GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle

• Grant number: 12/50533-2
• Support type: Research Projects - Thematic Grants
• Duration: February 01, 2013 - May 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Cooperation agreement: Innovation Fund Denmark
• Principal researcher: Marcelo Fábio Gouveia Nogueira
• Grantee: Marcelo Fábio Gouveia Nogueira
• Principal researcher abroad: Haja Kadarmideen
• Institution abroad: University of Copenhagen, Denmark
• Home Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
• Associated scholarship(s): 16/16951-2 - Effect of TUDCA on meiosis progression, reactive oxygen species concentration and gene expression on in vitro maturation of bovine cumulus-oocyte complexes, BP.IC; 13/20571-2 - Functional genomics of feed intake and efficiency in Nellore cattle, BP.PD; 13/07730-4 - Use of NPPC, folicular fluid and BMP15 to modulate oocyte meiotic arrest on in vitro maturation: effects on cumulus-oocyte complexes and bovine embryo production, BP.MS; + associated scholarships 13/12097-9 - Genome-wide association studies (GWAS) applied to reproductive traits in Nellore heifers, BP.DR; 13/10473-3 - Role of cell-secreted vesicles in ovarian follicular fluid during oocyte maturation and their involvement in controlling epigenetic changes, BP.PD; 12/23409-9 - Differential gene expression, ultrastructural analysis and evaluation of the lipid profile of in vitro produced bovine blastocysts from oocytes matured conventionally or via SPOM system, BP.DR - associated scholarships

Abstract

Reproductive technologies are important tools in cattle breeding with Artificial Insemination (AI) used for more than 50 years and Embryo Transfer (ET) used since the mid-1980s. More recently, ET of in vitro produced (IVP) embryos derived from ultrasound-guided collection of oocytes (Ovum Pick-Up, OPU) from superior donor cows has been introduced. This technique is used increasingly worldwide, but there are needs for improved understanding of the biology as well as the associated quantitative genetics in order to exploit the full benefits. The aims of the GIFT project are to form a bridge between embryo technology and genetics/genomics in order to address complex biological mechanisms for OPU-IVP traits as well as heifer pregnancy rates, find key genes and genetic markers, and develop genomic selection programs for identification of superior donors and recipients. The GIFT partners (Brazil and Denmark) represent in collaboration a unique set of competences, which synergistically support each other in strong basic genomics and embryological experiences, extensive practical use of the techniques, access to both beef and dairy cattle and two breeds (Bos taurus and Bos indicus), as well as two environments (tropical and temperate climate). Hence, the GIFT project allows for a novel assessment of biological and genetic dimensions of the OPU-IVP technology in a contemporary breeding context. Overall, the GIFT project combines two practical embryo and genomic technologies to make a direct contribution to quality and quantity of meat and milk produced from cattle and used for human consumption. (AU)

Scientific publications

(39)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LIMA, MARINA AMARO; MOROTTI, FABIO; BAYEUX, BERNARDO MARCOZZI; DE REZENDE, ROMULO GERMANO; BOTIGELLI, RAMON CESAR; CAMARA DE BEM, TIAGO HENRIQUE; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; MEIRELLES, FLAVIO VIEIRA; BARUSELLI, PIETRO SAMPAIO; DA SILVEIRA, JULIANO COELHO; PERECIN, FELIPE; SENEDA, MARCELO MARCONDES. Ovarian follicular dynamics, progesterone concentrations, pregnancy rates and transcriptional patterns in Bos indicus females with a high or low antral follicle count. SCIENTIFIC REPORTS, v. 10, n. 1 NOV 11 2020. Web of Science Citations: 0.

PIOLTINE, ELISA M.; MACHADO, MARIANA F.; DA SILVEIRA, JULIANO C.; FONTES, PATRICIA K.; BOTIGELLI, RAMON C.; QUAGLIO, V, ANA ELISA; COSTA, CAMILA B.; NOGUEIRA, MARCELO F. G. Can extracellular vesicles from bovine ovarian follicular fluid modulate the in-vitro oocyte meiosis progression similarly to the CNP-NPR2 system?. Theriogenology, v. 157, p. 210-217, NOV 2020. Web of Science Citations: 0.

CHELES, DORIS SPINOSA; DAL MOLIN, ELOIZA ADRIANE; ROCHA, JOSE CELSO; GOUVEIA NOGUEIRA, MARCELO FABIO. Mining of variables from embryo morphokinetics, blastocyst's morphology and patient parameters: an approach to predict the live birth in the assisted reproduction service. JORNAL BRASILEIRO DE REPRODUCAO ASSISTIDA, v. 24, n. 4, p. 470-479, OCT-DEC 2020. Web of Science Citations: 0.

DE LIMA, CAMILA BRUNA; DOS SANTOS, ERIKA CRISTINA; ISPADA, JESSICA; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; DIAS DOS SANTOS, CHARLES MORPHY; MILAZZOTTO, MARCELLA PECORA. The dynamics between in vitro culture and metabolism: embryonic adaptation to environmental changes. SCIENTIFIC REPORTS, v. 10, n. 1 SEP 24 2020. Web of Science Citations: 0.

LATORRACA, LAIS B.; FEITOSA, WEBER B.; MARIANO, CAMILA; MOURA, MARCELO T.; FONTES, PATRICIA K.; NOGUEIRA, MARCELO F. G.; PAULA-LOPES, FABIOLA F. Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes. SCIENTIFIC REPORTS, v. 10, n. 1 AUG 13 2020. Web of Science Citations: 0.

SAMPAIO, RAFAEL VILAR; SANGALLI, JULIANO RODRIGUES; CAMARA DE BEM, TIAGO HENRIQUE; AMBRIZI, DEWISON RICARDO; DEL COLLADO, MAITE; BRIDI, ALESSANDRA; CAVALCANTE MENDES DE AVILA, ANA CLARA FAQUINELI; MACABELLI, CAROLINA HABERMANN; OLIVEIRA, LILIAN DE JESUS; DA SILVEIRA, JULIANO COELHO; CHIARATTI, MARCOS ROBERTO; PERECIN, FELIPE; BRESSAN, FABIANA FERNANDES; SMITH, LAWRENCE CHARLES; ROSS, PABLO J.; MEIRELLES, FLAVIO VIEIRA. Catalytic inhibition of H3K9me2 writers disturbs epigenetic marks during bovine nuclear reprogramming. SCIENTIFIC REPORTS, v. 10, n. 1 JUL 13 2020. Web of Science Citations: 0.

GRIGOLETTO, LAIS; ALMEIDA SANTANA, MIGUEL HENRIQUE; BRESSAN, FABIANA FERNANDES; ELER, JOANIR PEREIRA; GOUVEIA NOGUEIRA, MARCELO FABIO; KADARMIDEEN, HAJA N.; BARUSELLI, PIETRO SAMPAIO; STERMAN FERRAZ, JOSE BENTO; BRITO, LUIZ F. Genetic Parameters and Genome-Wide Association Studies for Anti-Mullerian Hormone Levels and Antral Follicle Populations Measured After Estrus Synchronization in Nellore Cattle. ANIMALS, v. 10, n. 7 JUL 2020. Web of Science Citations: 0.

FONTES, PATRICIA KUBO; SOUZA CASTILHO, ANTHONY CESAR; RAZZA, EDUARDO MONTANARI; GOUVEIA NOGUEIRA, MARCELO FABIO. Bona fide gene expression analysis of samples from the bovine reproductive system by microfluidic platform. Analytical Biochemistry, v. 596, MAY 1 2020. Web of Science Citations: 0.

BATISTA, EMILIANA O. S.; VIEIRA, LAIS M.; FREITAS, BRUNO G.; GUERREIRO, BRUNA M.; CARVALHO, J. G. S.; MINGOTI, RODOLFO D.; VASCONCELLOS, GUILHERME; SOUZA, ALEXANDRE H.; BENTO STERMAN FERRAZ, JOSE; SAMPAIO BARUSELLI, PIETRO. Anti-Mullerian hormone and its relationship to ovulation response and fertility in timed AI Bos indicus heifers. REPRODUCTION IN DOMESTIC ANIMALS, v. 55, n. 6 APR 2020. Web of Science Citations: 1.

VENTURA, RICARDO V.; BRITO, LUIZ F.; OLIVEIRA, JR., GERSON A.; DAETWYLER, HANS D.; SCHENKEL, FLAVIO S.; SARGOLZAEI, MEHDI; VANDERVOORT, GORDON; FONSECA E SILVA, FABYANO; MILLER, STEPHEN P.; CARVALHO, MINOS E.; SANTANA, MIGUEL H. A.; MATTOS, ELISANGELA C.; FONSECA, PABLO; ELER, JOANIR P.; STERMAN FERRAZ, JOSE BENTO. A comprehensive comparison of high-density SNP panels and an alternative ultra-high-density panel for genomic analyses in Nellore cattle. ANIMAL PRODUCTION SCIENCE, v. 60, n. 3, p. 333-346, 2020. Web of Science Citations: 0.

OLIVEIRA JUNIOR, GERSON A.; SANTOS, DANIEL J. A.; CESAR, ALINE S. M.; BOISON, SOLOMON A.; VENTURA, RICARDO V.; PEREZ, BRUNO C.; GARCIA, JOSE F.; FERRAZ, JOSE BENTO S.; GARRICK, DORIAN J. Fine mapping of genomic regions associated with female fertility in Nellore beef cattle based on sequence variants from segregating sires. JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY, v. 10, n. 1 DEC 16 2019. Web of Science Citations: 0.

GIROTO, ALAN B.; FONTES, PATRICIA K.; FRANCHI, FERNANDA F.; DOS SANTOS, PRISCILA H.; RAZZA, EDUARDO M.; NOGUEIRA, MARCELO F. G.; MAIOLI, MARCOS A.; NOGUEIRA, GUILHERME P.; NUNES, GIOVANA B.; MINGOTI, GISELE Z.; MARECO, EDSON A.; CASTILHO, ANTHONY C. S. Use of pregnancy-associated plasma protein-A during oocyte in vitro maturation increases IGF-1 and affects the transcriptional profile of cumulus cells and embryos from Nelore cows. Molecular Reproduction and Development, v. 86, n. 11, p. 1694-1704, NOV 2019. Web of Science Citations: 0.

VALENTE, RONIELE SANTANA; DE ALMEIDA, TAMIE GUIBU; ALVES, MAYRA FERNANDA; DE CAMARGO, JANINE; BASSO, ANDREA CRISTINA; ANACLETO BELAZ, KATIA ROBERTA; EBERLIN, MARCOS NOGUEIRA; DA CRUZ LANDIM-ALVARENGA, FERNANDA; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; SUDANO, MATEUS JOSE. Modulation of long-chain Acyl-CoA synthetase on the development, lipid deposit and cryosurvival of in vitro produced bovine embryos. PLoS One, v. 14, n. 8 AUG 5 2019. Web of Science Citations: 0.

ANDRADE, GABRIELLA MAMEDE; BOMFIM, MONALISA MEDRADO; DEL COLLADO, MAITE; MEIRELLES, FLAVIO VIEIRA; PERECIN, FELIPE; DA SILVEIRA, JULIANO COELHO. Oxygen tension modulates extracellular vesicles and its miRNA contents in bovine embryo culture medium. Molecular Reproduction and Development, v. 86, n. 8, p. 1067-1080, AUG 2019. Web of Science Citations: 0.

FRANCHI, FERNANDA FAGALI; SATRAPA, RAFAEL AUGUSTO; FONTES, PATRICIA KUBO; SANTOS, PRISCILA HELENA; RAZZA, EDUARDO MONTANARI; EMANUELLI, ISABELE PICADA; ERENO, RONALDO LUIZ; MARECO, EDSON ASSUNCAO; GOUVEIA NOGUEIRA, MARCELO FABIO; BARROS, CIRO MORAES; DE SOUZA CASTILHO, ANTHONY CESAR. Equine chorionic gonadotropin drives the transcriptional profile of immature cumulus-oocyte complexes and in vitro-produced blastocysts of superstimulated Nelore cows. Molecular Reproduction and Development, v. 86, n. 11 AUG 2019. Web of Science Citations: 0.

DALL'ACQUA, PRISCILA CHEDIEK; NUNES, GIOVANA BARROS; DA SILVA, CINTIA RODRIGUES; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; LOPES, FLAVIA LOMBARDI; MARINHO, MARCIA; MINGOTI, GISELE ZOCCAL. Differences in embryonic gene expression and quality indicate the benefit of epidermal growth factor receptor inhibitor during prematuration to improve competence in bovine oocytes. REPRODUCTION IN DOMESTIC ANIMALS, v. 54, n. 4, p. 666-677, APR 2019. Web of Science Citations: 1.

RAZZA, EDUARDO M.; PEDERSEN, HANNE S.; STROEBECH, LOTTE; FONTES, PATRICIA K.; KADARMIDEEN, HAJA N.; CALLESEN, HENRIK; PIHL, MARIA; NOGUEIRA, MARCELO F. G.; HYTTEL, POUL. Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, v. 36, n. 3, p. 413-424, MAR 2019. Web of Science Citations: 1.

ALVES, GLAUCIA PEREIRA; BERTUCCEZ CORDEIRO, FERNANDA; DE LIMA, CAMILA BRUNA; ANNES, KELLY; DOS SANTOS, ERIKA CRISTINA; ISPADA, JESSICA; FONTES, PATRICIA KUBO; GOUVEIA NOGUEIRA, MARCELO FABIO; NICHI, MARCILIO; MILAZZOTTO, MARCELLA PECORA. Follicular environment as a predictive tool for embryo development and kinetics in cattle. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 31, n. 3, p. 451-461, FEB 2019. Web of Science Citations: 0.

DE FIGUEIREDO PESSOA, LAIS VICARI; LISBOA PIRES, PEDRO RATTO; DEL COLLADO, MAITE; GODOY PIERI, NAIRA CAROLINE; RECCHIA, KAIANA; SOUZA, ALINE FERNANDA; PERECIN, FELIPE; DA SILVEIRA, JULIANO COELHO; CESAR DE ANDRADE, ANDRE FURUGEN; AMBROSIO, CARLOS EDUARDO; BRESSAN, FABIANA FERNANDES; MEIRELLES, FLAVIO VIEIRA. Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues. STEM CELLS INTERNATIONAL, 2019. Web of Science Citations: 1.

GOUVEIA NOGUEIRA, MARCELO FIBIO; GUILHERME, VITRIA BERTOGNA; PRONUNCIATE, MICHELI; DOS SANTOS, PRISCILA HELENA; BEZERRA DA SILVA, DIOGO LIMA; ROCHA, JOS CELSO. Artificial Intelligence-Based Grading Quality of Bovine Blastocyst Digital Images: Direct Capture with Juxtaposed Lenses of Smartphone Camera and Stereomicroscope Ocular Lens. SENSORS, v. 18, n. 12 DEC 2018. Web of Science Citations: 0.

SANGALLI, JULIANO RODRIGUES; SAMPAIO, RAFAEL VILAR; DEL COLLADO, MAITE; DA SILVEIRA, JULIANO COELHO; CAMARA DE BEM, TIAGO HENRIQUE; PERECIN, FELIPE; SMITH, LAWRENCE CHARLES; MEIRELLES, FLAVIO VIEIRA. Metabolic gene expression and epigenetic effects of the ketone body beta-hydroxybutyrate. SCIENTIFIC REPORTS, v. 8, SEP 13 2018. Web of Science Citations: 2.

RAZZA, EDUARDO M.; SUDANO, MATEUS J.; FONTES, PATRICIA K.; FRANCHI, FERNANDA F.; BELAZ, KATIA ROBERTA A.; SANTOS, PRISCILA H.; CASTILHO, ANTHONY C. S.; ROCHA, DANIELE F. O.; EBERLIN, MARCOS N.; MACHADO, MARIANA F.; NOGUEIRA, MARCELO F. G. Treatment with cyclic adenosine monophosphate modulators prior to in vitro maturation alters the lipid composition and transcript profile of bovine cumulus-oocyte complexes and blastocysts. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 30, n. 10, p. 1314-1328, SEP 2018. Web of Science Citations: 2.

BOTIGELLI, RAMON CESAR; RAZZA, EDUARDO MONTANARI; PIOLTINE, ELISA MARIANO; FONTES, PATRICIA KUBO; LANCELLOTTI SCHWARZ, KATIA REGINA; VERDE LEAL, CLAUDIA LIMA; GOUVEIA NOGUEIRA, MARCELO FABIO. Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos. REPRODUCTIVE BIOLOGY, v. 18, n. 1, p. 66-75, MAR 2018. Web of Science Citations: 2.

DA SILVEIRA, JULIANO C.; DE AVILA, ANA CLARA F. C. M.; GARRETT, HANNAH L.; BRUEMMER, JASON E.; WINGER, QUINTON A.; BOUMA, GERRIT J. Cell-secreted vesicles containing microRNAs as regulators of gamete maturation. Journal of Endocrinology, v. 236, n. 1, p. R15-R27, JAN 2018. Web of Science Citations: 17.

ROCHA, JOSE CELSO; PASSALIA, FELIPE JOSE; MATOS, FELIPE DELESTRO; TAKAHASHI, MARIA BEATRIZ; MASERATI, JR., MARC PETER; ALVES, MAYRA FERNANDA; DE ALMEIDA, TAMIE GUIBU; CARDOSO, BRUNA LOPES; BASSO, ANDREA CRISTINA; GOUVEIA NOGUEIRA, MARCELO FABIO. Automatized image processing of bovine blastocysts produced in vitro for quantitative variable determination. SCIENTIFIC DATA, v. 4, DEC 19 2017. Web of Science Citations: 4.

ANDRADE, G. M.; MEIRELLES, F. V.; PERECIN, F.; DA SILVEIRA, J. C. Cellular and extracellular vesicular origins of miRNAs within the bovine ovarian follicle. REPRODUCTION IN DOMESTIC ANIMALS, v. 52, n. 6, p. 1036-1045, DEC 2017. Web of Science Citations: 8.

BOMFIM, MONALISA M.; ANDRADE, GABRIELLA M.; DEL COLLADO, MAITE; SANGALLI, JULIANO R.; FONTES, PATRICIA K.; NOGUEIRA, MARCELO F. G.; MEIRELLES, FLAVIO V.; DA SILVEIRA, JULIANO C.; PERECIN, FELIPE. Antioxidant responses and deregulation of epigenetic writers and erasers link oxidative stress and DNA methylation in bovine blastocysts. Molecular Reproduction and Development, v. 84, n. 12, p. 1296-1305, DEC 2017. Web of Science Citations: 6.

DEL COLLADO, MAITE; DA SILVEIRA, JULIANO C.; OLIVEIRA, MARCELO L. F.; ALVES, BARBARA M. S. M.; SIMAS, ROSINEIDE C.; GODOY, ADRIANA T.; COELHO, MIRELA B.; MARQUES, LYGIA A.; CARRIERO, MATEUS M.; NOGUEIRA, MARCELO F. G.; EBERLIN, MARCOS N.; SILVA, LUCIANO A.; MEIRELLES, FLAVIO V.; PERECIN, FELIPE. In vitro maturation impacts cumulus-oocyte complex metabolism and stress in cattle. Reproduction, v. 154, n. 6, p. 881-893, DEC 2017. Web of Science Citations: 3.

OLIVEIRA JUNIOR, G. A.; PEREZ, B. C.; COLE, J. B.; SANTANA, M. H. A.; SILVEIRA, J.; MAZZONI, G.; VENTURA, R. V.; SANTANA JUNIOR, M. L.; KADARMIDEEN, H. N.; GARRICK, D. J.; FERRAZ, J. B. S. Genomic study and Medical Subject Headings enrichment analysis of early pregnancy rate and antral follicle numbers in Nelore heifers. JOURNAL OF ANIMAL SCIENCE, v. 95, n. 11, p. 4796-4812, NOV 2017. Web of Science Citations: 9.

DE BEM, T. H. C.; DA SILVEIRA, J. C.; SAMPAIO, R. V.; SANGALLI, J. R.; OLIVEIRA, M. L. F.; FERREIRA, R. M.; SILVA, L. A.; PERECIN, F.; KING, W. A.; MEIRELLES, F. V.; RAMOS, E. S. Low levels of exosomal-miRNAs in maternal blood are associated with early pregnancy loss in cloned cattle. SCIENTIFIC REPORTS, v. 7, OCT 30 2017. Web of Science Citations: 4.

ANDRADE, GABRIELLA MAMEDE; DA SILVEIRA, JULIANO COELHO; PERRINI, CLAUDIA; DEL COLLADO, MAITE; GEBREMEDHN, SAMUEL; TESFAYE, DAWIT; MEIRELLES, FLAVIO VIEIRA; PERECIN, FELIPE. The role of the PI3K-Akt signaling pathway in the developmental competence of bovine oocytes. PLoS One, v. 12, n. 9 SEP 18 2017. Web of Science Citations: 11.

ROCHA, JOSE CELSO; PASSALIA, FELIPE JOSE; MATOS, FELIPE DELESTRO; TAKAHASHI, MARIA BEATRIZ; CINICIATO, DIEGO DE SOUZA; MASERATI, MARC PETER; ALVES, MAYRA FERNANDA; DE ALMEIDA, TAMIE GUIBU; CARDOSO, BRUNA LOPES; BASSO, ANDREA CRISTINA; GOUVEIA NOGUEIRA, MARCELO FABIO. A Method Based on Artificial Intelligence To Fully Automatize The Evaluation of Bovine Blastocyst Images. SCIENTIFIC REPORTS, v. 7, AUG 9 2017. Web of Science Citations: 9.

MAZZONI, GIANLUCA; PEDERSEN, HANNE S.; DE OLIVEIRA JUNIOR, GERSON A.; ALEXANDRE, PAMELA; RAZZA, EDUARDO M.; CALLESEN, HENRIK; HYTTEL, POUL; NOGUEIRA, MARCELO F. G.; FERRAZ, JOSE BENTO S.; KADARMIDEEN, HAJA N. Application of integrative genomics and systems biology to conventional and in vitro reproductive traits in cattle. ANIMAL REPRODUCTION, v. 14, n. 3, p. 507-513, JUL-SEP 2017. Web of Science Citations: 2.

MAZZONI, GIANLUCA; RAZZA, EDUARDO; PEDERSEN, HANNE S.; SECHER, JAN; KADARMIDEEN, HAJA N.; CALLESEN, HENRIK; STROEBECH, LOTTE; FREUDE, KRISTINE; HYTTEL, POUL. In vitro production of bovine embryos: cumulus/granulosa cell gene expression patterns point to early atresia as beneficial for oocyte competence. ANIMAL REPRODUCTION, v. 14, n. 3, p. 482-489, JUL-SEP 2017. Web of Science Citations: 1.

PEDERSEN, HANNE SKOVSGAARD; MAZZONI, GIANLUCA; STROEBECH, LOTTE; KADARMIDEEN, HAJA N.; HYTTEL, POUL; CALLESEN, HENRIK. Basic and practical aspects of pregnancy establishment in cattle. ANIMAL REPRODUCTION, v. 14, n. 3, p. 581-588, JUL-SEP 2017. Web of Science Citations: 0.

DA SILVEIRA, JULIANO C.; ANDRADE, GABRIELLA M.; DEL COLLADO, MAITE; SAMPAIO, RAFAEL V.; SANGALLI, JULIANO R.; SILVA, LUCIANO A.; PINAFFI, FABIO V. L.; JARDIM, IZABELLE B.; CESAR, MARCELO C.; NOGUEIRA, MARCELO F. G.; CESAR, ALINE S. M.; COUTINHO, LUIZ L.; PEREIRA, RINALDO W.; PERECIN, FELIPE; MEIRELLES, FLAVIO V. Supplementation with small-extracellular vesicles from ovarian follicular fluid during in vitro production modulates bovine embryo development. PLoS One, v. 12, n. 6 JUN 15 2017. Web of Science Citations: 12.

BOTIGELLI, RAMON CESAR; RAZZA, EDUARDO MONTANARI; PIOLTINE, ELISA MARIANO; GOUVEIA NOGUEIRA, MARCELO FABIO. New approaches regarding the in vitro maturation of oocytes: manipulating cyclic nucleotides and their partners in crime. JORNAL BRASILEIRO DE REPRODUCAO ASSISTIDA, v. 21, n. 1, p. 35-44, JAN-MAR 2017. Web of Science Citations: 4.

ROCHA, JOSE C.; PASSALIA, FELIPE; MATOS, FELIPE D.; MASERATI, JR., MARC P.; ALVES, MAYRA F.; DE ALMEIDA, TAMIE G.; CARDOSO, BRUNA L.; BASSO, ANDREA C.; NOGUEIRA, MARCELO F. G. Methods for assessing the quality of mammalian embryos: How far we are from the gold standard?. JORNAL BRASILEIRO DE REPRODUCAO ASSISTIDA, v. 20, n. 3, p. 150-158, JUL-SEP 2016. Web of Science Citations: 6.

DA SILVEIRA, JULIANO C.; DE ANDRADE, GABRIELLA M.; NOGUEIRA, MARCELO F. G.; MEIRELLES, FLAVIO V.; PERECIN, FELIPE. Involvement of miRNAs and Cell-Secreted Vesicles in Mammalian Ovarian Antral Follicle Development. REPRODUCTIVE SCIENCES, v. 22, n. 12, p. 1474-1483, DEC 2015. Web of Science Citations: 12.