Biomineralization is the processes from which minerals are formed in living organisms. This process generates complexes systems like bones, tooth, and pearls (nacre), in which organic matrices formed by macromolecules as lipids and proteins are bonded chemicaly and/or physically being responsible for the modifications in the local supersaturation of the ions which origins the nucleation and the growth of the biominerals. In this project, organic matrices formed by phospholipids and proteins by means of the Langmuir-Blodgett (LB) technique, and polyelectrolytes by means of the layer-by-layer (LbL) technique will be deposited on metallic supports, titanium and stainless steel preferentially. These matrices will be used for the growth of calcium carbonates and phosphates from the exposition of the metallic supports containing the matrices to CO2(g) atmosphere and to simulated body fluid solutions, resulting in biomimetic systems. The composition of the organic matrices as well as its surface charge density will be evaluated in interconnection with the type of the biomineral formed on the surfaces. After the complete characterization of the films by means of X-ray difraction, microscopic techniques like scanning electron microscopy and fluorescence microscopy, spectroscopic techniques like vibrational spectroscopy, surface analysis like zeta potential and surface free energy (obtained from contact angle measures), cell viability studies will help in the choice of the best surfaces to be applied to the growth of osteoblastic cells. In the final of the project, it is expected to obtain new hybrid materials that improve and accelerate osteoinduction process, able to be applied in vivo, and potentially patentable. (AU)
Articles published in Agência FAPESP Newsletter about the research grant:
NOGUEIRA, LUCAS F. B.;
MANIGLIA, BIANCA C.;
PEREIRA, LOURIVALDO S.;
TAPIA-BLACIDO, DELIA R.;
RAMOS, ANA P.
Formation of carrageenan-CaCO3 bioactive membranes.
Materials Science & Engineering C-Materials for Biological Applications,
JAN 1 2016.
Web of Science Citations: 8.