Comparison between radiological and tomographic methods for evaluation of mechanical damage in seeds

• Grant number: 12/24683-7
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2013
• End date: October 31, 2015
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Francisco Guilhien Gomes Junior
• Grantee: Francisco Guilhien Gomes Junior
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Alberto Tannús ; Carlos Manoel Pedro Vaz ; Fernando Fernandes Paiva ; Lucio Andre de Castro Jorge ; Silvio Moure Cicero

Abstract

Mechanical damages are considered one of the main factors that may adversely affect the seed physiological potential. A significant advancement of knowledge on mechanical damages evaluation in seeds occurred in the late 1990s with the application of X-ray as a nondestructive procedure, allowing to relate the internal injuries occurrence with germination and vigor. However, in some cases the 2D images generated in the radiographic analysis are not sufficient to effectively identify injuries associated with loss of germination, probably for preclude an accurate analysis of the fractures depth. The normal seedlings development even when occurring injuries on the embryonic axis is a major event in the literature that reinforces this limitation of the X-ray test. The application of tomographic methods such as X-ray computed microtomography (microCT) and magnetic resonance (MR), to assess the mechanical damages occurrence in seed using a three-dimensional (3D) scale may help to clarify these subjects. The purpose of this research is to study corn and soybean seeds, with the objectives: (i) optimize the image acquisition parameters through microCT and MR for adequate visualization of the internal seed morphology, (ii) to analyze comparatively the X-ray, microCT and MR images of each seed and to establish a relationship with the results of the germination test. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES-JUNIOR, FRANCISCO GUILHIEN; CICERO, SILVIO MOURE; PEDRO VAZ, CARLOS MANOEL; ORLANDI LASSO, PAULO RENATO. X-ray microtomography in comparison to radiographic analysis of mechanically damaged maize seeds and its effect on seed germination. Acta Scientiarum. Agronomy, v. 41, 2019. (12/24683-7, 15/24008-6)

FONSECA DE OLIVEIRA, GUSTAVO ROBERTO; CICERO, SILVIO MOURE; KRZYZANOWSKI, FRANCISCO CARLOS; GOMES-JUNIOR, FRANCISCO GUILHIEN; BATISTA, THIAGO BARBOSA; FRANCA-NETO, JOSE DE BARROS. Treatment of soybean seeds with mechanical damage: effects on their physiological potential. JOURNAL OF SEED SCIENCE, v. 43, 2021. (12/24683-7, 15/24008-6)