Abstract
Early childhood caries (ECC) is a microbial infection that can severely compromises the dentition of young children. The etiology of ECC includes infection with selected bacteria, and dietary and host factors. In the oral cavity, the components of the innate immune system like antimicrobial cationic peptides (AMCP) are responsible for the maintenance of the ecological balance and the lack of opportunistic infections. Studies have demonstrated that children with ECC have low levels of some AMCP when compared to caries free children. The incorporation of fragments of their peptides in delivery devices could be an interesting way to prevent severe forms of caries in immunologically susceptible children. The objectives of this study will be to evaluate: 1) the in vivo influence of levels of putative cariogenic microorganisms and immunological factors on development of ECC; 2) the in vitro antimicrobial action of synthetic AMCP isolated/combined incorporated or not in nanoparticles systems. In part 1, biofilm will be collected from caries-active and caries-free children for putative cariogenic microorganism counts by Real Time-PCR. The quantification of AMCP using ELISA also will be quantified from saliva samples. In part 2, ATCC and clinical strains will be used to evaluate microbiological functions of synthetic AMCP, MIC/MBC and biofilm assays tests will be developed. The same microbiological tests will be performed for nanoparticles systems containing isolated or combined AMCP, besides toxicity tests on epithelial cells. Data will be statistically analyzed considering p d 0.05. This study intends to contribute to evaluation of the antimicrobial and immunological role of AMCP in the caries prevention and introduce a proposal for incorporation of these peptides in nanoparticle systems as anticaries agents. (AU)
Scientific publications
(6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AIDA, KELLY LIMI;
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FIORAMONTI CALIXTO, GIOVANA MARIA;
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SPOLIDORIO, DENISE M. P.;
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CILLI, EDUARDO MAFFUD;
DUQUE, CRISTIANE.
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Web of Science Citations: 6.
COLOMBO, NATALIA H.;
RIBAS, LAIS F. F.;
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KRESSIRER, CHRISTINE A.;
TANNER, ANNE C. R.;
DUQUE, CRISTIANE.
Antimicrobial peptides in saliva of children with severe early childhood caries.
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COLOMBO, NATALIA HELENA;
PEREIRA, JESSE AUGUSTO;
RODRIGUES DA SILVA, MARJULLY EDUARDO;
FONSECA RIBAS, LAIS FERNANDA;
PARISOTTO, THAIS MANZANO;
MATTOS-GRANER, RENATA DE OLIVEIRA;
SMITH, DANIEL J.;
DUQUE, CRISTIANE.
Relationship between the IgA antibody response against Streptococcus mutans GbpB and severity of dental caries in childhood.
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Web of Science Citations: 5.