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A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)

Grant number: 13/07121-8
Support type:Regular Research Grants - Publications - Scientific article
Duration: June 01, 2013 - November 30, 2013
Field of knowledge:Biological Sciences - Parasitology
Principal Investigator:Maria Anice Mureb Sallum
Grantee:Maria Anice Mureb Sallum
Home Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The ability to successfully identify and incriminate pathogen vectors is fundamental toeffective pathogen control and management. This task is confounded by the existenceof cryptic species complexes. Molecular markers can offer a highly effective means ofspecies identification in such complexes and are routinely employed in the study ofmedical entomology. Here we evaluate a multi-locus system for the identification ofpotential malaria vectors in the Anopheles strodei subgroup.Larvae, pupae and adult mosquitoes (n=61) from the An. strodei subgroup werecollected from 21 localities in nine Brazilian states and sequenced for the COI, ITS2 andwhite gene. A Bayesian phylogenetic approach was used to describe the relationshipsin the Strodei Subgroup and the utility of COI and ITS2 barcodes was assessed using theneighbor joining tree and "best close match" approaches.Bayesian phylogenetic analysis of the COI, ITS2 and white gene found support forseven clades in the An. strodei subgroup. The COI and ITS2 barcodes were individuallyunsuccessful at resolving and identifying some species in the Subgroup. The COIbarcode failed to resolve An. albertoi and An. strodei but successfully identifiedapproximately 92% of all species queries, while the ITS2 barcode failed to resolve An.arthuri and successfully identified approximately 60% of all species queries. A multilocusCOI-ITS2 barcode, however, resolved all species in a neighbor joining tree andsuccessfully identified all species queries using the "best close match" approach.Our study corroborates the existence of An. albertoi, An. CP Form and An. strodei inthe An. strodei subgroup and identifies four species under An. arthuri informallynamed A-D herein. The use of a multi-locus barcode is proposed for speciesidentification, which has potentially important utility for vector incrimination.Individuals previously found naturally infected with Plasmodium vivax in the southernAmazon basin and reported as An. strodei are likely to have been from An. arthuri Cidentified in this study. (AU)