Effect of energy density of the diet and chromium supplementation on serum concentrations of glucose, insulin, non-esterified fatty acids, triglycerides and IGF-1 on lactating dairy cows

• Grant number: 12/24816-7
• Support Opportunities: Regular Research Grants
• Duration: June 01, 2013 - June 30, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: José Luiz Moraes Vasconcelos
• Grantee: José Luiz Moraes Vasconcelos
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Reinaldo Fernandes Cooke

Abstract

The objective of this study is to evaluate the influence of the energy density of the diet and chromium supplementation on the serum concentrations of glucose, insulin, non-esterified fatty acids (NEFA), triglycerides (TG) and insulin like growth factor 1 (IGF-1) lactating cows. Eighteen of crossbred Holstein x Gir cows, allocated in two pastures and receiving free-choice of corn silage and water, will be blocked by parturition (primiparous x multiparous) and milk yield, and randomly assigned to one of three treatments on d0 of the experiment: 1) Control: diet formulated to reach 100% of requirements for maintenance and lactation, without chromium supplementation (n=6); 2) GPSC: diet formulated to reach 100% and 160% of the requirements for lactation and maintenance, respectively, without chromium supplementation (n=6); 3) GPCC: diet formulated to meet 100% and 160% of the requirements for lactation and maintenance requirements, respectively, with chromium supplementation (n=6; 2,5g of KemTRACETM Chromium Propionate 0,4% - Kemin Industries, Inc. - added in 97,5g of corn meal/animal/day). Experimental diets will be fed twice daily. Treatments will be analyzed as contrast to indentify major contrast responses: Control versus GPSC to determine the effect of the energy density, and GPSC versus GPCC to determine the effect of chromium supplementation in high energy diets. Glucose Tolerance Test will be performed to determine the glucose clearance rate and insulin release. Oocytes will picked-up to determine their quality. All variables will be analyzed using the MIXED procedure of SAS (Inst., Inc., Cary, NC) and cow as experimental unit. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COOKE, R. F.; CAPPELLOZZA, B. I.; GUARNIERI FILHO, T. A.; DEPNER, C. M.; LYTLE, K. A.; JUMP, D. B.; BOHNERT, D. W.; CERRI, R. L. A.; VASCONCELOS, J. L. M.. Effects of calcium salts of soybean oil on factors that influence pregnancy establishment in Bos indicus beef cows. JOURNAL OF ANIMAL SCIENCE, v. 92, n. 5, p. 2239-2250, MAY 2014. (12/24816-7, 12/16856-9)

LEIVA, T.; COOKE, R. F.; BRANDAO, A. P.; ABOIN, A. C.; RANCHES, J.; VASCONCELOS, J. L. M.. Effects of excessive energy intake and supplementation with chromium propionate on insulin resistance parameters, milk production, and reproductive outcomes of lactating dairy cows. LIVESTOCK SCIENCE, v. 180, p. 121-128, OCT 2015. (12/24816-7, 12/25390-3)