Study of the effects of hypertonic saline solution on microcirculatory and cardiac tissue changes, and development of the inflammatory process in a rat model of brain death

• Grant number: 12/19841-2
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2013
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Luiz Felipe Pinho Moreira
• Grantee: Luiz Felipe Pinho Moreira
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Paulina Sannomiya

Abstract

In previous studies it has been shown that brain death itself induces hypoperfusion in the mesenteric microcirculation that is associated with a pronounced reduction in the endogenous corticosterone level, thereby leading to increased local inflammation and organ dysfunction. Furthermore, the autonomic storm does not seem to be responsible for the inflammatory changes associated with brain death, since thoracic epidural anesthesia does not modify the expression of inflammatory mediators, although it effectively blocks the autonomic storm. This study aims to investigate the effects of hypertonic saline solution (7.5%) on microcirculatory changes through the in vivo and in situ observation of the mesenteric microcirculation, and the development of the cardiac and mesenteric inflammatory process in a rat model of brain death. The design includes the investigation of: (1) blood flow, vascular density and leukocyte-endothelial interactions in the mesenteric microcirculation by intra-vital microscopy; (2) expression of P-selectin, ICAM-1, and VCAM-1 on mesenteric microvessels by immunohistochemistry; (3) expression of e-NOS, i-NOS, and endotelin-1 by immunohistochemistry and by real time RT-PCR on the mesentery; (4) expression of ICAM-1 and VCAM-1, caspase-3 and Bcl-2, and a-actin on cardiac tissue by immunohistochemistry; (5) serum and cardiac levels of cytokines by ELISA; (6) serum levels of corticosterone by ELISA; (7) hemodynamic and respiratory parameters, blood gases, electrolytes, and lactate. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORREIA, CRISTIANO DE JESUS; COUTINHO E SILVA, RAPHAEL DOS SANTOS; FERREIRA SOARES, RAFAELA GARCIA; ARMSTRONG JR, ROBERTO; RICARDO-DA-SILVA, FERNANDA YAMAMOTO; SANNOMIYA, PAULINA; BREITHAUPT-FALOPPA, ANA CRISTINA; MOREIRA, LUIZ FELIPE P.. Hypertonic saline reduces cell infiltration into the lungs after brain death in rats. Pulmonary Pharmacology & Therapeutics, v. 61, APR 2020. (12/19841-2)

CORREIA, CRISTIANO DE JESUS; ARMSTRONG, JR., ROBERTO; DE CARVALHO, PRISCILA OLIVEIRA; SIMAS, RAFAEL; JANOLLI SANCHEZ, DANIELA CRISINA; BREITHAUPT-FALOPPA, ANA CRISTINA; SANNOMIYA, PAULINA; PINHO MOREIRA, LUIZ FELIPE. HYPERTONIC SALINE SOLUTION REDUCES MICROCIRCULATORY DYSFUNCTION AND INFLAMMATION IN A RAT MODEL OF BRAIN DEATH. Shock, v. 51, n. 4, p. 495-501, APR 2019. (12/19841-2)

PINTO FERREIRA, LUDMILA RODRIGUES; CORREIA, CRISTIANO JESUS; ZANONI, FERNANDO LUIZ; CARVALHO-SILVA, ANA CAROLINA; ZANIRATTO, RICARDO; CANDIDO, DARLAN DA SILVA; ALMEIDA, RAFAEL RIBEIRO; BREITHAUPT-FALOPPA, ANA CRISTINA; CUNHA-NETO, EDECIO; MOREIRA, LUIZ FELIPE P.. ardiac MicroRNA Expression Profile After Experimental Brain Death Is Associated With Myocardial Dysfunction and Can Be Modulated by Hypertonic Salin. TRANSPLANTATION, v. 106, n. 2, p. 289-298, FEB 2022. (13/50302-3, 12/19841-2, 14/50890-5)

CORREIA, CRISTIANO; MAGGALHAES, DANIEL M.; ZANONI, FERNANDO; SIMAS, RAFAEL; FERREIRA SOARES, RAFAELA GARCIA; COUTINHO E SILVA, RAPHAEL S.; BREITHAUPT-FALOPPA, ANA CRISTINA; SANNOMIYA, PAULINA; MOREIRA, LUIZ FELIPE. EFFECTS OF HYPERTONIC SALINE SOLUTION ON CARDIAC AND PULMONARY CHANGES AFTER BRAIN DEATH: AN EXPERIMENTAL STUDY. Transplant International, v. 30, p. 1-pg., 2017-09-01. (12/19841-2)

MAGALHAES, DANIEL MARCELO S.; ZANONI, FERNANDO LUIZ; CORREIA, CRISTIANO JESUS; SIMAS, RAFAEL; SOARES, RAFAELA GARCIA F.; SANNOMIYA, PAULINA; MOREIRA, LUIZ FELIPE P.. Hypertonic Saline Modulates Heart Function and Myocardial Inflammatory Alterations in Brain-Dead Rats. JOURNAL OF SURGICAL RESEARCH, v. 235, p. 8-15, MAR 2019. (12/19841-2)