Abstract
Leukotrienes (LTs) and prostaglandines (PGs) regulate the immune response in infections and other pathologies. In last years, we have contributed for the understanding of the role of LTs and PGs in infections and vaccinal processes. We have shown that LTs are essential for defense mechanisms in histoplasmosis, tuberculosis, and strongyloidiasis, acting as adjuvants and recruiting effector cells. However, these mediators favor infections in trypanosomiasis and candidiasis. Referring to PGs, we have shown that these contribute for pathogenesis of histoplasmosis and strongyloidiasis. Knowing that LTs and PGs are unstable, we have developed a methodology of encapsulation in biocompatible polymers, and we have shown that this process preserves their biological activities, thus representing a new strategy for therapeutic intervention. In this project, formed by 09 subprojects, we will proceed the studies on LTs and PGs in the regulation of innate or induced immune response and the use of these mediators in therapies. In this context, our objectives are (a) to investigate the role of LTs and PGs in the effector mechanisms of macrophages infected by Cronobacter sakazakii; (b) to determine the relationship among lipidic mediators and (b1) infectivity/pathogenicity of strains of Mycobacterium tuberculosis isolated from patients with different clinical forms of the disease, (b2) susceptibility and resistance in the infection by Histoplasma capsulatum, (b3) different methods of vaccination ("prime-booster", gene transfection by electroporation or bioballistics); (c) to develop formulations containing lipidic mediators or their inhibitors associated or not to antigens as vaccinal or therapeutic strategies. Our studies shall widen the understanding on effector mechanisms of immune response that are regulated by LTs and PGs, contribute for development of new therapeutic strategies, form human resources in multidisciplinary areas, and set new methodologies in the College of Pharmaceutical Science of Ribeirão Preto. (AU)
Scientific publications
(15)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SORGI, CARLOS ARTERIO;
SOARES, ELYARA MARIA;
ROSADA, ROGERIO SILVA;
BITENCOURT, CLAUDIA SILVA;
ZOCCAL, KARINA FURLANI;
TARTARI PEREIRA, PRISCILLA APARECIDA;
FONTANARI, CAROLINE;
BRANDAO, IZAIRA;
MASSON, ANA PAULA;
RAMOS, SIMONE GUSMAO;
SILVA, CELLO LOPES;
FRANTZ, FABIANI GAI;
FACCIOLI, LUCIA HELENA.
Eicosanoid pathway on host resistance and inflammation during Mycobacterium tuberculosis infection is comprised by LTB4 reduction but not PGE(2) increment.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,
v. 1866,
n. 3
MAR 1 2020.
Web of Science Citations: 0.
PEREIRA, PRISCILLA A. T.;
BITENCOURT, CLAUDIA S.;
REIS, MOUZARLLEM B.;
FRANTZ, FABIANI G.;
SORGI, CARLOS A.;
SOUZA, CAMILA O. S.;
SILVA, CELIO L.;
GARDINASSI, LUIZ G.;
FACCIOLI, LUCIA H.
Immunomodulatory activity of hyaluronidase is associated with metabolic adaptations during acute inflammation.
Inflammation Research,
v. 69,
n. 1
NOV 2019.
Web of Science Citations: 0.
PEREIRA, PRISCILLA A. T.;
ASSIS, PATRICIA A.;
PRADO, MORGANA K. B.;
RAMOS, SIMONE G.;
ARONOFF, DAVID M.;
DE PAULA-SILVA, FRANCISCO W. G.;
SORGI, CARLOS A.;
FACCIOLI, LUCIA H.
Prostaglandins D-2 and E-2 have opposite effects on alveolar macrophages infected with Histoplasma capsulatum.
Journal of Lipid Research,
v. 59,
n. 2,
p. 195-206,
FEB 2018.
Web of Science Citations: 5.
FRONZA, MARCIO;
MUHR, CORNELIA;
CALHEIROS DA SILVEIRA, DENISE SAYURI;
SORGI, CARLOS ARTERIO;
DE PAULA RODRIGUES, STEPHEN FERNANDES;
POLISELLI FARSKY, SANDRA HELENA;
GARCIA PAULA-SILVA, FRANCISCO WANDERLEY;
MERFORT, IRMGARD;
FACCIOLI, LUCIA HELENA.
Hyaluronidase decreases neutrophils infiltration to the inflammatory site.
Inflammation Research,
v. 65,
n. 7,
p. 533-542,
JUL 2016.
Web of Science Citations: 6.
PUCCA, MANUELA B.;
PEIGNEUR, STEVE;
COLOGNA, CAMILA T.;
CERNI, FELIPE A.;
ZOCCAL, KARINA F.;
BORDON, KARLA DE C. F.;
FACCIOLI, LUCIA H.;
TYTGAT, JAN;
ARANTES, ELIANE C.
Electrophysiological characterization of the first Tityus serrulatus alpha-like toxin, Ts5: Evidence of a pro-inflammatory toxin on macrophages.
Biochimie,
v. 115,
p. 8-16,
AUG 2015.
Web of Science Citations: 12.
ZOCCAL, KARINA FURLANI;
GARCIA PAULA-SILVA, FRANCISCO WANDERLEY;
BITENCOURT, CLAUDIA DA SILVA;
SORGI, CARLOS ARTERIO;
FIGUEIREDO BORDON, KARLA DE CASTRO;
ARANTES, ELIANE CANDIANI;
FACCIOLI, LUCIA HELENA.
PPAR-gamma activation by Tityus serrulatus venom regulates lipid body formation and lipid mediator production.
Toxicon,
v. 93,
p. 90-97,
JAN 2015.
Web of Science Citations: 15.
LOCACHEVIC, GISELE APARECIDA;
TARTARI PEREIRA, PRISCILLA APARECIDA;
SECATTO, ADRIANA;
FONTANARI, CAROLINE;
GALVAO, ALYNE FAVERO;
BORGES PRADO, MORGANA KELLY;
ZOCCAL, KARINA FURLANI;
PETTA, TANIA;
BERALDO MORAES, LUIZ ALBERTO;
RAMOS, SIMONE GUSMAO;
DE CASTRO, FABOLA ATTIE;
SORGI, CARLOS ARTERIO;
FACCIOLI, LUCIA HELENA.
Erythropoietin Exacerbates Inflammation and Increases the Mortality of Histoplasma capsulatum-Infected Mice.
Mediators of Inflammation,
2015.
Web of Science Citations: 3.
FRONZA, MARCIO;
CAETANO, GUILHERME F.;
LEITE, MARCEL N.;
BITENCOURT, CLAUDIA S.;
PAULA-SILVA, FRANCISCO W. G.;
ANDRADE, THIAGO A. M.;
FRADE, MARCO A. C.;
MERFORT, IRMGARD;
FACCIOLI, LUCIA H.
Hyaluronidase Modulates Inflammatory Response and Accelerates the Cutaneous Wound Healing.
PLoS One,
v. 9,
n. 11
NOV 13 2014.
Web of Science Citations: 23.
ASSIS, PATRICIA A.;
ESPINDOLA, MILENA S.;
PAULA-SILVA, FRANCISCO W. G.;
RIOS, WENDY M.;
PEREIRA, PRISCILLA A. T.;
LEAO, SYLVIA C.;
SILVA, CELIO L.;
FACCIOLI, LUCIA H.
Mycobacterium tuberculosis expressing phospholipase C subverts PGE(2) synthesis and induces necrosis in alveolar macrophages.
BMC Microbiology,
v. 14,
MAY 19 2014.
Web of Science Citations: 14.
ESPINDOLA, MILENA SOBRAL;
FRANTZ, FABIANI GAI;
SOARES, LUANA SILVA;
MASSON, ANA PAULA;
TEFE-SILVA, CRISTIANE;
BITENCOURT, CLAUDIA SILVA;
OLIVEIRA, SERGIO COSTA;
RODRIGUES, VANDERLEI;
RAMOS, SIMONE GUSMAO;
SILVA, CELIO LOPES;
FACCIOLI, LUCIA HELENA.
Combined immunization using DNA-Sm14 and DNA-Hsp65 increases CD8+memory T cells, reduces chronic pathology and decreases egg viability during Schistosoma mansoni infection.
BMC INFECTIOUS DISEASES,
v. 14,
MAY 16 2014.
Web of Science Citations: 5.
ZOCCAL, KARINA FURLANI;
BITENCOURT, CLAUDIA DA SILVA;
GARCIA PAULA-SILVA, FRANCISCO WANDERLEY;
SORGI, CARLOS ARTERIO;
FIGUEIREDO BORDON, KARLA DE CASTRO;
ARANTES, ELIANE CANDIANI;
FACCIOLI, LUCIA HELENA.
TLR2, TLR4 and CD14 Recognize Venom-Associated Molecular Patterns from Tityus serrulatus to Induce Macrophage-Derived Inflammatory Mediators.
PLoS One,
v. 9,
n. 2
FEB 7 2014.
Web of Science Citations: 31.
ZOCCAL, KARINA FURLANI;
BITENCOURT, CLAUDIA DA SILVA;
SECATTO, ADRIANA;
SORGI, CARLOS ARTERIO;
FIGUEREDO BORDON, KARLA DE CASTRO;
SAMPAIO, SUELY VILELA;
ARANTES, ELIANE CANDIANI;
FACCIOLI, LUCIA HELENA.
Tityus serrulatus venom and toxins Ts1, Ts2 and Ts6 induce macrophage activation and production of immune mediators.
Toxicon,
v. 57,
n. 7-8,
p. 1101-1108,
JUN 2011.
Web of Science Citations: 42.