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Study of the growth of calcium phosphates and carbonates on organic matrices deposited on mettalic supports: obtainment of osteoinductive biomaterials

Grant number: 12/20946-3
Support Opportunities:Regular Research Grants
Duration: May 01, 2013 - April 30, 2015
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
Principal Investigator:Ana Paula Ramos
Grantee:Ana Paula Ramos
Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated researchers:Pietro Ciancaglini


Biomineralization is the processes from which minerals are formed in living organisms. This process generates complexes systems like bones, tooth, and pearls (nacre), in which organic matrices formed by macromolecules as lipids and proteins are bonded chemicaly and/or physically being responsible for the modifications in the local supersaturation of the ions which origins the nucleation and the growth of the biominerals. In this project, organic matrices formed by phospholipids and proteins by means of the Langmuir-Blodgett (LB) technique, and polyelectrolytes by means of the layer-by-layer (LbL) technique will be deposited on metallic supports, titanium and stainless steel preferentially. These matrices will be used for the growth of calcium carbonates and phosphates from the exposition of the metallic supports containing the matrices to CO2(g) atmosphere and to simulated body fluid solutions, resulting in biomimetic systems. The composition of the organic matrices as well as its surface charge density will be evaluated in interconnection with the type of the biomineral formed on the surfaces. After the complete characterization of the films by means of X-ray difraction, microscopic techniques like scanning electron microscopy and fluorescence microscopy, spectroscopic techniques like vibrational spectroscopy, surface analysis like zeta potential and surface free energy (obtained from contact angle measures), cell viability studies will help in the choice of the best surfaces to be applied to the growth of osteoblastic cells. In the final of the project, it is expected to obtain new hybrid materials that improve and accelerate osteoinduction process, able to be applied in vivo, and potentially patentable. (AU)

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Scientific publications (12)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CRUZ, MARCOS ANTONIO E.; RUIZ, GILIA C. M.; FARIA, AMANDA N.; ZANCANELA, DANIELA C.; PEREIRA, LOURIVALDO S.; CIANCAGLINI, PIETRO; RAMOS, ANA P.. Calcium carbonate hybrid coating promotes the formation of biomimetic hydroxyapatite on titanium surfaces. Applied Surface Science, v. 370, p. 459-468, . (12/22689-8, 12/20946-3)
RUIZ, GILIA C. M.; CRUZ, MARCOS A. E.; FARIA, AMANDA N.; ZANCANELA, DANIELA C.; CIANCAGLINI, PIETRO; RAMOS, ANA P.. Biomimetic collagen/phospholipid coatings improve formation of hydroxyapatite nanoparticles on titanium. Materials Science & Engineering C-Materials for Biological Applications, v. 77, p. 102-110, . (14/11941-3, 12/20946-3)
TOVANI, C. B.; ZANCANELA, D. C.; FARIA, A. N.; CIANCAGLINI, P.; RAMOS, A. P.. Bio-inspired synthesis of hybrid tube-like structures based on CaCO3 and type I-collagen. RSC ADVANCES, v. 6, n. 93, p. 90509-90515, . (13/12615-0, 12/20946-3, 14/24249-0)
NOGUEIRA, LUCAS F. B.; MANIGLIA, BIANCA C.; PEREIRA, LOURIVALDO S.; TAPIA-BLACIDO, DELIA R.; RAMOS, ANA P.. Formation of carrageenan-CaCO3 bioactive membranes. Materials Science & Engineering C-Materials for Biological Applications, v. 58, p. 1-6, . (12/20946-3)
DE SOUZA, ISRAEL D.; CRUZ, MARCOS A. E.; DE FARIA, AMANDA N.; ZANCANELA, DANIELA C.; SIMAO, ANA M. S.; CIANCAGLINI, PIETRO; RAMOS, ANA P.. Formation of carbonated hydroxyapatite films on metallic surfaces using dihexadecyl phosphate-LB film as template. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 118, p. 31-40, . (12/20946-3)
FAVARIN, B. Z.; ANDRADE, M. A. R.; BOLEAN, M.; SIMAO, A. M. S.; RAMOS, A. P.; HOYLAERTS, M. F.; MILLAN, J. L.; CIANCAGLINI, P.. Effect of the presence of cholesterol in the interfacial microenvironment on the modulation of the alkaline phosphatase activity during in vitro mineralization. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 155, p. 466-476, . (16/21236-0, 15/00345-3, 12/20946-3, 14/11941-3, 14/00371-1)
PARRA, GUSTAVO G.; BORISSEVITCH, GALINA; BORISSEVITCH, IOURI; RAMOS, ANA P.. Quantum dot effects upon the interaction between porphyrins and phospholipids in cell membrane models. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, v. 45, n. 3, p. 219-227, . (12/20946-3, 11/20606-5)
ANDRADE, MARCO A. R.; FAVARIN, BRUNO; DERRADI, RAFAEL; BOLEAN, MAYTE; SIMAO, ANA MARIA S.; MILLAN, JOSE LUIS; CIANCAGLINI, PIETRO; RAMOS, ANA P.. Pendant-drop method coupled to ultraviolet-visible spectroscopy: A useful tool to investigate interfacial phenomena. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 504, p. 305-311, . (14/11941-3, 12/20946-3, 15/00345-3)
CRUZ, MARCOS A. E.; DE SOUZA, RAFAEL MAGLIA; DIAS, LUIS GUSTAVO; RAMOS, ANA PAULA. Counterion-mediated Ca2+ accumulation on cationic Langmuir-Blodgett films as template for CaCO3 growth. Thin Solid Films, v. 638, p. 433-440, . (12/22689-8, 12/20946-3, 13/08166-5)
CRUZ, MARCOS ANTONIO E.; RAMOS, ANA P.. Bioactive CaCO3/poly(acrylic acid)/chitosan hybrid coatings deposited on titanium. SURFACE & COATINGS TECHNOLOGY, v. 294, p. 145-152, . (12/22689-8, 12/20946-3)
TOVANI, C. B.; FARIA, A. N.; CIANCAGLINI, P.; RAMOS, A. P.. Collagen-supported CaCO3 cylindrical particles enhance Ti bioactivity. SURFACE & COATINGS TECHNOLOGY, v. 358, p. 858-864, . (13/12615-0, 17/08892-9, 14/24249-0, 16/21236-0, 12/20946-3)
ANDRADE, MARCO A. R.; DERRADI, RAFAEL; SIMAO, ANA M. S.; MILLAN, JOSE LUIS; RAMOS, ANA P.; CIANCAGLINI, PIETRO; BOLEAN, MAYTE. Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?. Archives of Biochemistry and Biophysics, v. 663, p. 192-198, . (17/08892-9, 16/21236-0, 15/00345-3, 12/20946-3, 14/11941-3)

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