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Characterization of Haemophilus parasuis strains from swine through pulsed field electrophoresis (PFGE) and multiloccus sequence typing (MLST)

Grant number: 12/19154-5
Support type:Regular Research Grants
Duration: January 01, 2013 - December 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Andrea Micke Moreno
Grantee:Andrea Micke Moreno
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Haemophilus parasuis is the etiological agent of Glasser's disease; it causes arthritis, pneumonia, meningitis and polyserositis in pigs, and has assumed great importance in modern swine production since their incidence is rising in recent years. Nowadays, fifteen serotypes have been described, however a large number of samples are untypeable by conventional testing. In the present study, a hundred strains of H. parasuis from Laboratory of Swine Health and Virology - VPS - FMVZ -USP culture collection will be submitted to genus/species confirmation and identification of vtaA virulence genes by PCR, strains will be submitted also to serotyping and evaluation of resistance profile by determination of minimal inhibitory concentration. After that, H. parasuis strains will be submitted to molecular characterization by pulsed field electrophoresis and multilocus sequence typing (MLST). Phenotypic and genotypic information of tested strains will be compared to identify of possible epidemiological and virulence markers. (AU)