.; FALCAO, JULIANA P.. Virulence-related genes, adhesion and invasion of some Yersinia enterocolitica-like strains suggests its pathogenic potential. Microbial Pathogenesis, v. 104, p. 72-77, MAR 2017. (10/19830-5, 09/08455-1, 12/19132-1)