Determination of the kinetical parameters for the Aspergillus nidulans glucose transporters hxtBp, hxtCp, and hxtEp

• Grant number: 12/23922-8
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: February 23, 2013
• End date: March 22, 2013
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Gustavo Henrique Goldman
• Grantee: Gustavo Henrique Goldman
• Visiting researcher: Fernando José dos Santos Rodrigues
• Visiting researcher institution: Universidade do Minho (UMinho), Portugal
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 08/56254-2 - Functional analysis of the transcription factor XINR involved in regulation of transcription of cellulases - and hemicellulases-enconding genes in Aspergillus niger, AP.BIOEN.TEM

Abstract

In filamentous fungi, few genes were identified that are related to glucose transport and sensing. In our laboratory, we identified four homologues of S. cerevisiae Snf3 and Rgt2 glucose sensors in A. nidulans named hxtB, hxtC, hxtD e hxtE. The deletion mutants allowed us to characterize them phenotypically. We also evaluated their ability to complement a strain of S. cerevisiae (EBY.VW4000) unable to grow on glucose as single carbon source. The main objective of our project is to understand the transport kinetics associated to hxtBp, hxtCp e hxtEp in the strain EBY.VW4000 that will work as a "cleanroom". Glucose labelling experiments will be determined. (AU)