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Formation and inhibition of compounds generated by oxidative reactions in lipids of Nile Tilapia

Grant number: 13/06489-1
Support type:Regular Research Grants
Duration: June 01, 2013 - July 31, 2015
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Science
Principal Investigator:Neura Bragagnolo
Grantee:Neura Bragagnolo
Home Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


One of the main characteristics of fish lipids is the high content of long chain polyunsaturated fatty acids (PUFA), which are known due to their beneficial health effects. On the other hand, PUFA are highly susceptible to oxidation and can generate trans-4-hydroxy-2-nonenal (HNE) from omega 6 PUFA (PUFA n6) and trans-4-hydroxy-2-hexenal (HHE) from omega 3 PUFA (PUFA n3). The studies that investigate these compounds in food are scarce in the literature, and these compounds need to be studied because they are the most recent markers of lipid oxidation and harmful to health, as well as, to verify which thermal processes will accelerate their formation and means to minimize it. Moreover, fishes, which are food of animal origin, have cholesterol in the composition, which can also undergo oxidation generating cholesterol oxides. The compounds HNE, HHE and cholesterol oxides are considered cytotoxic, mutagenic, carcinogenic and atherogenic, thus, highly prejudicial to public health. One way to delay the lipid oxidation is the addition of antioxidants, for instance, bixin, the main carotenoid of annatto, has proved to have antioxidant capacity. The extraction of lipids is the initial stage for the determination of these compounds and the traditional methods consume a huge amount of solvents which contribute to the environment degradation. Thus, the first aim will be to optimize a method to lipid extraction by microwave assisted extraction (MAE) using less pollutant solvents in small amounts. The second aim will be to verify the formation and inhibition of compounds originated from the oxidation of fish lipids. Samples of Nile tilapia (Sarotherodos niloticus) (cooked at 95ºC for 30 min, grilled at 165ºC for 8 min, baked in electric oven at 180ºC for 10 min and cooked in microwave oven at maximum power for 5 min) will be analyzed. The formation of oxidation compounds due to thermal treatment will be verified by the quantification of content of cholesterol oxides and decomposition compounds of PUFA n6 and n3 (HHE and HNE). In addition, the fatty acid composition, total lipid, moisture and bixin contents will be determined. Initially, the methods to determine total lipids and fatty acids by MAE, cholesterol oxides by GC-MS and the compounds HHE and HNE by HPLC-UV-MS will be optimized. Finally, it will be investigated if there is a correlation between the compounds formed during lipid oxidation. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIRA, GISELDA MACENA; VASCONCELOS QUINTILIANO CABRAL, CATERINE CRISTINE; ARAUJO DE OLIVEIRA, ITALO BRUNO; FIGUEIREDO, BRUNO CHACON; GURGEL BECHTINGER SIMON, SARAH JANAINA; BRAGAGNOLO, NEURA. Changes in the lipid fraction of king mackerel pan fried in coconut oil and cooked in coconut milk. Food Research International, v. 101, p. 198-202, NOV 2017. Web of Science Citations: 2.
SOUZA, HUGO A. L.; MARIUTTI, LILIAN R. B.; BRAGAGNOLO, NEURA. Microwave assisted direct saponification for the simultaneous determination of cholesterol and cholesterol oxides in shrimp. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, v. 169, n. SI, p. 88-95, MAY 2017. Web of Science Citations: 4.
VENANCIO COSTA, DEBORA DOS SANTOS; BRAGAGNOLO, NEURA. Development and validation of a novel microwave assisted extraction method for fish lipids. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, v. 119, n. 3 MAR 2017. Web of Science Citations: 6.
BARRIUSO, BLANCA; MARIUTTI, LILIAN REGINA BARROS; ANSORENA, DIANA; ASTIASARAN, ICIAR; BRAGAGNOLO, NEURA. Solanum sessiliflorum (mana-cubiu) antioxidant protective effect toward cholesterol oxidation: Influence of docosahexaenoic acid. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, v. 118, n. 8, p. 1125-1131, AUG 2016. Web of Science Citations: 3.
FIGUEIREDO, BRUNO C.; BRAGAGNOLO, NEURA; SKIBSTED, LEIF H.; ORLIEN, VIBEKE. Inhibition of Cholesterol and Polyunsaturated Fatty Acids Oxidation through the Use of Annatto and Bixin in High-Pressure Processed Fish. Journal of Food Science, v. 80, n. 8, p. C1646-C1653, AUG 2015. Web of Science Citations: 8.
FIGUEIREDO, BRUNO CHACON; TRAD, ISABELA JORGE; BARROS MARIUTTI, LILIAN REGINA; BRAGAGNOLO, NEURA. Effect of annatto powder and sodium erythorbate on lipid oxidation in pork loin during frozen storage. Food Research International, v. 65, n. B, SI, p. 137-143, NOV 2014. Web of Science Citations: 8.

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